BBa_K1590006
1
BBa_K1590006
Human Lanosterol Synthase
2015-09-09T11:00:00Z
2015-09-18T04:06:15Z
In silico optimised version for E. coli K12 strains. Sequence ordered through IDT.
Lanosterol Synthase catalyses the reaction from 2,3-oxido-squalene (squalene epoxide) to Lanosterol. It is one of the enzymes in the enzymatic cascade that converts squalene to cholesterol through stepwise modifications of the substrate.
false
false
_2007_
8083
24715
9
false
Choosing the correct codon-bias, remove 'illegal' restriction sites, ensure that (stop)codons are in frame, addition of standard prefix and suffix.
Intermediate primers for sequencing Lanosterol Synthase.
Forward: GGCTGTTCCCGGACTGGG
Reverse: CGGTGACATCATGATCGAC
Primers for cloning Lanosterol Synthase into overexpression vector pQE80-L.
Forward: GCGCGGATCCATGACCGAAGGAACCTGC
Reverse: GCGCGGTACCTTACGGGTGACCCGCCAGCGC
Part was synthesised with appropriate prefix/suffix sequences, hence no separate primers were required for cloning it into pSB1C3.
false
Manuel Blank
annotation2448551
1
Human Lanosterol Synthase Protein Coding Sequence
range2448551
1
1
2196
annotation2448550
1
ATG start codon
range2448550
1
1
3
annotation2448552
1
TAA stop codon
range2448552
1
2197
2199
BBa_K1590006_sequence
1
atgaccgaaggaacctgcctgcgtcgtcgtggtggtccgtacaaaaccgaaccggcgaccgacctgggtcgttggcgtctgaactgcgaacgtggtcgtcagacctggacctacctacaggacgaacgtgcgggtcgtgaacagaccggtctggaagcgtacgcgctgggtctggacaccaaaaactacttcaaagacctgccgaaagcgcacaccgcgttcgaaggtgcgctgaacggtatgaccttctacgttggtctacaggcggaagacggtcactggaccggtgactacggtggtccgctgttcctgctgccgggtctgctgatcacctgccacgttgcgcgtatcccgctgccagctggttaccgagaggaaattgtccgatatctacgatcagtacaactgccggacggtggttggggtctgcacatcgaagacaaatctaccgttttcggaaccgcgctgaactacgtttctctgcgtatcctgggtgttggtccggacgacccggacctggttcgtgcgcgtaacatcctgcacaaaaaaggtggtgcggttgcgatcccgtcttggggtaaattctggctggcggttctgaacgtttactcttgggaaggtctgaacaccctgttcccggaaatgtggctgttcccggactgggcgccggcgcacccgtctaccctgtggtgccactgccgtcaggtttacctgccgatgtcttactgctacgcggttcgtctgtctgcggcggaagacccgctggttcagtctctgcgtcaggaactgtacgttgaagacttcgcgtctatagattggctagcacaacgtaacaatgttgctccagatgaactgtacaccccgcactcttggctgctgcgtgttgtttacgcgctgctgaacctgtacgaacaccaccactctgcgcacctgcgtcagcgtgcggttcagaaactgtacgaacacatcgttgcggacgaccgtttcaccaaatctatctctatcggtccgatctctaaaaccatcaacatgctggttcgttggtacgttgacggtccggcgtctaccgcgttccaggaacacgtttctcgtatcccggactacctgtggatgggtctggacggtatgaaaatgcagggcaccaacggttctcagatatgggacaccgcgttcgcgatccaggcgctgctggaagcgggtggtcaccaccgtccggagttctcttcttgcctacagaaagcgcacgagttcctgcgtctgtctcaggttccagataatccacctgattaccagaaatactaccgtcagatgcgtaaaggtggtttctctttctctaccctggactgcggttggatcgtttctgactgcaccgcggaagcgctgaaagcggttctgctgctacaggaaaaatgcccgcacgttaccgaacacatcccgcgtgaacgtctgtgcgacgcggttgcggttctgctgaacatgcgtaacccggacggtggtttcgcgacctacgaaaccaaacgtggtggtcacctgctggaactgctgaacccgtctgaagttttcggtgacatcatgatcgactacacctatgttgaatgcaccagtgcggtgatgcaggcattaaaatacttccacaagcgctttccggaacaccgcgcagcggaaatccgcgaaacgttgacccaaggtctggagttctgccgtcgtcagcagcgtgcggacggttcttgggaaggttcttggggtgtttgcttcacctacggcacctggttcggtctggaagcgttcgcgtgcatgggtcagacctaccgtgacggaaccgcgtgcgcggaagtttctcgtgcgtgcgacttcctgctgtctcgtcagatggcggacggtggttggggtgaagacttcgaatcttgcgaagaacgtcgttacctacagtctgcgcagtctcagatccacaacacctgctgggcgatgatgggtctgatggcggttcgtcacccggacatcgaagcgcaggaacgtggtgttcgttgcctgctggaaaaacagctgccgaacggtgactggccgcaggaaaacatcgcgggtgttttcaacaaatcttgcgcgatctcttacacctcttaccgtaacatcttcccgatctgggcgctgggtcgtttctctcagctgtacccggaacgtgcgctggcgggtcacccgtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z