BBa_K1590006 1 BBa_K1590006 Human Lanosterol Synthase 2015-09-09T11:00:00Z 2015-09-18T04:06:15Z In silico optimised version for E. coli K12 strains. Sequence ordered through IDT. Lanosterol Synthase catalyses the reaction from 2,3-oxido-squalene (squalene epoxide) to Lanosterol. It is one of the enzymes in the enzymatic cascade that converts squalene to cholesterol through stepwise modifications of the substrate. false false _2007_ 8083 24715 9 false Choosing the correct codon-bias, remove 'illegal' restriction sites, ensure that (stop)codons are in frame, addition of standard prefix and suffix. Intermediate primers for sequencing Lanosterol Synthase. Forward: GGCTGTTCCCGGACTGGG Reverse: CGGTGACATCATGATCGAC Primers for cloning Lanosterol Synthase into overexpression vector pQE80-L. Forward: GCGCGGATCCATGACCGAAGGAACCTGC Reverse: GCGCGGTACCTTACGGGTGACCCGCCAGCGC Part was synthesised with appropriate prefix/suffix sequences, hence no separate primers were required for cloning it into pSB1C3. false Manuel Blank annotation2448552 1 TAA stop codon range2448552 1 2197 2199 annotation2448551 1 Human Lanosterol Synthase Protein Coding Sequence range2448551 1 1 2196 annotation2448550 1 ATG start codon range2448550 1 1 3 BBa_K1590006_sequence 1 atgaccgaaggaacctgcctgcgtcgtcgtggtggtccgtacaaaaccgaaccggcgaccgacctgggtcgttggcgtctgaactgcgaacgtggtcgtcagacctggacctacctacaggacgaacgtgcgggtcgtgaacagaccggtctggaagcgtacgcgctgggtctggacaccaaaaactacttcaaagacctgccgaaagcgcacaccgcgttcgaaggtgcgctgaacggtatgaccttctacgttggtctacaggcggaagacggtcactggaccggtgactacggtggtccgctgttcctgctgccgggtctgctgatcacctgccacgttgcgcgtatcccgctgccagctggttaccgagaggaaattgtccgatatctacgatcagtacaactgccggacggtggttggggtctgcacatcgaagacaaatctaccgttttcggaaccgcgctgaactacgtttctctgcgtatcctgggtgttggtccggacgacccggacctggttcgtgcgcgtaacatcctgcacaaaaaaggtggtgcggttgcgatcccgtcttggggtaaattctggctggcggttctgaacgtttactcttgggaaggtctgaacaccctgttcccggaaatgtggctgttcccggactgggcgccggcgcacccgtctaccctgtggtgccactgccgtcaggtttacctgccgatgtcttactgctacgcggttcgtctgtctgcggcggaagacccgctggttcagtctctgcgtcaggaactgtacgttgaagacttcgcgtctatagattggctagcacaacgtaacaatgttgctccagatgaactgtacaccccgcactcttggctgctgcgtgttgtttacgcgctgctgaacctgtacgaacaccaccactctgcgcacctgcgtcagcgtgcggttcagaaactgtacgaacacatcgttgcggacgaccgtttcaccaaatctatctctatcggtccgatctctaaaaccatcaacatgctggttcgttggtacgttgacggtccggcgtctaccgcgttccaggaacacgtttctcgtatcccggactacctgtggatgggtctggacggtatgaaaatgcagggcaccaacggttctcagatatgggacaccgcgttcgcgatccaggcgctgctggaagcgggtggtcaccaccgtccggagttctcttcttgcctacagaaagcgcacgagttcctgcgtctgtctcaggttccagataatccacctgattaccagaaatactaccgtcagatgcgtaaaggtggtttctctttctctaccctggactgcggttggatcgtttctgactgcaccgcggaagcgctgaaagcggttctgctgctacaggaaaaatgcccgcacgttaccgaacacatcccgcgtgaacgtctgtgcgacgcggttgcggttctgctgaacatgcgtaacccggacggtggtttcgcgacctacgaaaccaaacgtggtggtcacctgctggaactgctgaacccgtctgaagttttcggtgacatcatgatcgactacacctatgttgaatgcaccagtgcggtgatgcaggcattaaaatacttccacaagcgctttccggaacaccgcgcagcggaaatccgcgaaacgttgacccaaggtctggagttctgccgtcgtcagcagcgtgcggacggttcttgggaaggttcttggggtgtttgcttcacctacggcacctggttcggtctggaagcgttcgcgtgcatgggtcagacctaccgtgacggaaccgcgtgcgcggaagtttctcgtgcgtgcgacttcctgctgtctcgtcagatggcggacggtggttggggtgaagacttcgaatcttgcgaagaacgtcgttacctacagtctgcgcagtctcagatccacaacacctgctgggcgatgatgggtctgatggcggttcgtcacccggacatcgaagcgcaggaacgtggtgttcgttgcctgctggaaaaacagctgccgaacggtgactggccgcaggaaaacatcgcgggtgttttcaacaaatcttgcgcgatctcttacacctcttaccgtaacatcttcccgatctgggcgctgggtcgtttctctcagctgtacccggaacgtgcgctggcgggtcacccgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z