BBa_K1590007 1 BBa_K1590007 Human Odorant Binding Protein 2A 2015-09-07T11:00:00Z 2015-09-18T04:07:54Z In silico optimised version for E. coli K12 strains. Sequence ordered through IDT. Human Odorant Binding Protein 2A is ... false false _2007_ 8083 24715 9 false Choosing the correct codon-bias, removing 'illegal' restriction sites, ensure that codons are in frame, addition of standard prefix and suffix. Primers for cloning Odorant Binding Protein 2A into overexpression vector pQE80-L. Forward: GCGC GAATTCGCGGCCGCTTCTAG A TGCTGTCTTTCACCCTGGAAGAA Reverse: GCGC CTGCAGCGGCCGCTACTAGT ATTATTAGTGTTCCAGAACGCAAG <!--Part was synthesised with appropriate prefix/suffix sequences, hence no separate primers were required for cloning it into pSB1C3? --> false Conner Craigon annotation2447435 1 ATG start codon range2447435 1 1 3 annotation2447436 1 Human Odorant Binding Protein 2A Protein Coding Sequence range2447436 1 1 468 BBa_K1590007_sequence 1 atgctgtctttcaccctggaagaagaagacatcaccggcacctggtacgttaaagcgatggttgttgacaaagacttcccggaagaccgtcgtccgcgtaaagtttctccggttaaagttaccgcgctgggtggtggtaacctggaagcgaccttcaccttcatgcgtgaagaccgttgcatccagaaaaaaatcctgatgcgtaaaaccgaagaaccgggtaaattctctgcgtacggtggtcgtaaactgatctacctgcaagaactgccgggcaccgacgactacgttttctactgcaaagaccagcgtcgtggtggtctgcgttacatgggtaaactggttggtcgtaacccgaacaccaacctggaagcgctggaagaatttaaaaaactggttcagcacaaaggtctgtctgaagaagacatcttcatgccgctgcaaaccggttcttgcgttctggaacac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z