BBa_K1590008 1 BBa_K1590008 Lactoferrin Binding Protein 2015-09-08T11:00:00Z 2015-09-18T04:08:31Z PCR amplification of Neisseria Meningitidis MC58 gDNA. gDNA provided by James Moir - University of York. Coding sequence for Lactoferrin Binding Protein of Neisseria Meningitidis. This protein sequesters Iron for the host from Lactoferrin. false false _2007_ 8083 24715 9 false Biobrick Prefix, Suffix, and TAA stop codons had to be added. Sequence was checked for forbidden restriction sites in order to comply with assembly standard RFC10. Forward primer for amplifying lactoferrin binding protein A from Neisseria meningitidis MC58 gDNA. Forward: GCGC GAATTCGCGGCCGCTTCTAG A TGAATAAGAAACACGGTTTTCCG Reverse: GCGC CTGCAGCGGCCGCTACTAGT ATTATT AAAACTTCATTTCGAGCG Intermediate sequencing primers for Lactoferrin Binding Protein. Forward 1: GAAGTCGCTCGGCGCGGGG Forward 2: GACAAGAGCGGGGTGTACG Reverse : GCCGAATCAGGCAGGAC Primers for cloning Lactoferrin Binding Protein into overexpression vector pQE80-L. Forward: GCGC GGATCC AATAAGAAACACGGTTTTCC Reverse: GCGC GGTACC TTAAAACTTCATTTCGAGCG false Manuel Blank annotation2447562 1 TAA stop codon range2447562 1 2830 2832 annotation2447561 1 Lactoferrin Binding Protein A Coding Sequence range2447561 1 1 2829 annotation2447560 1 ATG start codon range2447560 1 1 3 BBa_K1590008_sequence 1 atgaataagaaacacggttttccgctgactctgactgccttggccattgcaaccgcttttccggcttatgctgcccaagcggggggggcgacacctgatgccgcgcagacccaatccctgaaagagattaccgtccgtgccgccaaagtgggacggcgatcgaaagaggcgaccggtttgggcaaaatcgtcaaaacgtcggaaacgttgaacaaagaacaggtactcggtatccgcgacctgacgcgctacgatccgggcgtggcggttgtcgaacagggcaacggcgcgagcggcggctactcgatacgcggcgtggataaaaaccgtgtggcggtttcggtcgacggtgttgcccaaatacaggcgtttaccgtgcagggatcgttgagcggatacggcggacgcggcggcagcggtgcaatcaacgaaatcgaatatgaaaacatcagcacggtggaaatcgacaaaggtgccggttcgtccgatcacggcagcggcgcactcggcggcgcggtcgccttccgcaccaaagaggcggcagacctgatttcagacggcaaaagttgggggatacaggcaaaaaccgcctacggcagtaaaaaccgccaatttatgaagtcgctcggcgcggggttcagcaaagacggttgggaagggctgttaatccgaaccgaacgccaagggcgggaaacgcgcccgcacggcgatattgcggacggggtggaatacggcatagaccgtttggacgcgttccgtcagacatacgatattaaacgcaagacaagagagccatttttctcagtagagggcgagcgtgaatccaagcccgtggcaaaattggcgggctacgggaaatatttgaacaaccagctcaaccgctgggtaaaagaacgtattgaacaaaatcagcctttaagtgctgaagaagaggcgcaggtgcgggaggcgcaggcgcgccacgaaaatctgtccgcccaagcttacacgggcggcggcaggatattgcccgatccgatggattaccgcagcggctcttggcttgccaagctgggctaccgcttcggcggcaggcattatgtcggcggcgtgtttgaggataccaaacagcgttacgatatccgcgatatgacggaaaaacagtattacggtacggacgaggcggaaaagtttagagacaagagcggggtgtacgacggcgacgatttccgcgacggcttgtattttgtgccgaatatagaagagtggaagggcgataaaaatttggtcaggggcataggtttgaaatattcccgcaccaaatttattgacgaacatcaccgccgccgccgtatgggtttgctgtatcgttatgaaaacgaagcgtattctgacaattgggcggataaggcggtgttgtcgtttgacaaacagggcgtggcaaccgataacaacacgctgaagctgaattgcgccgtgtatcctgctgtggacaaatcctgccgcgcgtcggcggacaaaccgtattcctacgacagcagcgaccgtttccactaccgcgaacagcacaatgttttgaatgcctcgtttgagaaatcgctgaaaaacaaatggacgaaacaccatctgactttgggcttcggttacgatgcttccaaagcgatttcccgccccgaacagctttcccacaatgcggcaaggatttcggaatccacgggattcgatgaaaacaatcaagataagtatcttttgggtaagcccgaagtcgtcgaagggtcggtctgcggctacatcgaaaccctgcgttcccgcaaatgcgtgccaagaaaaatcaacggcagcaatatccatatttctttgaacgaccgtttttcaatcggcaaatatttcgatttcagcttgggcggcaggtacgaccggaaaaacttcaccacgtcggaagaactcgtccgcagcgggcggtatgttgaccgttcgtggaacagcggcatcttgttcaaaccgaaccggcatttttccgtgtcttaccgtgcctccagcggcttcagaacgccctcattccaagaacttttcgggatagacatttatcacgattatccgaaaggctggcagcgtcccgccctgaaatcggaaaaggcagccaaccgggaaatcggtttgcagtggaagggcgatttcggctttttggaaatcagcagcttccgcaaccgttataccgatatgattgccgttgccgatcacaaaaccaaattgccgaatcaggcaggacaattgacagagattgatatacgcgattattacaatgcccaaaatatgtcgcttcaaggcgttaatatattgggaaaaatcgactggaacggcgtgtatggcaaactgcccgaaggtttgtacaccacattggcgtacaaccgcatcaaaccgaaatcggtatccaaccggccgggactgtccctccgcagctatgctttggatgcggtacagccgtcgcgttatgttttggggttcggatacgaccagcctgaggggaaatggggcgcaaacattatgctgacctattccaaagggaaaaaccctgacgagcttgcttatctggcaggcgatcaaaaacgatattcgacaaaaagagcgtcgtcttcttggtcgacggcagacgtttccgcctatctgaatctgaaaaaacggctgaccttgagggcggctatctacaatatcggcaactaccgctacgttacttgggaatccttgcgccagactgcggaaagcacggcaaaccggcacggcggcgacagcaactatggaaggtatgccgcaccgggcaggaacttcagtctcgcgctcgaaatgaagttttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z