BBa_K1590009 1 BBa_K1590009 Spermidine/putrescine-binding periplasmic protein 2015-09-08T11:00:00Z 2015-09-18T04:09:09Z PCR amplification of Escherichia Coli MG1655 gDNA. Escherichia coli PotD sequence, encoding Spermidine/putrescine-binding periplasmic protein. false false _2007_ 8083 24715 9 false Adding biobrick prefix, suffix, and double TAA stop codon to it via primers. Checked for forbidden restriction sites. Primers for amplification of PotD from E. coli MG1655 gDNA and subsequent insertion into pSB1C3. Forward: GCGC GAATTCGCGGCCGCTTCTAG A TGAAAAAATGGTCACGCCAC Reverse: GCGC CTGCAGCGGCCGCTACTAGT ATTATT AACGTCCTGCTTTCAGCTT Primers for cloning PotD into overexpression vector pQE80-L. Forward: GCGC AGATCTAAAAAATGGTCACGCCAC Reverse: GCGC GGTACCTTAACGTCCTGCTTTCAGCTTC false Manuel Blank annotation2447650 1 ATG start codon range2447650 1 1 3 annotation2447653 1 TAA stop codon range2447653 1 1045 1047 annotation2447652 1 PotD Protein Coding Sequence range2447652 1 1 1044 BBa_K1590009_sequence 1 atgaaaaaatggtcacgccacctgctcgcggcgggtgctctggcactgggcatgagcgccgctcacgccgatgacaacaacacgctgtatttctacaactggaccgagtacgtgccgccaggactgcttgaacagttcaccaaagaaaccggtattaaggttatctattcgacttacgagtcgaacgaaaccatgtacgccaagctgaaaacatacaaagacggtgcctatgatctggtggttccttcaacctattacgttgataaaatgcgtaaagaagggatgatccagaagatcgacaagtcgaagttaacaaacttcagcaatctcgatccagacatgctcaacaagccttttgacccgaataacgactactccattccgtatatctggggtgcgacggcgattggtgttaacggtgatgcggtggatccgaaatctgtcaccagctgggccgatctgtggaagccagagtacaaaggcagcctgctgttgaccgacgatgcccgtgaagtgttccagatggcgctgcgtaagctgggctactccggtaacaccaccgatccgaaagagattgaagctgcatataacgagctgaaaaaactgatgccaaacgtggcagcctttaactccgataacccggcgaacccgtacatggaaggcgaagttaacctcggcatgatctggaacggttctgctttcgttgcacgccaggcgggtacgccaattgacgtggtgtggccgaaagaaggcggcattttctggatggacagcctggcgatcccggcaaatgccaaaaacaaagaaggcgcgctgaaattgatcaacttcctgctgcgcccggatgtggcaaaacaggttgctgaaactatcggttatccaacgccaaaccttgcggcgcgtaagctgttaagtccagaagtggcgaacgataaaacactctacccggatgctgaaaccattaaaaatggcgaatggcagaatgacgttggcgcagccagcagcatttatgaagagtattatcagaagctgaaagcaggacgttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z