BBa_K1590010 1 BBa_K1590010 Murine Spermine Binding Protein 2015-09-08T11:00:00Z 2015-09-18T04:13:23Z In silico optimised version for E. coli K12 strains. Sequence ordered through IDT. Binds the carbohydrate spermine. false false _2007_ 8083 24715 9 false Choosing the correct codon-bias, remove 'illegal' restriction sites, ensure that (stop)codons are in frame, addition of standard prefix and suffix. Primers for cloning Spermine Binding Protein into overexpression vector pQE80-L. Forward: GCGC GGATCC CTGCTGCTGCTGACCCTGGCG Reverse: GCGC GGTACC TTAAGATTCGTCCTTCTGATC Part was synthesised with appropriate prefix/suffix sequences, hence no separate primers were required for cloning it into pSB1C3. false Manuel Blank annotation2447658 1 TAA stop codon range2447658 1 598 600 annotation2447656 1 ATG start codon range2447656 1 1 3 annotation2447657 1 Murine Spermine Binding Protein Protein Coding Sequence range2447657 1 1 597 BBa_K1590010_sequence 1 atgctgctgctgctgaccctggcgttcctggcgtctccgacctgccgtgcgcagaacgttctgggtaacgcggcgggtaaatacttctacgttcagggtgaagaccagggtcagctgaaaggtatgcgtatcttcctgtctgttttcaaattcatcaaaggtttccagctccagttcggttctaactggaccgacgtttacggcacccgttctgacaacttcatcgacttcctgctggaagacggtgaacacgttatcaaagttgaaggttctgcggttatctgcctgacctctctgaccttcaccaccaacaaaggtcgtgttgcgaccttcggtgttcgtcgtggtcgttacttctctgacaccggtggttctgacaaacacctggttaccgttaacggtatgcacgcgccgggtctgtgcgttcgtggtatcggtttcaaatggggtaacatcaacgcgaacggtaacgaccactacaacaacaaagaagacaaagcggacaacaaagacgcggacaacaaagacgctgacaataaagacgatggcgatgaggatgatgatggaaacgatgacgatgatcagaaggacgaatcttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z