BBa_K1592003 1 BBa_K1592003 Mcfp3 with LIP2 prepro 2015-09-05T11:00:00Z 2015-09-18T10:05:23Z Synthesized by IDT, Mcfp-3 is foot protein secreted from Mytilus californianus. The protein is of significance to the formation of byssus to help mussels permanently or temporarily tether to the surface of solid surface of reef or ship-body. This coherent substance shows the excellent adhesion performance with no substitute under water for sustaining the repeating wash of waves and having no toxicity. The signal tag, LIP2 prepro(BBa_K1592000), fused to Mcfp3 can lead the co-translational translocation of heterologous protein Mcfp-3 to be secreted out of the cell to achieve its function, during the progress of which the peptide will be deleted in the Golgi apparatus to eliminate the interference to the secreting protein. And hexahis-tag added between LIP2 prepro and Mcfp3 can therefore be used for protein purification. false false _2009_ 20267 20267 9 true none false Shuyan Tang annotation2444642 1 Mcfp3 range2444642 1 124 357 annotation2444643 1 BBa_K1592001 range2444643 1 124 357 annotation2444640 1 his-tag range2444640 1 106 123 annotation2444639 1 BamHI range2444639 1 100 105 annotation2444637 1 BBa_K1592000 range2444637 1 1 99 annotation2444638 1 LIP2 prepro range2444638 1 1 99 BBa_K1592003_sequence 1 atgaagctttccaccatccttttcacagcctgcgctaccctggctgccgccctcccttcccccatcactccttctgaggccgcagttctccagaagcgaggatcccaccaccaccaccaccacatgaataaattcagtgtcacagttttgctggctttagtccttattggattttttgccgttcagagtgacgcaggttatggttatgatctaggatataatgcaccatggccatacaacaatggttactatggctataatggatacaatggatatcacggacgttatggctggaataaaggctggaataacggtccatggggaggatcatattatggaaacaaaggctatttgtat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z