BBa_K1592004
1
Php4d
promoter hp4d
2015-08-31T11:00:00Z
2015-09-18T09:25:27Z
From our lab.
The recombinant promotor hp4d, nearly unaffected by pH, carbon source, nitrogen source and some conditions, can strongly promote gene expression in any culture medium(Madzak et al., 2000). Promoter hp4d is composed of four tandem repeated UAS1 sequences in upstream and the small LEU2 promoter, which is the sequence between TATA box and start condon ATG of LEU2 promoter in downstream. UAS1 is the upsteam activating sequence of the pXPR2, which is less affected by other conditions. Though any inducers are non-essential for activiation of php4d during cultivating, hp4d, activated by unknown element, isn't constitutive promoter.
The gene promoted by promoter hp4d usually expresses at the early stage of stabilization, called growth phase dependent (Nicaud et al., 2002). This feature, separating the strain growth and producing foreign protein, is useful for production of foreign protein, which avoids the harm by expressing foreign protein.
false
false
_2009_
20267
20267
9
false
No.
false
Shuyan Tang
annotation2441400
1
LEU
range2441400
1
477
550
annotation2441396
1
UAS1-2
range2441396
1
133
241
annotation2441399
1
UAS1-4
range2441399
1
351
453
annotation2441398
1
UAS1-3
range2441398
1
242
350
annotation2441397
1
UAS1-1
range2441397
1
24
132
BBa_K1592004_sequence
1
atcgatacgcgtgcatgctgaggtgtctcacaagtgccgtgcagtcccgcccccacttgcttctctttgtgtgtagtgtacgtacattatcgagaccgttgttcccgcccacctcgatccggcatgctgaggtgtctcacaagtgccgtgcagtcccgcccccacttgcttctctttgtgtgtagtgtacgtacattatcgagaccgttgttcccgcccacctcgatccggcatgctgaggtgtctcacaagtgccgtgcagtcccgcccccacttgcttctctttgtgtgtagtgtacgtacattatcgagaccgttgttcccgcccacctcgatccggcatgctgaggtgtctcacaagtgccgtgcagtcccgcccccacttgcttctctttgtgtgtagtgtacgtacattatcgagaccgttgttcccgcccacctcgatccggcatgcactgatcacgggcaaaagtgcgtatatatacaagagcgtttgccagccacagattttcactccacacaccacatcacacatacaaccacacacatccaca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z