BBa_K1592022 1 BBa_K1592022 ptrp mutant3 2015-09-08T11:00:00Z 2015-09-09T08:32:05Z none ptrp is a promoter with the trp operator. It will be repressed by trpR (K588000) and LovTAP. Note that the original trp operator contains two SpeI sites。 We remove the illegal sites without affecting its function by site-directed mutagenesis for meeting the requirements of RFC10 and more convenient to be assembled, and finally constructed three ptrp muant, called ptrp mutant1, ptrp mutant2, and ptrp mutant3. false false _2009_ 20267 20267 9 false none false Shuyan Tang annotation2456102 1 T-C range2456102 1 48 48 annotation2456103 1 A-C range2456103 1 54 54 BBa_K1592022_sequence 1 tggcaaatattctgaaatgagctgttgacaattaatcatcgaactagctaactcgtacgcaagttcacgtaaaaagggtatcgac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z