LOCUS       BBa_K1593000            1819 bp    DNA     linear       25-Nov-2024
DEFINITION  lac-compx with RFP
ACCESSION   BBa_K1593000
VERSION     BBa_K1593000.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..901
                     /label=mrfp1
     misc_feature    908..932
                     /label=Help:Barcodes
     CDS             227..907
                     /label=BBa_E1010
     stem_loop       952..995
                     /label=stem_loop
     misc_feature    941..1020
                     /label=BBa_B0010
     terminator      941..1020
                     /label=BBa_B0010
     stem_loop       1036..1055
                     /label=T7 TE
     misc_feature    1062..1062
                     /label=stop
     polyA_site      1056..1069
                     /label=polya
     misc_feature    1029..1069
                     /label=BBa_B0012
     terminator      1029..1069
                     /label=BBa_B0012
     misc_feature    1..1069
                     /label=BBa_J04450
     CDS             1078..1165
                     /label=end of LacI coding region (inactive)
     misc_feature    1078..1277
                     /label=BBa_R0010
     misc_feature    1166..1203
                     /label=CAP binding site
     promoter        1214..1219
                     /label=-35
     promoter        1238..1243
                     /label=-10
     misc_feature    1243..1277
                     /label=LacI binding site
     misc_feature    1250..1250
                     /label=start
     promoter        1078..1277
                     /label=BBa_R0010
     misc_feature    1290..1293
                     /label=conserved
     RBS             1286..1297
                     /label=BBa_B0034
     misc_feature    1304..1819
                     /label=BBa_K1593001
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
      781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
      841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
      901 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga
      961 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1021 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagcaa
     1081 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     1141 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     1201 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     1261 gataacaatt tcacacatac tagagaaaga ggagaaatac tagatgaaaa agcttgcatg
     1321 tctttcagca ctggccgcag ttctggcttt caccgcaggt acttccgtag ctgcgacttc
     1381 tactgtaact ggcggttacg cacagagcga cgctcagggc caaatgaaca aaatgggcgg
     1441 tttcaacctg aaataccgct atgaagaaga caacagcccg ctgggtgtga tcggttcttt
     1501 cacttacacc gagaaaagcc gtactgcaag ctctggtgac tacaacaaaa accagtacta
     1561 cggcatcact gctggtccgg cttaccgcat taacgactgg gcaagcatct acggtgtagt
     1621 gggtgtgggt tatggtaaat tccagaccac tgaatacccg acctacaaac acgacaccag
     1681 cgactacggt ttctcctacg gtgcgggtct gcagttcaac ccgatggaaa acgttgctct
     1741 ggacttctct tacgagcaga gccgtattcg tagcgttgac gtaggcacct ggattgccgg
     1801 tgttggttac cgcttctaa
//