BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1223014 1 BBa_K1223014 tRNA-Pyl (pylT) gene from Methanosarcina barkeri str. Fusaro 2013-10-02T11:00:00Z 2015-05-08T01:09:43Z Methanosarcina barkeri str. Fusaro genomic sequence. this is the DNA coding sequence for the tRNA of pyrrolysine from the Archaea Methanosarcina barkeri str. Fusaro. this tRNA is a part of the machinery that is used to incorporate pyrolysine into proteins in E.coli. the tRNA synthetase that charges the tRNA with pyrrolysine can be found in biobrick BBa_k1223013. the tRNA has the anticodon CUA - which means that the sense codon needed to incorporate pyrrolysine is TAG - the amber stop codon. false false _1537_ 0 18143 9 It's complicated true this is a part of the pyrrolysine incorporation machinery. false Orr Schlesinger annotation2367519 1 CUA anticodon range2367519 1 31 33 annotation2367517 1 pyrrolysine tRNA CDS range2367517 1 1 72 BBa_I712074 1 BBa_I712074 T7 promoter (strong promoter from T7 bacteriophage) 2007-10-21T11:00:00Z 2015-08-31T04:07:46Z T7 bacteriophage T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes. false false _130_ 0 1856 9 In stock false true Rok Gaber BBa_K1593002 1 BBa_K1593002 tRNA system 2015-08-17T11:00:00Z 2015-08-18T06:05:08Z parts tRNA false false _2010_ 27863 27863 9 It's complicated false null false Zeyu Shen component2436096 1 BBa_I712074 component2436098 1 BBa_B0034 component2436092 1 BBa_I712074 component2436095 1 BBa_K1223014 component2436099 1 BBa_K1492002 annotation2436098 1 BBa_B0034 range2436098 1 189 200 annotation2436096 1 BBa_I712074 range2436096 1 135 180 annotation2436095 1 BBa_K1223014 range2436095 1 55 126 annotation2436099 1 BBa_K1492002 range2436099 1 207 1127 annotation2436092 1 BBa_I712074 range2436092 1 1 46 BBa_K1492002 1 Mj TyrRS Methanococcus jannaschii tyrosyl-tRNA synthetase 2014-07-27T11:00:00Z 2015-05-08T01:10:44Z - Pyrrolysyl-tRNA Synthetase introduces the Non Natural Amino acid p-Azido-L-Phenylalanine at the TAG codon. false false _1872_ 0 21091 9 In stock false - false Wiggert Altenburg BBa_B0034_sequence 1 aaagaggagaaa BBa_K1223014_sequence 1 gggaacctgatcatgtagatcgaatggactctaaatccgttcagccgggttagattcccggggtttccgcca BBa_I712074_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgca BBa_K1593002_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgcatactagaggggaacctgatcatgtagatcgaatggactctaaatccgttcagccgggttagattcccggggtttccgccatactagagtaatacgactcactatagggaatacaagctacttgttctttttgcatactagagaaagaggagaaatactagatggacgagttcgaaatgattaaacgcaacaccagcgaaattatctctgaagaagagctgcgcgaggtgctgaagaaagacgagaagagcgcgactattggctttgagccgtccggtaaaattcacctgggtcactacctgcaaatcaagaagatgattgatctgcaaaacgctggttttgacatcattatcctgctggcggacctgcacgcctacctgaatcaaaagggcgagctggatgagattcgcaagatcggcgactacaataagaaagtcttcgaagccatgggtttgaaggctaaatacgtctacggtagcaattttcagctggataaggattacacgttgaatgtgtaccgtctggcgctgaaaaccacgctgaaacgcgcccgtcgttccatggagctgattgcgcgcgaggatgagaatccaaaagttgctgaggttatttaccctattatgcaagttaatccgttgcactaccagggtgttgatgttgccgtcggtggtatggagcaacgcaaaattcacatgctggcacgtgaactgctgccgaaaaaggttgtctgtattcataatccggtcctgaccggcctggatggcgagggtaaaatgagcagcagcaagggtaactttattgcagttgacgatagcccggaagaaatccgtgcgaagatcaagaaagcgtactgcccggcaggcgtggttgagggtaacccgatcatggaaatcgccaagtattttctggaatacccactgacgattaagcgcccggagaaatttggcggcgacctgaccgtcaacagctacgaggagctggaaagcttgtttaagaacaaagaactgcatccgatgcgcctgaaaaacgccgtggcggaagagctgattaagattctggaaccaattcgcaaacgtctgtaa BBa_K1492002_sequence 1 atggacgagttcgaaatgattaaacgcaacaccagcgaaattatctctgaagaagagctgcgcgaggtgctgaagaaagacgagaagagcgcgactattggctttgagccgtccggtaaaattcacctgggtcactacctgcaaatcaagaagatgattgatctgcaaaacgctggttttgacatcattatcctgctggcggacctgcacgcctacctgaatcaaaagggcgagctggatgagattcgcaagatcggcgactacaataagaaagtcttcgaagccatgggtttgaaggctaaatacgtctacggtagcaattttcagctggataaggattacacgttgaatgtgtaccgtctggcgctgaaaaccacgctgaaacgcgcccgtcgttccatggagctgattgcgcgcgaggatgagaatccaaaagttgctgaggttatttaccctattatgcaagttaatccgttgcactaccagggtgttgatgttgccgtcggtggtatggagcaacgcaaaattcacatgctggcacgtgaactgctgccgaaaaaggttgtctgtattcataatccggtcctgaccggcctggatggcgagggtaaaatgagcagcagcaagggtaactttattgcagttgacgatagcccggaagaaatccgtgcgaagatcaagaaagcgtactgcccggcaggcgtggttgagggtaacccgatcatggaaatcgccaagtattttctggaatacccactgacgattaagcgcccggagaaatttggcggcgacctgaccgtcaacagctacgaggagctggaaagcttgtttaagaacaaagaactgcatccgatgcgcctgaaaaacgccgtggcggaagagctgattaagattctggaaccaattcgcaaacgtctgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z