BBa_K1223014
1
BBa_K1223014
tRNA-Pyl (pylT) gene from Methanosarcina barkeri str. Fusaro
2013-10-02T11:00:00Z
2015-05-08T01:09:43Z
Methanosarcina barkeri str. Fusaro genomic sequence.
this is the DNA coding sequence for the tRNA of pyrrolysine from the Archaea Methanosarcina barkeri str. Fusaro.
this tRNA is a part of the machinery that is used to incorporate pyrolysine into proteins in E.coli.
the tRNA synthetase that charges the tRNA with pyrrolysine can be found in biobrick BBa_k1223013.
the tRNA has the anticodon CUA - which means that the sense codon needed to incorporate pyrrolysine is TAG - the amber stop codon.
false
false
_1537_
0
18143
9
It's complicated
true
this is a part of the pyrrolysine incorporation machinery.
false
Orr Schlesinger
annotation2367519
1
CUA anticodon
range2367519
1
31
33
annotation2367517
1
pyrrolysine tRNA CDS
range2367517
1
1
72
BBa_K1593888
1
BBa_K1593888
tRNA +tRNA synthetase
2015-09-17T11:00:00Z
2015-09-18T12:26:26Z
it comes from bacterial
use this part to synthetase tRNA
false
false
_2010_
27877
27877
9
false
use this to introduce a mutation
false
Yuting Liu
component2473494
1
BBa_K1223014
component2473495
1
BBa_K1492002
annotation2473495
1
BBa_K1492002
range2473495
1
79
999
annotation2473494
1
BBa_K1223014
range2473494
1
1
72
BBa_K1492002
1
Mj TyrRS
Methanococcus jannaschii tyrosyl-tRNA synthetase
2014-07-27T11:00:00Z
2015-05-08T01:10:44Z
-
Pyrrolysyl-tRNA Synthetase introduces the Non Natural Amino acid p-Azido-L-Phenylalanine at the TAG codon.
false
false
_1872_
0
21091
9
In stock
false
-
false
Wiggert Altenburg
BBa_K1223014_sequence
1
gggaacctgatcatgtagatcgaatggactctaaatccgttcagccgggttagattcccggggtttccgcca
BBa_K1593888_sequence
1
gggaacctgatcatgtagatcgaatggactctaaatccgttcagccgggttagattcccggggtttccgccatactagatggacgagttcgaaatgattaaacgcaacaccagcgaaattatctctgaagaagagctgcgcgaggtgctgaagaaagacgagaagagcgcgactattggctttgagccgtccggtaaaattcacctgggtcactacctgcaaatcaagaagatgattgatctgcaaaacgctggttttgacatcattatcctgctggcggacctgcacgcctacctgaatcaaaagggcgagctggatgagattcgcaagatcggcgactacaataagaaagtcttcgaagccatgggtttgaaggctaaatacgtctacggtagcaattttcagctggataaggattacacgttgaatgtgtaccgtctggcgctgaaaaccacgctgaaacgcgcccgtcgttccatggagctgattgcgcgcgaggatgagaatccaaaagttgctgaggttatttaccctattatgcaagttaatccgttgcactaccagggtgttgatgttgccgtcggtggtatggagcaacgcaaaattcacatgctggcacgtgaactgctgccgaaaaaggttgtctgtattcataatccggtcctgaccggcctggatggcgagggtaaaatgagcagcagcaagggtaactttattgcagttgacgatagcccggaagaaatccgtgcgaagatcaagaaagcgtactgcccggcaggcgtggttgagggtaacccgatcatggaaatcgccaagtattttctggaatacccactgacgattaagcgcccggagaaatttggcggcgacctgaccgtcaacagctacgaggagctggaaagcttgtttaagaacaaagaactgcatccgatgcgcctgaaaaacgccgtggcggaagagctgattaagattctggaaccaattcgcaaacgtctgtaa
BBa_K1492002_sequence
1
atggacgagttcgaaatgattaaacgcaacaccagcgaaattatctctgaagaagagctgcgcgaggtgctgaagaaagacgagaagagcgcgactattggctttgagccgtccggtaaaattcacctgggtcactacctgcaaatcaagaagatgattgatctgcaaaacgctggttttgacatcattatcctgctggcggacctgcacgcctacctgaatcaaaagggcgagctggatgagattcgcaagatcggcgactacaataagaaagtcttcgaagccatgggtttgaaggctaaatacgtctacggtagcaattttcagctggataaggattacacgttgaatgtgtaccgtctggcgctgaaaaccacgctgaaacgcgcccgtcgttccatggagctgattgcgcgcgaggatgagaatccaaaagttgctgaggttatttaccctattatgcaagttaatccgttgcactaccagggtgttgatgttgccgtcggtggtatggagcaacgcaaaattcacatgctggcacgtgaactgctgccgaaaaaggttgtctgtattcataatccggtcctgaccggcctggatggcgagggtaaaatgagcagcagcaagggtaactttattgcagttgacgatagcccggaagaaatccgtgcgaagatcaagaaagcgtactgcccggcaggcgtggttgagggtaacccgatcatggaaatcgccaagtattttctggaatacccactgacgattaagcgcccggagaaatttggcggcgacctgaccgtcaacagctacgaggagctggaaagcttgtttaagaacaaagaactgcatccgatgcgcctgaaaaacgccgtggcggaagagctgattaagattctggaaccaattcgcaaacgtctgtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z