LOCUS       BBa_K1595020            1404 bp    DNA     linear       18-May-2024
DEFINITION  MBP with EcnB from Ralstonia pickettii.
ACCESSION   BBa_K1595020
VERSION     BBa_K1595020.1  
FEATURES             Location/Qualifiers
     promoter        2..20
                     /label=T7
     RBS             22..80
                     /label=RBS
     CDS             81..1193
                     /label=MBP
     misc_feature    1194..1214
                     /label=TEV cutsite
     CDS             1215..1343
                     /label=EcnB
     misc_feature    1344..1361
                     /label=6xHist tag
     misc_feature    1365..1403
                     /label=Terminator
ORIGIN
        1 gtaatacgac tcactatagg gaatacaagc tacttgttct ttttgcaggt acctactaga
       61 gaaagacagg accctactag atgaaaatcg aagaaggtaa actggtaatc tggattaacg
      121 gcgataaagg ctataacggt ctcgctgaag tcggtaagaa attcgagaaa gataccggaa
      181 ttaaagtcac cgttgagcat ccggataaac tggaagagaa attcccacag gttgcggcaa
      241 ctggcgatgg ccctgacatt atcttctggg cacacgaccg ctttggtggc tacgctcaat
      301 ctggcctgtt ggctgaaatc accccggaca aagcgttcca ggacaagctg tatccgttta
      361 cctgggatgc cgtacgttac aacggcaagc tgattgctta cccgatcgct gttgaagcgt
      421 tatcgctgat ttataacaaa gatctgctgc cgaacccgcc aaaaacctgg gaagagatcc
      481 cggcgctgga taaagaactg aaagcgaaag gtaagagcgc gctgatgttc aacctgcaag
      541 aaccgtactt cacctggccg ctgattgctg ctgacggggg ttatgcgttc aagtatgaaa
      601 acggcaagta cgacattaaa gacgtgggcg tggataacgc tggcgcgaaa gcgggtctga
      661 ccttcctggt tgacctgatt aaaaacaaac acatgaatgc agacaccgat tactccatcg
      721 cagaagctgc ctttaataaa ggcgaaacag cgatgaccat caacggcccg tgggcatggt
      781 ccaacatcga caccagcaaa gtgaattatg gtgtaacggt actgccgacc ttcaagggtc
      841 aaccatccaa accgttcgtt ggcgtgctga gcgcaggtat taacgccgcc agtccgaaca
      901 aagagctggc gaaagagttc ctcgaaaact atctgctgac tgatgaaggt ctggaagcgg
      961 ttaataaaga caaaccgctg ggtgccgtag cgctgaagtc ttacgaggaa gagttggcga
     1021 aagatccacg tattgccgcc accatggaaa acgcccagaa aggtgaaatc atgccgaaca
     1081 tcccgcagat gtccgctttc tggtatgccg tgcgtactgc ggtgatcaac gccgccagcg
     1141 gtcgtcagac tgtcgatgaa gccctgaaag acgcgcagac tcgtatcacc aaggaaaacc
     1201 tgtatttcca gggtatgaaa cgcaccctga ttgcgctgct ggcgtgcctg ggcatgctgc
     1261 agctggcggg ctgcaacacc atggcgggcc tgggcaaaga tacccaggcg gcgggcagca
     1321 gcctggaacg cgcggcgaaa aaacatcatc atcatcatca ttaaaaaaaa aaaccccgcc
     1381 cctgacaggg cggggttttt tttt
//