LOCUS       BBa_K1595023            1419 bp    DNA     linear       29-Apr-2024
DEFINITION  MBP with EcnB from Escherichia coli str. K-12 substr. DH10B
ACCESSION   BBa_K1595023
VERSION     BBa_K1595023.1  
FEATURES             Location/Qualifiers
     misc_feature    1380..1418
                     /label=Terminator
     misc_feature    1359..1376
                     /label=6xHist tag
     CDS             1215..1358
                     /label=EcnB
     misc_feature    1194..1214
                     /label=TEV cutsite
     CDS             81..1193
                     /label=MBP
     RBS             22..80
                     /label=RBS
     promoter        2..20
                     /label=T7
ORIGIN
        1 gtaatacgac tcactatagg gaatacaagc tacttgttct ttttgcaggt acctactaga
       61 gaaagacagg accctactag atgaaaatcg aagaaggtaa actggtaatc tggattaacg
      121 gcgataaagg ctataacggt ctcgctgaag tcggtaagaa attcgagaaa gataccggaa
      181 ttaaagtcac cgttgagcat ccggataaac tggaagagaa attcccacag gttgcggcaa
      241 ctggcgatgg ccctgacatt atcttctggg cacacgaccg ctttggtggc tacgctcaat
      301 ctggcctgtt ggctgaaatc accccggaca aagcgttcca ggacaagctg tatccgttta
      361 cctgggatgc cgtacgttac aacggcaagc tgattgctta cccgatcgct gttgaagcgt
      421 tatcgctgat ttataacaaa gatctgctgc cgaacccgcc aaaaacctgg gaagagatcc
      481 cggcgctgga taaagaactg aaagcgaaag gtaagagcgc gctgatgttc aacctgcaag
      541 aaccgtactt cacctggccg ctgattgctg ctgacggggg ttatgcgttc aagtatgaaa
      601 acggcaagta cgacattaaa gacgtgggcg tggataacgc tggcgcgaaa gcgggtctga
      661 ccttcctggt tgacctgatt aaaaacaaac acatgaatgc agacaccgat tactccatcg
      721 cagaagctgc ctttaataaa ggcgaaacag cgatgaccat caacggcccg tgggcatggt
      781 ccaacatcga caccagcaaa gtgaattatg gtgtaacggt actgccgacc ttcaagggtc
      841 aaccatccaa accgttcgtt ggcgtgctga gcgcaggtat taacgccgcc agtccgaaca
      901 aagagctggc gaaagagttc ctcgaaaact atctgctgac tgatgaaggt ctggaagcgg
      961 ttaataaaga caaaccgctg ggtgccgtag cgctgaagtc ttacgaggaa gagttggcga
     1021 aagatccacg tattgccgcc accatggaaa acgcccagaa aggtgaaatc atgccgaaca
     1081 tcccgcagat gtccgctttc tggtatgccg tgcgtactgc ggtgatcaac gccgccagcg
     1141 gtcgtcagac tgtcgatgaa gccctgaaag acgcgcagac tcgtatcacc aaggaaaacc
     1201 tgtatttcca gggtatggtg aaaaaaacca ttgcggcgat ttttagcgtg ctggtgctga
     1261 gcaccgtgct gaccgcgtgc aacaccaccc gcggcgtggg cgaagatatt agcgatggcg
     1321 gcaacgcgat tagcggcgcg gcgaccaaag cgcagcagca tcatcatcat catcattaaa
     1381 aaaaaaaacc ccgcccctga cagggcgggg ttttttttt
//