##gff-version 3 ##sequence-region BBa_K1595023 2 1418 BBa_K1595023 . CDS 1215 1358 . + 0 ID=annotation2453259;Name=EcnB BBa_K1595023 . promoter 2 20 . + 0 ID=annotation2453263;Name=T7 BBa_K1595023 . sequence_feature 1380 1418 . + 0 ID=annotation2453257;Name=Terminator BBa_K1595023 . sequence_feature 1194 1214 . + 0 ID=annotation2453260;Name=TEV cutsite BBa_K1595023 . tag 1359 1376 . + 0 ID=annotation2453258;Name=6xHist tag BBa_K1595023 . ribosome_entry_site 22 80 . + 0 ID=annotation2453262;Name=RBS BBa_K1595023 . CDS 81 1193 . + 0 ID=annotation2453261;Name=MBP >BBa_K1595023 gtaatacgactcactatagggaatacaagctacttgttctttttgcaggtacctactagagaaagacaggaccctacta gatgaaaatcgaagaaggtaaactggtaatctggattaacggcgataaaggctataacggtctcgctgaagtcggtaag aaattcgagaaagataccggaattaaagtcaccgttgagcatccggataaactggaagagaaattcccacaggttgcgg caactggcgatggccctgacattatcttctgggcacacgaccgctttggtggctacgctcaatctggcctgttggctga aatcaccccggacaaagcgttccaggacaagctgtatccgtttacctgggatgccgtacgttacaacggcaagctgatt gcttacccgatcgctgttgaagcgttatcgctgatttataacaaagatctgctgccgaacccgccaaaaacctgggaag agatcccggcgctggataaagaactgaaagcgaaaggtaagagcgcgctgatgttcaacctgcaagaaccgtacttcac ctggccgctgattgctgctgacgggggttatgcgttcaagtatgaaaacggcaagtacgacattaaagacgtgggcgtg gataacgctggcgcgaaagcgggtctgaccttcctggttgacctgattaaaaacaaacacatgaatgcagacaccgatt actccatcgcagaagctgcctttaataaaggcgaaacagcgatgaccatcaacggcccgtgggcatggtccaacatcga caccagcaaagtgaattatggtgtaacggtactgccgaccttcaagggtcaaccatccaaaccgttcgttggcgtgctg agcgcaggtattaacgccgccagtccgaacaaagagctggcgaaagagttcctcgaaaactatctgctgactgatgaag gtctggaagcggttaataaagacaaaccgctgggtgccgtagcgctgaagtcttacgaggaagagttggcgaaagatcc acgtattgccgccaccatggaaaacgcccagaaaggtgaaatcatgccgaacatcccgcagatgtccgctttctggtat gccgtgcgtactgcggtgatcaacgccgccagcggtcgtcagactgtcgatgaagccctgaaagacgcgcagactcgta tcaccaaggaaaacctgtatttccagggtatggtgaaaaaaaccattgcggcgatttttagcgtgctggtgctgagcac cgtgctgaccgcgtgcaacaccacccgcggcgtgggcgaagatattagcgatggcggcaacgcgattagcggcgcggcg accaaagcgcagcagcatcatcatcatcatcattaaaaaaaaaaaccccgcccctgacagggcggggttttttttt