LOCUS       BBa_K1595030            2031 bp    DNA     linear       27-Dec-2024
DEFINITION  EDA linked to cel5a via GSG linker
ACCESSION   BBa_K1595030
VERSION     BBa_K1595030.1  
FEATURES             Location/Qualifiers
     misc_feature    1989..2031
                     /label=Terminator
     misc_feature    1971..1988
                     /label=6xHist tag
     misc_feature    1965..1970
                     /label=BamHI cutsite
     CDS             732..1964
                     /label=Cel5a
     misc_feature    729..734
                     /label=NdeI cutsite
     CDS             720..728
                     /label=GSG linker
     CDS             81..719
                     /label=EDA
     RBS             22..80
                     /label=RBS
     promoter        1..21
                     /label=T7
ORIGIN
        1 gtaatacgac tcactatagg gaatacaagc tacttgttct ttttgcaggt acctactaga
       61 gaaagacagg accctactag atgaaaaact ggaaaaccag cgcggaaagc attctgacca
      121 ccggcccggt ggtgccggtg attgtggtga aaaaactgga acatgcggtg ccgatggcga
      181 aagcgctggt ggcgggcggc gtgcgcgtgc tggaagtgac cctgcgcacc gaatgcgcgg
      241 tggatgcgat tcgcgcgatt gcgaaagaag tgccggaagc gattgtgggc gcgggcaccg
      301 tgctgaaccc gcagcagctg gcggaagtga ccgaagcggg cgcgcagttt gcgattagcc
      361 cgggcctgac cgaaccgctg ctgaaagcgg cgaccgaagg caccattccg ctgattccgg
      421 gcattagcac cgtgagcgaa ctgatgctgg gcatggatta tggcctgaaa gaatttaaat
      481 tttttccggc ggaagcgaac ggcggcgtga aagcgttaca ggcgattgcg ggcccgttta
      541 gccaggtgcg cttttgcccg accggcggca ttagcccggc gaactatcgc gattatctgg
      601 cgctgaaaag cgtgctgtgc attggcggca gctggctggt gccggcggat gcgctggaag
      661 cgggcgatta tgatcgcatt accaaactgg cgcgcgaagc ggtggaaggc gcgaaactgg
      721 gaagcggaca tatgagatcc tcacataagc gtaatttggc tgcgctgctt tccatcagcg
      781 gagccgcggt ggcacagcag acggcatggg gtcaatgcgg aggacaagga tggtccggtg
      841 cgacatcatg tgtgtcaggc tacacttgct cctacacaaa tgactggtac tcccagtgcg
      901 ttcctggatc atccggcggc ggaggtagca accctacaac gaccaccacg atgatccgga
      961 cgaccacgac ggcttcctcc tcgggcccca ccggcggcgc tggtggtggt aaggttcgat
     1021 ttgccggtgt caacatcgcc ggctttgatt tcggagtggt gatcagcggc actcaagaca
     1081 tgacccaaat tgtggacgag tccgtcgatg gtgtcaatca gatgcgacac tttgtcaacg
     1141 acgacggctt caacatcttc cgcctcccgt caggctggca gttcctggtg aacaacaacc
     1201 tcggtggctc actggactcg aacaatttcg ccaagtacga caagctggtt caaggatgtc
     1261 tgtccctggg agcatactgt atcgtcgatg tccacaatta cgctcgctgg aacggcggcg
     1321 tcatcggaca aggcggtccc accgacgacc agttcaccag tctctggact caactggcca
     1381 ctcactacaa gagtgaaagc aagatcatct ttggtgtgat gaacgagcca cacgacctgg
     1441 acatcaaccg atgggccacc accgtgcaaa aggccgtgac ggcgatccgc aaggccggcg
     1501 ccacttccca aatgatcctt cttccaggta ccgacttcac cagtgcggcc aactttgtcg
     1561 agaatggctc cggtgccgcg ctctcggccg tcaccaacct ggacgggtcc accaccaacc
     1621 tgatcttcga cgtgcacaag tatctcgact ccgataacag cggcacccac gccgagtgcg
     1681 tgaccaacaa cgccgacgct ttcaacagcc tggcccaatg gctccgcacc aacaagcgtc
     1741 aagccatgct caccgagacc ggtggtggta acgtccagag ctgcggaaca tacatgtgcc
     1801 agcaactgga tgtgctcaac caaaactctg acgtttacct tggatggacc tcgtggagcg
     1861 ctggaggttt ccaggtttcc tggaactatg tgctgggtga ggtcccaacc aacaacgttg
     1921 atacctacct ggtcaagcaa tgctttgttc ccaagtggaa gaacggatcc catcatcatc
     1981 atcatcatta aaaaaaaaaa ccccgcccct gacagggcgg ggtttttttt t
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