BBa_K1595030 1 BBa_K1595030 EDA linked to cel5a via GSG linker 2015-09-12T11:00:00Z 2015-09-18T01:47:12Z Synthesize by DNA 2.0 Coding device for KDPG aldolase (EDA), a peptide-stabilizing protein, with a GSG linker and protein cel5a. false false _2012_ 18839 18839 9 false Use in conjunction with strain-expressing T7 polymerase. cel5a is a stable, detectable protein that can be used to determine expression of our plasmid. false Jonlin Chen annotation2453656 1 GSG linker range2453656 1 720 728 annotation2453652 1 6xHist tag range2453652 1 1971 1988 annotation2453659 1 T7 range2453659 1 1 21 annotation2453657 1 EDA range2453657 1 81 719 annotation2453655 1 NdeI cutsite range2453655 1 729 734 annotation2453658 1 RBS range2453658 1 22 80 annotation2453651 1 Terminator range2453651 1 1989 2031 annotation2453653 1 BamHI cutsite range2453653 1 1965 1970 annotation2453654 1 Cel5a range2453654 1 732 1964 BBa_K1595030_sequence 1 gtaatacgactcactatagggaatacaagctacttgttctttttgcaggtacctactagagaaagacaggaccctactagatgaaaaactggaaaaccagcgcggaaagcattctgaccaccggcccggtggtgccggtgattgtggtgaaaaaactggaacatgcggtgccgatggcgaaagcgctggtggcgggcggcgtgcgcgtgctggaagtgaccctgcgcaccgaatgcgcggtggatgcgattcgcgcgattgcgaaagaagtgccggaagcgattgtgggcgcgggcaccgtgctgaacccgcagcagctggcggaagtgaccgaagcgggcgcgcagtttgcgattagcccgggcctgaccgaaccgctgctgaaagcggcgaccgaaggcaccattccgctgattccgggcattagcaccgtgagcgaactgatgctgggcatggattatggcctgaaagaatttaaattttttccggcggaagcgaacggcggcgtgaaagcgttacaggcgattgcgggcccgtttagccaggtgcgcttttgcccgaccggcggcattagcccggcgaactatcgcgattatctggcgctgaaaagcgtgctgtgcattggcggcagctggctggtgccggcggatgcgctggaagcgggcgattatgatcgcattaccaaactggcgcgcgaagcggtggaaggcgcgaaactgggaagcggacatatgagatcctcacataagcgtaatttggctgcgctgctttccatcagcggagccgcggtggcacagcagacggcatggggtcaatgcggaggacaaggatggtccggtgcgacatcatgtgtgtcaggctacacttgctcctacacaaatgactggtactcccagtgcgttcctggatcatccggcggcggaggtagcaaccctacaacgaccaccacgatgatccggacgaccacgacggcttcctcctcgggccccaccggcggcgctggtggtggtaaggttcgatttgccggtgtcaacatcgccggctttgatttcggagtggtgatcagcggcactcaagacatgacccaaattgtggacgagtccgtcgatggtgtcaatcagatgcgacactttgtcaacgacgacggcttcaacatcttccgcctcccgtcaggctggcagttcctggtgaacaacaacctcggtggctcactggactcgaacaatttcgccaagtacgacaagctggttcaaggatgtctgtccctgggagcatactgtatcgtcgatgtccacaattacgctcgctggaacggcggcgtcatcggacaaggcggtcccaccgacgaccagttcaccagtctctggactcaactggccactcactacaagagtgaaagcaagatcatctttggtgtgatgaacgagccacacgacctggacatcaaccgatgggccaccaccgtgcaaaaggccgtgacggcgatccgcaaggccggcgccacttcccaaatgatccttcttccaggtaccgacttcaccagtgcggccaactttgtcgagaatggctccggtgccgcgctctcggccgtcaccaacctggacgggtccaccaccaacctgatcttcgacgtgcacaagtatctcgactccgataacagcggcacccacgccgagtgcgtgaccaacaacgccgacgctttcaacagcctggcccaatggctccgcaccaacaagcgtcaagccatgctcaccgagaccggtggtggtaacgtccagagctgcggaacatacatgtgccagcaactggatgtgctcaaccaaaactctgacgtttaccttggatggacctcgtggagcgctggaggtttccaggtttcctggaactatgtgctgggtgaggtcccaaccaacaacgttgatacctacctggtcaagcaatgctttgttcccaagtggaagaacggatcccatcatcatcatcatcattaaaaaaaaaaaccccgcccctgacagggcggggttttttttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z