LOCUS       BBa_K1595031             438 bp    DNA     linear       05-May-2024
DEFINITION  EcnA and EcnB from Escherichia coli str. K-12 substr. DH10B
ACCESSION   BBa_K1595031
VERSION     BBa_K1595031.1  
FEATURES             Location/Qualifiers
     misc_feature    396..438
                     /label=Terminator
     misc_feature    390..395
                     /label=BamHI cutsite
     misc_feature    372..389
                     /label=6xHist tag
     CDS             228..371
                     /label=EcnB
     misc_feature    207..227
                     /label=TEV cutsite
     CDS             87..206
                     /label=EcnA
     misc_feature    81..86
                     /label=NdeI cutsite
     RBS             22..80
                     /label=RBS
     promoter        1..21
                     /label=T7
ORIGIN
        1 gtaatacgac tcactatagg gaatacaagc tacttgttct ttttgcaggt acctactaga
       61 gaaagacagg accctactag catatgatga aacgcctgat tgtgctggtg ctgctggcga
      121 gcaccctgct gaccggctgc aacaccgcgc gcggctttgg cgaagatatt aaacatctgg
      181 gcaacagcat tagccgcgcg gcgagcgaaa acctgtattt ccagggtatg gtgaaaaaaa
      241 ccattgcggc gatttttagc gtgctggtgc tgagcaccgt gctgaccgcg tgcaacacca
      301 cccgcggcgt gggcgaagat attagcgatg gcggcaacgc gattagcggc gcggcgacca
      361 aagcgcagca gcatcatcat catcatcatg gatcctaaaa aaaaaaaccc cgcccctgac
      421 agggcggggt tttttttt
//