BBa_K1595031
1
BBa_K1595031
EcnA and EcnB from Escherichia coli str. K-12 substr. DH10B
2015-09-17T11:00:00Z
2016-02-03T10:54:52Z
Synthesized by DNA 2.0
T7 + RBS + EDA + GSG + NdeI + EcnA + TEV + EcnB + 6xHist tag + BamHI + Terminator. Coding device for stabilizing protein EDA linked to EcnA/EcnB through a GSG linker.
false
false
_2012_
4206
18839
9
false
Use in conjunction with strain-expressing T7 polymerase.
false
Jonlin Chen
annotation2469853
1
BamHI cutsite
range2469853
1
390
395
annotation2469857
1
EcnA
range2469857
1
87
206
annotation2469856
1
TEV cutsite
range2469856
1
207
227
annotation2469860
1
T7
range2469860
1
1
21
annotation2469858
1
NdeI cutsite
range2469858
1
81
86
annotation2469852
1
Terminator
range2469852
1
396
438
annotation2469855
1
EcnB
range2469855
1
228
371
annotation2469859
1
RBS
range2469859
1
22
80
annotation2469854
1
6xHist tag
range2469854
1
372
389
BBa_K1595031_sequence
1
gtaatacgactcactatagggaatacaagctacttgttctttttgcaggtacctactagagaaagacaggaccctactagcatatgatgaaacgcctgattgtgctggtgctgctggcgagcaccctgctgaccggctgcaacaccgcgcgcggctttggcgaagatattaaacatctgggcaacagcattagccgcgcggcgagcgaaaacctgtatttccagggtatggtgaaaaaaaccattgcggcgatttttagcgtgctggtgctgagcaccgtgctgaccgcgtgcaacaccacccgcggcgtgggcgaagatattagcgatggcggcaacgcgattagcggcgcggcgaccaaagcgcagcagcatcatcatcatcatcatggatcctaaaaaaaaaaaccccgcccctgacagggcggggttttttttt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z