BBa_K1595031 1 BBa_K1595031 EcnA and EcnB from Escherichia coli str. K-12 substr. DH10B 2015-09-17T11:00:00Z 2016-02-03T10:54:52Z Synthesized by DNA 2.0 T7 + RBS + EDA + GSG + NdeI + EcnA + TEV + EcnB + 6xHist tag + BamHI + Terminator. Coding device for stabilizing protein EDA linked to EcnA/EcnB through a GSG linker. false false _2012_ 4206 18839 9 false Use in conjunction with strain-expressing T7 polymerase. false Jonlin Chen annotation2469853 1 BamHI cutsite range2469853 1 390 395 annotation2469857 1 EcnA range2469857 1 87 206 annotation2469856 1 TEV cutsite range2469856 1 207 227 annotation2469860 1 T7 range2469860 1 1 21 annotation2469858 1 NdeI cutsite range2469858 1 81 86 annotation2469852 1 Terminator range2469852 1 396 438 annotation2469855 1 EcnB range2469855 1 228 371 annotation2469859 1 RBS range2469859 1 22 80 annotation2469854 1 6xHist tag range2469854 1 372 389 BBa_K1595031_sequence 1 gtaatacgactcactatagggaatacaagctacttgttctttttgcaggtacctactagagaaagacaggaccctactagcatatgatgaaacgcctgattgtgctggtgctgctggcgagcaccctgctgaccggctgcaacaccgcgcgcggctttggcgaagatattaaacatctgggcaacagcattagccgcgcggcgagcgaaaacctgtatttccagggtatggtgaaaaaaaccattgcggcgatttttagcgtgctggtgctgagcaccgtgctgaccgcgtgcaacaccacccgcggcgtgggcgaagatattagcgatggcggcaacgcgattagcggcgcggcgaccaaagcgcagcagcatcatcatcatcatcatggatcctaaaaaaaaaaaccccgcccctgacagggcggggttttttttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z