BBa_K1597000
1
BBa_K1597000
Salt inducible promoter for B. subtilis
2015-09-08T11:00:00Z
2015-09-13T02:51:51Z
Bacillus subtilius genomic DNA
Bacillus subtilis is capable to cope with fluctuating salt concentrations. One of the genes involved is the proH, this is an 1-pyrroline-5-caboxylate reductase. A studie has shown that this gene is under control of the proH-promoter wich can be activated by a range of salt concentrations.
We have cloned this promoter out of the genome of bacillus and combined it with TasA (an amyloid producing gene) and GFP. The latter was used to validate the promoter.
false
false
_2014_
24529
24529
9
No part sequence
false
During the design primers were designed to add a prefix and suffix to the promoter.
false
Marieke Mulder
BBa_K1597000_sequence
1
gtctgaatttaaagagattggttttaaaccaaatcatacagaagtataccggtctttgcatgagcttcttgatgacgggatactaaaacaaattaaagtaaaaaaagaaggggctaagctccaggaagtcgtcctctatcaatttaaagattacgaagctgccaagctatataaaaaacagctgaaggtagagctgagctggatcgctgtaaaaaactgattgaaaaagctctctcagataatttttaatagaaacaaacacccggcgcagcagtgtgaaccgggtgttttctgcttttcattatacatatttgtcgaaaagaaacaatacaaatcaataatggccttcaaacttgacatcatttcctcacgtggtaacattttaaacgtgtgaaaatgcgaacaaaaaagcgaacaaaggagatgttaccgatctttgatcaaaagaaagtagcctttataggagcaggatc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z