LOCUS BBa_K1597001 5147 bp DNA linear 22-Dec-2024 DEFINITION ThrC integration backbone for B. subtilis ACCESSION BBa_K1597001 VERSION BBa_K1597001.1 FEATURES Location/Qualifiers misc_feature 1162..1964 /label=ThrC misc_feature 4361..5110 /label=ThrC CDS 334..1105 /label=erythromycin resistance gene CDS 3290..4150 /label=bla ORIGIN 1 tactagtagc ggccgctgca gtcgataacc ctaaagttat ggaaataaga cttagaagca 61 aacttaagag tgtgttgata gtgcattatc ttaaaatttt gtataatagg aattgaagtt 121 aaattagatg ctaaaaattt gtaattaaga aggagggatt cgtcatgttg gtattccaaa 181 tgcgtaatgt agataaaaca tctactgttt tgaaacagac taaaaacagt gattacgcag 241 ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta aacagataac 301 taaaattaca aacaaatcgt ttaacttctg tatttattta tagatgtatc acttcaggag 361 tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgaatg aaaaagtact 421 caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg aaattggaac 481 aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa cgtctattga 541 attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca ttcgtgtcac 601 tttaattcac caagatattc tacagtttca attccctaac aaacagaggt ataaaattgt 661 tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt ttgaaagcca 721 tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct tggatattca 781 ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc ttaagctgcc 841 agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac ttacccgcca 901 taccacagat gttccagata aatattggaa gctatatacg tactttgttt caaaatgggt 961 caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa tgaaacacgc 1021 caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt ttaatagtta 1081 tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt ggaaagttac 1141 acgttactaa agggaatgta gataaattat taggtatact actgacagct tccaaggagc 1201 taaagaggtc cctagcgcct acggggaatt tgtatcgaag tgtgcgaaca gctgggggaa 1261 gcgcctgatg ttttagcgat cccggtcgga aatgctggaa acatcactgc gtactggaag 1321 ggcttcaagg aatatcatga gaaaaacggc acaggccttc cgaaaatgcg cggctttgaa 1381 gctgagggtg cggcggcaat cgtgcgcaat gaagtgattg aaaatccgga aacaatagcg 1441 acagccattc gtatcggaaa cccggcaagc tgggacaaag ctgtaaaggc agccgaggaa 1501 tccaatggga aaattgacga agtcactgat gatgaaatcc ttcacgcata tcagctgatc 1561 gcccgtgtag aaggcgtgtt tgcagaacca ggttcttgcg cgtctatcgc aggagtgctg 1621 aaacaggtga aatccggaga aattccgaaa ggcagcaagg tcgtagctgt gttaacagga 1681 aacggactga aagatccgaa cacagcggtc gacatttcag aaatcaagcc tgtcacattg 1741 ccgactgatg aagacagcat ccttgaatat gtaaaaggag cggcccgtgt atgaacgaag 1801 ccgacatgct gttctctgtc actgttcccg gaagcacagc taacctaggc cccggctttg 1861 attcagtcgg aatggcgctc agcagatatt tgaagctgac cgtctttgaa agcgacaaat 1921 ggtcttttga ggctgaaaca gaaacagtcg ccggaattaa ttcgagctcc tttaacttcc 1981 tcaccagtag ttgtatcggt accataagta gaagcagcaa cccaagtagc tttaccagca 2041 tccggttcaa ccagcatagt aagaatctta ctggacatcg gcagttcttc gaacagtgcg 2101 ccaactacca gctctttctc cagaatgggc tatacctctt ttacctaaag agcatgtaac 2161 tttactggat atagctagaa aaggctatcg gggagagtgt gatgataagt gggaaggact 2221 atattcaaag gtgaaagcac tcgttaagta tatgaaaaat tctatagaaa cttctctcaa 2281 ttaggctaat tttattgcaa taacaggtgc ttacttttct ggagttcttt agcaaatttt 2341 tttattagct gaacttagta ttagtggcca tactcctcca atccaaagct atttagaaag 2401 attactatat cctcaaacag gcggtaaccg gcctcttcat cgggaatgcg cgcgaccttc 2461 agcatcgccg gcatgtcccc ctggcggacg ggaagtatcc agctcgaggt cgggccgcgt 2521 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 2581 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 2641 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 2701 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 2761 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 2821 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 2881 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 2941 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 3001 agccagttac cttcggaaaa agagttgata gctcttgatc cggcaaacaa accaccgctg 3061 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 3121 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 3181 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 3241 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 3301 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 3361 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 3421 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 3481 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 3541 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 3601 ttgctgccgg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 3661 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 3721 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 3781 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 3841 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 3901 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 3961 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 4021 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 4081 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 4141 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 4201 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 4261 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata 4321 aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt aattcatgta aaagatgagg 4381 ttggttcatt ctcgaaaatt acatctgtgt tctcagagcg gggcgtgacg tttgaaaaaa 4441 tccttcagct gccaattaaa ggccatgatg agttagctga aatcgtaatt gtcacacatc 4501 atacatcaga agctgatttc agtgatatcc tgcaaaacct aaatgatttg gaagtcgttc 4561 aagaagtcaa aagcacatat cgtgtagaag ggaacggttg gagctaatgt ggaaaggact 4621 tatccatcaa tataaagaat ttttacctgt aacagatcaa acaccggcgc taactttaca 4681 tgaaggaaac acacctctta ttcacctgcc gaagctgtct gagcagctcg gaattgagct 4741 tcatgtcaaa acggaaggcg tcaatcctac gggatcattt aaagatcgcg gaatggttat 4801 ggctgtggca aaggcaaaag aagaaggcaa tgacacgatt atgtgcgcgt caacaggtaa 4861 cacttccgct gctgcggcag catatgcagc ccgtgctaac atgaaatgca ttgtcatcat 4921 cccgaacgga aaaattgcat ttggaaaact cgctcaagct gtcatgtacg gagccgagat 4981 tatcgcaatt gacggaaact ttgacgatgc gcttaaaatt gtccgttcca tctgtgagaa 5041 atcaccgatt gcccttgtca actcagtcaa cccttaccgc attgaaggcc aaaaaactgc 5101 tgccttcgga tcctagaagc ttatcgaatt cgcggccgct tctagag //