LOCUS BBa_K1598002 2982 bp DNA linear 24-Nov-2024 DEFINITION Lac induced expression cassette + TPH1 (tryptophan hydroxylase 1) ACCESSION BBa_K1598002 VERSION BBa_K1598002.1 FEATURES Location/Qualifiers misc_feature 18..324 /label=f1 origin misc_feature 1..455 /label=BBa_K314110 misc_feature 464..1555 /label=BBa_K314111 misc_feature 1566..1582 /label=lac O1 promoter 1583..1588 /label=-35 misc_feature 1589..1605 /label=lac O1 promoter 1606..1611 /label=-10 misc_feature 1564..1617 /label=BBa_R0011 promoter 1564..1617 /label=BBa_R0011 misc_feature 1631..1634 /label=conserved RBS 1627..1638 /label=BBa_B0034 misc_feature 1..1638 /label=BBa_K314103 CDS 1645..2982 /label=BBa_K1598001 ORIGIN 1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 421 acgcgaattt taacaaaata ttaacgttta caatttacta gaggtggtga atgtgaaacc 481 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 541 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 601 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 661 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 721 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 781 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 841 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 901 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 961 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 1021 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 1081 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 1141 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 1201 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 1261 cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 1321 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 1381 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 1441 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 1501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgatacta 1561 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata 1621 ctagagaaag aggagaaata ctagatgatt gaggacaaca aagagaataa agatcattcc 1681 ctggagcgcg gtcgcgcgtc cctgatcttc tcattgaaaa atgaagtcgg aggcctgata 1741 aaggcattga aaatctttca ggaaaaacat gtaaacctgt tgcacattga atcacgcaag 1801 agcaaacgtc ggaactcaga gtttgaaatc tttgtcgatt gtgatattaa tcgggaacag 1861 ctgaatgaca tttttcatct gttgaagtct cacaccaatg tgctctctgt aaatctgcca 1921 gataatttca cgctcaaaga agatggtatg gaaacggtcc catggtttcc caaaaagatt 1981 tcggacctgg accattgtgc aaaccgcgtg ctgatgtacg gttcagaact tgacgccgat 2041 cacccagggt tcaaggacaa cgtttatcgg aaacgccgga aatactttgc cgacctagcc 2101 atgaattaca aacatggcga cccaatcccg aaagtagagt tcacggaaga agaaatcaag 2161 acgtggggaa ccgtatttca ggaacttaac aagctgtacc cgacgcacgc gtgtcgcgaa 2221 tatctgaaaa acttgccgtt gctctctaaa tattgcggtt accgcgaaga taatatccca 2281 caactggaag atgtgagcaa cttcctgaaa gaacgtaccg gcttctcaat tcgcccggtg 2341 gccggttatc tgtcaccgcg tgacttcctc tctggcttag cttttcgcgt gttccattgt 2401 actcagtatg tgcgtcacag ctcggacccc ttttataccc ctgagcccga cacctgtcac 2461 gaattgctgg gtcatgtccc tttgctggcg gaaccgtcct ttgcgcagtt tagccaagaa 2521 atcggtttag ccagcctggg tgcttccgaa gaagcggtcc aaaaactggc gacatgctat 2581 ttttttacgg tcgagtttgg cctttgcaaa caggacggtc agcttcgggt ttttggcgcg 2641 gggctgttga gttcaatctc agaacttaag cacgccctgt caggtcatgc gaaagtgaaa 2701 ccttttgacc ctaaaatcac ctgtaagcag gaatgtctaa tcaccacgtt tcaggacgtc 2761 tattttgtga gtgaatcctt tgaagatgct aaagaaaaaa tgcgtgagtt taccaaaaca 2821 atcaaacgcc cgttcggtgt aaaatataac ccttacacgc gctcaatcca gattttaaaa 2881 gacactaagt ccattacttc ggccatgaat gagttacaac acgatttgga cgtggtgagt 2941 gatgccctgg cgaaagtgtc gcgtaaaccg agtatttaat aa //