BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K314111 1 BBa_K314111 Lac I 2010-10-07T11:00:00Z 2015-05-08T01:11:55Z Pet 29 vector Lac I gene with promoter and RBS false false _496_ 0 6395 9 It's complicated false false IGEM 2010 Team Washington BBa_K314110 1 f1 ori f1 origin 2010-10-07T11:00:00Z 2015-05-08T01:11:55Z Pet 29 vector Released HQ 2013 Phage origin recognized by M13. Used to make SS DNA when infected with M13k07 helper phage false false _496_ 0 6395 9 In stock true false IGEM 2010 Team Washington annotation2115768 1 f1 origin range2115768 1 18 324 BBa_K314103 1 BBa_K314103 Lac induced expression cassette 2010-10-07T11:00:00Z 2015-05-08T01:11:55Z construct Released HQ 2013 Lac induced protein expression insert includes f1 origin, Lac I gene and RBS false false _496_ 0 6395 9 In stock true false Tomas Huber, Justin Siegel component2091083 1 BBa_R0011 component2091082 1 BBa_K314111 component2091081 1 BBa_K314110 component2091089 1 BBa_B0034 annotation2091081 1 BBa_K314110 range2091081 1 1 455 annotation2091089 1 BBa_B0034 range2091089 1 1627 1638 annotation2091083 1 BBa_R0011 range2091083 1 1564 1617 annotation2091082 1 BBa_K314111 range2091082 1 464 1555 BBa_K1598001 1 BBa_K1598001 TPH1 (Tryptophan hydroxylase 1) 2015-06-17T11:00:00Z 2015-08-27T02:25:18Z Human (Homo sapiens) Converts the amino acid tryptophan into 5-HTP, which is a precursor to serotonin. Soon to be edit false false _2015_ 27180 27256 9 false Optimised for E.coli, removed illegal restriction enzyme sites false Marta Napiorkowska BBa_K1598002 1 BBa_K1598002 Lac induced expression cassette + TPH1 (tryptophan hydroxylase 1) 2015-07-21T11:00:00Z 2015-08-27T02:06:06Z Lac induced expression cassette from 2014 iGEM distribution Tryptophan hydroxylase 1 - TPH1 human gene, synthesized To be edited false false _2015_ 27180 27180 9 true Codon optimized for E.coli false Marta Napiorkowska component2433301 1 BBa_K314103 component2433302 1 BBa_K1598001 annotation2433302 1 BBa_K1598001 range2433302 1 1645 2982 annotation2433301 1 BBa_K314103 range2433301 1 1 1638 BBa_R0011 1 lacI+pL Promoter (lacI regulated, lambda pL hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). false true _1_ 0 24 7 In stock false <P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs. true Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton annotation2002 1 -10 range2002 1 43 48 annotation1999 1 lac O1 range1999 1 3 19 annotation2000 1 -35 range2000 1 20 25 annotation7064 1 BBa_R0011 range7064 1 1 54 annotation2001 1 lac O1 range2001 1 26 42 BBa_K314110_sequence 1 acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatt BBa_B0034_sequence 1 aaagaggagaaa BBa_K1598001_sequence 1 atgattgaggacaacaaagagaataaagatcattccctggagcgcggtcgcgcgtccctgatcttctcattgaaaaatgaagtcggaggcctgataaaggcattgaaaatctttcaggaaaaacatgtaaacctgttgcacattgaatcacgcaagagcaaacgtcggaactcagagtttgaaatctttgtcgattgtgatattaatcgggaacagctgaatgacatttttcatctgttgaagtctcacaccaatgtgctctctgtaaatctgccagataatttcacgctcaaagaagatggtatggaaacggtcccatggtttcccaaaaagatttcggacctggaccattgtgcaaaccgcgtgctgatgtacggttcagaacttgacgccgatcacccagggttcaaggacaacgtttatcggaaacgccggaaatactttgccgacctagccatgaattacaaacatggcgacccaatcccgaaagtagagttcacggaagaagaaatcaagacgtggggaaccgtatttcaggaacttaacaagctgtacccgacgcacgcgtgtcgcgaatatctgaaaaacttgccgttgctctctaaatattgcggttaccgcgaagataatatcccacaactggaagatgtgagcaacttcctgaaagaacgtaccggcttctcaattcgcccggtggccggttatctgtcaccgcgtgacttcctctctggcttagcttttcgcgtgttccattgtactcagtatgtgcgtcacagctcggaccccttttatacccctgagcccgacacctgtcacgaattgctgggtcatgtccctttgctggcggaaccgtcctttgcgcagtttagccaagaaatcggtttagccagcctgggtgcttccgaagaagcggtccaaaaactggcgacatgctatttttttacggtcgagtttggcctttgcaaacaggacggtcagcttcgggtttttggcgcggggctgttgagttcaatctcagaacttaagcacgccctgtcaggtcatgcgaaagtgaaaccttttgaccctaaaatcacctgtaagcaggaatgtctaatcaccacgtttcaggacgtctattttgtgagtgaatcctttgaagatgctaaagaaaaaatgcgtgagtttaccaaaacaatcaaacgcccgttcggtgtaaaatataacccttacacgcgctcaatccagattttaaaagacactaagtccattacttcggccatgaatgagttacaacacgatttggacgtggtgagtgatgccctggcgaaagtgtcgcgtaaaccgagtatttaataa BBa_K314111_sequence 1 gtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtga BBa_K1598002_sequence 1 acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagaggtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgattgaggacaacaaagagaataaagatcattccctggagcgcggtcgcgcgtccctgatcttctcattgaaaaatgaagtcggaggcctgataaaggcattgaaaatctttcaggaaaaacatgtaaacctgttgcacattgaatcacgcaagagcaaacgtcggaactcagagtttgaaatctttgtcgattgtgatattaatcgggaacagctgaatgacatttttcatctgttgaagtctcacaccaatgtgctctctgtaaatctgccagataatttcacgctcaaagaagatggtatggaaacggtcccatggtttcccaaaaagatttcggacctggaccattgtgcaaaccgcgtgctgatgtacggttcagaacttgacgccgatcacccagggttcaaggacaacgtttatcggaaacgccggaaatactttgccgacctagccatgaattacaaacatggcgacccaatcccgaaagtagagttcacggaagaagaaatcaagacgtggggaaccgtatttcaggaacttaacaagctgtacccgacgcacgcgtgtcgcgaatatctgaaaaacttgccgttgctctctaaatattgcggttaccgcgaagataatatcccacaactggaagatgtgagcaacttcctgaaagaacgtaccggcttctcaattcgcccggtggccggttatctgtcaccgcgtgacttcctctctggcttagcttttcgcgtgttccattgtactcagtatgtgcgtcacagctcggaccccttttatacccctgagcccgacacctgtcacgaattgctgggtcatgtccctttgctggcggaaccgtcctttgcgcagtttagccaagaaatcggtttagccagcctgggtgcttccgaagaagcggtccaaaaactggcgacatgctatttttttacggtcgagtttggcctttgcaaacaggacggtcagcttcgggtttttggcgcggggctgttgagttcaatctcagaacttaagcacgccctgtcaggtcatgcgaaagtgaaaccttttgaccctaaaatcacctgtaagcaggaatgtctaatcaccacgtttcaggacgtctattttgtgagtgaatcctttgaagatgctaaagaaaaaatgcgtgagtttaccaaaacaatcaaacgcccgttcggtgtaaaatataacccttacacgcgctcaatccagattttaaaagacactaagtccattacttcggccatgaatgagttacaacacgatttggacgtggtgagtgatgccctggcgaaagtgtcgcgtaaaccgagtatttaataa BBa_R0011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca BBa_K314103_sequence 1 acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagaggtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z