LOCUS       BBa_K1600001             731 bp    DNA     linear       08-Dec-2025
DEFINITION  IPTG Promoter+RBS+EIIA+double terminator
ACCESSION   BBa_K1600001
VERSION     BBa_K1600001.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    1..75
                     /label=BBa_K215000
     misc_feature    82..84
                     /label=ATG start codon
     CDS             82..594
                     /label=IIA(Glc) coding region
     misc_feature    589..594
                     /label=TAA TAA stop codon
     CDS             82..594
                     /label=BBa_K1508002
     stem_loop       614..657
                     /label=stem_loop
     misc_feature    603..682
                     /label=BBa_B0010
     terminator      603..682
                     /label=BBa_B0010
     stem_loop       698..717
                     /label=T7 TE
     misc_feature    724..724
                     /label=stop
     polyA_site      718..731
                     /label=polya
     misc_feature    691..731
                     /label=BBa_B0012
     terminator      691..731
                     /label=BBa_B0012
     terminator      603..731
                     /label=BBa_B0015
     misc_feature    82..731
                     /label=BBa_K1508003
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgggtttg ttcgataaac tgaaatctct ggtttccgac
      121 gacaagaagg ataccggaac tattgagatc attgctccgc tctctggcga gatcgtcaat
      181 atcgaagacg tgccggatgt cgtttttgcg gaaaaaatcg ttggtgatgg tattgctatc
      241 aaaccaacgg gtaacaaaat ggtcgcgcca gtagacggca ccattggtaa aatctttgaa
      301 accaaccacg cattctctat cgaatctgat agcggcgttg aactgttcgt ccacttcggt
      361 atcgacaccg ttgaactgaa aggcgaaggc ttcaagcgta ttgctgaaga aggtcagcgc
      421 gtgaaagttg gcgatactgt cattgaattt gatctgccgc tgctggaaga gaaagccaag
      481 tctaccctga ctccggttgt tatctccaac atggacgaaa tcaaagaact gatcaaactg
      541 tccggtagcg taaccgtggg tgaaaccccg gttatccgca tcaagaagta ataatactag
      601 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      661 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
      721 ctgcgtttat a
//