BBa_K1600001
type: ComponentDefinition
persistent identity: BBa K1600001
display id: BBa_K1600001
version: 1
was generated by: iGEM to SBOL conversion
description: IPTG Promoter+RBS+EIIA+double terminator
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T07:42:20Z
mutable provenance: E. Coli K12 substrain
owned by: james
owned by: myers
mutable description: This composite part was designed to upregulate intake of glucose by the cell. Upon further characterization, the preliminary data showed no upregulation of glucose intake. This may be due to the fact that EIIA alone cannot uptake the glucose as it itself is not the membrane protein of the glucose-related sections of the phosphotransferase system (PTS). We hypothesize that upregulating EIIBC (BBa_K1600000), which is a membrane protein complex in addition to EIIA will result in increased glucose uptake.
discontinued: false
dominant: false
experience: experience/None
group u list: _2017_
m user id: 24520
owner id: 24520
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: None.
star: false
creator: Rena Wang Yuan
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1508003
component: BBa_K215000
sequence annotation: BBa_K215000
sequence annotation: BBa_K1508003
sequence: https://synbiohub.org/public/igem/BBa_K1600001_sequence/1

BBa_K1508003
type: Component
persistent identity: component2472379
display id: component2472379
version: 1
top level: BBa_K1600001
definition: BBa_K1508003
access: public

BBa_K215000
type: Component
persistent identity: component2472367
display id: component2472367
version: 1
top level: BBa_K1600001
definition: BBa_K215000
access: public

BBa_K215000
type: SequenceAnnotation
persistent identity: annotation2472367
display id: annotation2472367
version: 1
top level: BBa_K1600001
location: https://synbiohub.org/public/igem/BBa_K1600001/annotation2472367/range2472367/1
component: BBa_K215000

https://synbiohub.org/public/igem/BBa_K1600001/annotation2472367/range2472367/1
type: Range
persistent identity: range2472367
display id: range2472367
version: 1
top level: BBa_K1600001
direction: direction/reverse
start: 1
end: 75
orientation: inline

BBa_K1508003
type: SequenceAnnotation
persistent identity: annotation2472379
display id: annotation2472379
version: 1
top level: BBa_K1600001
location: https://synbiohub.org/public/igem/BBa_K1600001/annotation2472379/range2472379/1
component: BBa_K1508003

https://synbiohub.org/public/igem/BBa_K1600001/annotation2472379/range2472379/1
type: Range
persistent identity: range2472379
display id: range2472379
version: 1
top level: BBa_K1600001
direction: direction/reverse
start: 82
end: 731
orientation: inline

BBa_K215000
type: ComponentDefinition
persistent identity: BBa K215000
display id: BBa_K215000
version: 1
was generated by: iGEM to SBOL conversion
description: R0011+B0034, strong IPTG-inducible promoter with strong RBS.
created: 2009-09-29T11:00:00Z
modified: 2015-05-08T01:11:29Z
mutable provenance: existing registry parts
owned by: james
owned by: myers
mutable description: This part contains the strong, IPTG-inducible promoter R0011, combined with the strong RBS B0034. This part can be used as a strong protein expression system when combined with a protein coding sequence.
discontinued: false
dominant: false
experience: experience/Works
group u list: _320_
m user id: 0
owner id: 2811
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: This part should offer greater levels of expression when compared to similar parts using the natural lac promoter (R0010).
star: false
creator: Chris Eiben
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_R0011
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K215000_sequence/1

BBa_B0034
type: Component
persistent identity: component2027441
display id: component2027441
version: 1
top level: BBa_K215000
definition: BBa_B0034
access: public

BBa_R0011
type: Component
persistent identity: component2027435
display id: component2027435
version: 1
top level: BBa_K215000
definition: lacI+pL
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2027435
display id: annotation2027435
version: 1
top level: BBa_K215000
location: https://synbiohub.org/public/igem/BBa_K215000/annotation2027435/range2027435/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K215000/annotation2027435/range2027435/1
type: Range
persistent identity: range2027435
display id: range2027435
version: 1
top level: BBa_K215000
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2027441
display id: annotation2027441
version: 1
top level: BBa_K215000
location: https://synbiohub.org/public/igem/BBa_K215000/annotation2027441/range2027441/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K215000/annotation2027441/range2027441/1
type: Range
persistent identity: range2027441
display id: range2027441
version: 1
top level: BBa_K215000
direction: direction/reverse
start: 64
end: 75
orientation: inline

BBa_K1508003
type: ComponentDefinition
persistent identity: BBa K1508003
display id: BBa_K1508003
version: 1
was generated by: iGEM to SBOL conversion
description: IIA(Glc) + double terminator
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:47Z
mutable provenance: IIA(Glc) gene from Escherichia coli; double terminator from iGEM Registry
owned by: james
owned by: myers
mutable description: E. coli IIA(Glc) gene (BBa_K1508002) followed by double terminator (BBa_B0015)
discontinued: false
dominant: false
experience: experience/None
group u list: _1888_
m user id: 0
owner id: 16581
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Construct was assembled by suffix insertion, namely BBa_B0015 fragment was inserted into BBa_K1508002 backbone.
star: false
creator: Fahmi Dwilaksono, Indah Nurulita, Rian Adha Ardinata, Mochammad Isro Alfajri, Arief Budi Witarto
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_K1508002
sequence annotation: BBa_K1508002
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1508003_sequence/1

BBa_B0015
type: Component
persistent identity: component2407582
display id: component2407582
version: 1
top level: BBa_K1508003
definition: BBa_B0015
access: public

BBa_K1508002
type: Component
persistent identity: component2407575
display id: component2407575
version: 1
top level: BBa_K1508003
definition: BBa_K1508002
access: public

BBa_K1508002
type: SequenceAnnotation
persistent identity: annotation2407575
display id: annotation2407575
version: 1
top level: BBa_K1508003
location: https://synbiohub.org/public/igem/BBa_K1508003/annotation2407575/range2407575/1
component: BBa_K1508002

https://synbiohub.org/public/igem/BBa_K1508003/annotation2407575/range2407575/1
type: Range
persistent identity: range2407575
display id: range2407575
version: 1
top level: BBa_K1508003
direction: direction/reverse
start: 1
end: 513
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2407582
display id: annotation2407582
version: 1
top level: BBa_K1508003
location: https://synbiohub.org/public/igem/BBa_K1508003/annotation2407582/range2407582/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1508003/annotation2407582/range2407582/1
type: Range
persistent identity: range2407582
display id: range2407582
version: 1
top level: BBa_K1508003
direction: direction/reverse
start: 522
end: 650
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_K1508002
type: ComponentDefinition
persistent identity: BBa K1508002
display id: BBa_K1508002
version: 1
was generated by: iGEM to SBOL conversion
description: IIA(Glc) - E.coli glucose-specific phosphotransferase enzyme IIA
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:47Z
mutable provenance: Escherichia coli DH5alpha genome
owned by: james
owned by: myers
mutable description: Phosphotransferase system (PTS) is a major carbohydrate active -transport system, which is executed by a cascade of protein rally. For glucose uptake, IIA(Glc) is the only enzyme specific to glucose transport. We measure the concentration of glucose in the medium using catabolite repression mechanism toward lacI promoter activity upstream of a reporter gene, mRFP. We expect by over-expressing IIA(Glc) in E. coli, we will obtain different range of glucose standard curve in our measurement system.
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1888_
m user id: 0
owner id: 16581
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The gene coding for IIA(Glc) from E.coli - crr - was isolated by colony PCR using forward primer which contains with restriction sites of EcoRI and XbaI and reverse primers which contains restriction site for SpeI and a double stop codon.
star: false
creator: Fahmi Dwilaksono, Indah Nurulita, Rian Adha Ardinata, Mochammad Isro Alfajri, Arief Budi Witarto
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: ATG start codon
sequence annotation: IIA(Glc) coding region
sequence annotation: TAA TAA stop codon
sequence: https://synbiohub.org/public/igem/BBa_K1508002_sequence/1

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2407415
display id: annotation2407415
version: 1
top level: BBa_K1508002
location: https://synbiohub.org/public/igem/BBa_K1508002/annotation2407415/range2407415/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1508002/annotation2407415/range2407415/1
type: Range
persistent identity: range2407415
display id: range2407415
version: 1
top level: BBa_K1508002
direction: direction/forward
start: 1
end: 3
orientation: inline

IIA(Glc) coding region
type: SequenceAnnotation
persistent identity: annotation2407417
display id: annotation2407417
version: 1
top level: BBa_K1508002
location: https://synbiohub.org/public/igem/BBa_K1508002/annotation2407417/range2407417/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1508002/annotation2407417/range2407417/1
type: Range
persistent identity: range2407417
display id: range2407417
version: 1
top level: BBa_K1508002
direction: direction/forward
start: 1
end: 513
orientation: inline

TAA TAA stop codon
type: SequenceAnnotation
persistent identity: annotation2407420
display id: annotation2407420
version: 1
top level: BBa_K1508002
location: https://synbiohub.org/public/igem/BBa_K1508002/annotation2407420/range2407420/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1508002/annotation2407420/range2407420/1
type: Range
persistent identity: range2407420
display id: range2407420
version: 1
top level: BBa_K1508002
direction: direction/forward
start: 508
end: 513
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1600001_sequence/1
type: Sequence
persistent identity: BBa K1600001 sequence
display id: BBa_K1600001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgggtttgttcgataaactgaaatctctggtttccgacgacaagaaggataccggaactattgagatcattgctccgctctctggcgagatcgtcaatatcgaagacgtgccggatgtcgtttttgcggaaaaaatcgttggtgatggtattgctatcaaaccaacgggtaacaaaatggtcgcgccagtagacggcaccattggtaaaatctttgaaaccaaccacgcattctctatcgaatctgatagcggcgttgaactgttcgtccacttcggtatcgacaccgttgaactgaaaggcgaaggcttcaagcgtattgctgaagaaggtcagcgcgtgaaagttggcgatactgtcattgaatttgatctgccgctgctggaagagaaagccaagtctaccctgactccggttgttatctccaacatggacgaaatcaaagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K215000_sequence/1
type: Sequence
persistent identity: BBa K215000 sequence
display id: BBa_K215000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1508003_sequence/1
type: Sequence
persistent identity: BBa K1508003 sequence
display id: BBa_K1508003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgggtttgttcgataaactgaaatctctggtttccgacgacaagaaggataccggaactattgagatcattgctccgctctctggcgagatcgtcaatatcgaagacgtgccggatgtcgtttttgcggaaaaaatcgttggtgatggtattgctatcaaaccaacgggtaacaaaatggtcgcgccagtagacggcaccattggtaaaatctttgaaaccaaccacgcattctctatcgaatctgatagcggcgttgaactgttcgtccacttcggtatcgacaccgttgaactgaaaggcgaaggcttcaagcgtattgctgaagaaggtcagcgcgtgaaagttggcgatactgtcattgaatttgatctgccgctgctggaagagaaagccaagtctaccctgactccggttgttatctccaacatggacgaaatcaaagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1508002_sequence/1
type: Sequence
persistent identity: BBa K1508002 sequence
display id: BBa_K1508002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgggtttgttcgataaactgaaatctctggtttccgacgacaagaaggataccggaactattgagatcattgctccgctctctggcgagatcgtcaatatcgaagacgtgccggatgtcgtttttgcggaaaaaatcgttggtgatggtattgctatcaaaccaacgggtaacaaaatggtcgcgccagtagacggcaccattggtaaaatctttgaaaccaaccacgcattctctatcgaatctgatagcggcgttgaactgttcgtccacttcggtatcgacaccgttgaactgaaaggcgaaggcttcaagcgtattgctgaagaaggtcagcgcgtgaaagttggcgatactgtcattgaatttgatctgccgctgctggaagagaaagccaagtctaccctgactccggttgttatctccaacatggacgaaatcaaagaactgatcaaactgtccggtagcgtaaccgtgggtgaaaccccggttatccgcatcaagaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z