##gff-version 3
##sequence-region BBa_K1600002 1 2942
BBa_K1600002	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_K1600002	.	terminator	2814	2942	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1600002	.	terminator	2902	2942	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1600002	.	engineered_region	2902	2942	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1600002	.	stem_loop	2909	2928	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1600002	.	stop_codon	2935	2935	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1600002	.	polyA_site	2929	2942	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1600002	.	terminator	2814	2893	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1600002	.	engineered_region	2814	2893	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1600002	.	stem_loop	2825	2868	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1600002	.	engineered_region	1369	2805	.	+	0	ID=BBa_K322921;Name=BBa_K322921
BBa_K1600002	.	ribosome_entry_site	1369	1371	.	+	0	ID=annotation2098459;Name=RBS;Parent=BBa_K322921
BBa_K1600002	.	CDS	1381	2802	.	+	0	ID=annotation2098480;Name=Coding sequence;Parent=BBa_K322921
BBa_K1600002	.	point_mutation	1579	1584	.	+	0	ID=annotation2098481;Name=Removed EcoRI site;Parent=BBa_K322921
BBa_K1600002	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1600002	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1600002	.	CDS	62	1360	.	+	0	ID=BBa_K118016;Name=BBa_K118016
BBa_K1600002	.	point_mutation	1129	1134	.	+	0	ID=annotation1978559;Name=EcoRI site removed;Parent=BBa_K118016
BBa_K1600002	.	sequence_alteration	1067	1069	.	+	0	ID=annotation1978557;Name=G336D;Parent=BBa_K118016
BBa_K1600002	.	point_mutation	632	637	.	+	0	ID=annotation1978558;Name=EcoRI site removed;Parent=BBa_K118016
>BBa_K1600002
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggttagtttagagaag
aacgatcacttaatgttggcgcgccagctgccattgaaatctgttgccctgatactggcgggaggacgtggtacccgcc
tgaaggatttaaccaataagcgagcaaaaccggccgtacacttcggcggtaagttccgcattatcgactttgcgctgtc
taactgcatcaactccgggatccgtcgtatgggcgtgatcacccagtaccagtcccacactctggtgcagcacattcag
cgcggctggtcattcttcaatgaagaaatgaacgagtttgtcgatctgctgccagcacagcagagaatgaaaggggaaa
actggtatcgcggcaccgcagatgcggtcacccaaaacctcgacattatccgccgttataaagcggaatacgtggtgat
cctggcgggcgaccatatctacaagcaagactactcgcgtatgcttatcgatcacgtcgaaaaaggcgcacgttgcacc
gttgcttgtatgccagtaccgattgaagaagcctccgcatttggcgttatggcggttgatgagaacgataaaattatcg
aatttgttgaaaaacctgctaacccgccgtcaatgccgaacgatccgagcaaatctctggcgagtatgggtatctacgt
ctttgacgccgactatctgtatgaactgctggaagaagacgatcgcgatgagaactccagccacgactttggcaaagat
ttgattcccaagatcaccgaagccggtctggcctatgcgcacccgttcccgctctcttgcgtacaatccgacccggatg
ccgagccgtactggcgcgatgtgggtacgctggaagcttactggaaagcgaacctcgatctggcctctgtggtgccgga
actggatatgtacgatcgcaattggccaattcgcacctacaatgaatcattaccgccagcgaaattcgtgcaggatcgc
tccggtagccacgggatgacccttaactcactggtttccgacggttgtgtgatctccggttcggtggtggtgcagtccg
ttctgttctcgcgcgttcgcgtgaactcattctgcaacattgattccgccgtattgttaccggaagtatgggtaggtcg
ctcgtgccgtctgcgccgctgcgtcatcgatcgtgcttgtgttattccggaaggcatggtgattggtgaaaacgcagag
gaagatgcacgtcgtttctatcgttcagaagaaggcatcgtgctggtaacgcgcgaaatgctacggaagttagggcata
aacaggagcgataataatactagaggagacatgaacgatgaacatcaaaaagtttgcaaaacaagcaacagtattaacc
tttactaccgcactgctggcaggaggcgcaactcaagcgtttgcgaaagaaacgaaccaaaagccatataaggaaacat
acggcatttcccatattacacgccatgatatgctgcaaatccctgaacagcaaaaaaatgaaaaatatcaagttcctga
gttcgattcgtccacaattaaaaatatctcttctgcaaaaggcctggacgtttgggacagctggccattacaaaacgct
gacggcactgtcgcaaactatcacggctaccacatcgtctttgcattagccggagatcctaaaaatgcggatgacacat
cgatttacatgttctatcaaaaagtcggcgaaacttctattgacagctggaaaaacgctggccgcgtctttaaagacag
cgacaaattcgatgcaaatgattctatcctaaaagaccaaacacaagaatggtcaggttcagccacatttacatctgac
ggaaaaatccgtttattctacactgatttctccggtaaacattacggcaaacaaacactgacaactgcacaagttaacg
tatcagcatcagacagctctttgaacatcaacggtgtagaggattataaatcaatctttgacggtgacggaaaaacgta
tcaaaatgtacagcagttcatcgatgaaggcaactacagctcaggcgacaaccatacgctgagagatcctcactacgta
gaagataaaggccacaaatacttagtatttgaagcaaacactggaactgaagatggctaccaaggcgaagaatctttat
ttaacaaagcatactatggcaaaagcacatcattcttccgtcaagaaagtcaaaaacttctgcaaagcgataaaaaacg
cacggctgagttagcaaacggcgctctcggtatgattgagctaaacgatgattacacactgaaaaaagtgatgaaaccg
ctgattgcatctaacacagtaacagatgaaattgaacgcgcgaacgtctttaaaatgaacggcaaatggtacctgttca
ctgactcccgcggatcaaaaatgacgattgacggcattacgtctaacgatatttacatgcttggttatgtttctaattc
tttaactggcccatacaagccgctgaacaaaactggccttgtgttaaaaatggatcttgatcctaacgatgtaaccttt
acttactcacacttcgctgtacctcaagcgaaaggaaacaatgtcgtgattacaagctatatgacaaacagaggattct
acgcagacaaacaatcaacgtttgcgccaagcttcctgctgaacatcaaaggcaagaaaacatctgttgtcaaagacag
catccttgaacaaggacaattaacagttaacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttata