BBa_K1600002
type: ComponentDefinition
persistent identity: BBa K1600002
display id: BBa_K1600002
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter+RBS+SacB+RBS+GlgC+Terminator
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T09:43:22Z
mutable provenance: This part is composed of BBa_J231000 (promoter), BBa_R0034 (RBS), BBa_K118016 (ADP glucose-phosphorylase), BBa_R0034 (RBS), BBa_XXXXXX (SacB), and BBa_B0015 (double terminator).
owned by: james
owned by: myers
mutable description: This part is composed of BBa_J231000 (promoter), BBa_R0034 (RBS), BBa_K118016 (ADP glucose-phosphorylase), BBa_R0034 (RBS), BBa_XXXXXX (SacB), and BBa_B0015 (double terminator).
discontinued: false
dominant: false
experience: experience/None
group u list: _2017_
m user id: 24520
owner id: 24519
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Supraja Shivakumar Chittari
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_K322921
component: BBa_K118016
component: BBa_B0015
component: BBa_B0034
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_B0034
sequence annotation: BBa_K118016
sequence annotation: BBa_K322921
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1600002_sequence/1

BBa_K322921
type: Component
persistent identity: component2473734
display id: component2473734
version: 1
top level: BBa_K1600002
definition: sacB
access: public

BBa_K118016
type: Component
persistent identity: component2473730
display id: component2473730
version: 1
top level: BBa_K1600002
definition: BBa_K118016
access: public

BBa_B0015
type: Component
persistent identity: component2473741
display id: component2473741
version: 1
top level: BBa_K1600002
definition: BBa_B0015
access: public

BBa_B0034
type: Component
persistent identity: component2473726
display id: component2473726
version: 1
top level: BBa_K1600002
definition: BBa_B0034
access: public

BBa_J23100
type: Component
persistent identity: component2473724
display id: component2473724
version: 1
top level: BBa_K1600002
definition: BBa_J23100
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2473724
display id: annotation2473724
version: 1
top level: BBa_K1600002
location: https://synbiohub.org/public/igem/BBa_K1600002/annotation2473724/range2473724/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1600002/annotation2473724/range2473724/1
type: Range
persistent identity: range2473724
display id: range2473724
version: 1
top level: BBa_K1600002
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2473726
display id: annotation2473726
version: 1
top level: BBa_K1600002
location: https://synbiohub.org/public/igem/BBa_K1600002/annotation2473726/range2473726/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1600002/annotation2473726/range2473726/1
type: Range
persistent identity: range2473726
display id: range2473726
version: 1
top level: BBa_K1600002
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K118016
type: SequenceAnnotation
persistent identity: annotation2473730
display id: annotation2473730
version: 1
top level: BBa_K1600002
location: https://synbiohub.org/public/igem/BBa_K1600002/annotation2473730/range2473730/1
component: BBa_K118016

https://synbiohub.org/public/igem/BBa_K1600002/annotation2473730/range2473730/1
type: Range
persistent identity: range2473730
display id: range2473730
version: 1
top level: BBa_K1600002
direction: direction/reverse
start: 62
end: 1360
orientation: inline

BBa_K322921
type: SequenceAnnotation
persistent identity: annotation2473734
display id: annotation2473734
version: 1
top level: BBa_K1600002
location: https://synbiohub.org/public/igem/BBa_K1600002/annotation2473734/range2473734/1
component: BBa_K322921

https://synbiohub.org/public/igem/BBa_K1600002/annotation2473734/range2473734/1
type: Range
persistent identity: range2473734
display id: range2473734
version: 1
top level: BBa_K1600002
direction: direction/reverse
start: 1369
end: 2805
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2473741
display id: annotation2473741
version: 1
top level: BBa_K1600002
location: https://synbiohub.org/public/igem/BBa_K1600002/annotation2473741/range2473741/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1600002/annotation2473741/range2473741/1
type: Range
persistent identity: range2473741
display id: range2473741
version: 1
top level: BBa_K1600002
direction: direction/reverse
start: 2814
end: 2942
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

BBa_K118016
type: ComponentDefinition
persistent identity: BBa K118016
display id: BBa_K118016
version: 1
was generated by: iGEM to SBOL conversion
description: glgC16 (glgC with G336D substitution)
created: 2008-10-05T11:00:00Z
modified: 2015-05-08T01:09:37Z
mutable provenance: ''Escherichia coli'' JM109 genomic DNA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the coding sequence of ''glgC'' (ADP-glucose pyrophosphorylase) from ''Escherichia coli'' JM109 with the substitution G336D. This mutation is known to cause increased activity of ADP-glucose pyrophosphorylase in the absence of the activator fructose 1,6-bisphosphate (FBP), high affinity for FBP and substrates lower affinity for the inhibitor AMP. (Leung ''et al'', 1986; Meyer ''et al.'', 1988)
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The mutation G336D was achieved by the substitution of A for G at position 1076 (codon GGC->GAC) by MABEL.
star: true
creator: Andrew Hall
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EcoRI site removed
sequence annotation: G336D
sequence: https://synbiohub.org/public/igem/BBa_K118016_sequence/1

EcoRI site removed
type: SequenceAnnotation
persistent identity: annotation1978558
display id: annotation1978558
version: 1
top level: BBa_K118016
location: https://synbiohub.org/public/igem/BBa_K118016/annotation1978558/range1978558/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K118016/annotation1978558/range1978558/1
type: Range
persistent identity: range1978558
display id: range1978558
version: 1
top level: BBa_K118016
direction: direction/forward
start: 571
end: 576
orientation: inline

G336D
type: SequenceAnnotation
persistent identity: annotation1978557
display id: annotation1978557
version: 1
top level: BBa_K118016
location: https://synbiohub.org/public/igem/BBa_K118016/annotation1978557/range1978557/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K118016/annotation1978557/range1978557/1
type: Range
persistent identity: range1978557
display id: range1978557
version: 1
top level: BBa_K118016
direction: direction/forward
start: 1006
end: 1008
orientation: inline

EcoRI site removed
type: SequenceAnnotation
persistent identity: annotation1978559
display id: annotation1978559
version: 1
top level: BBa_K118016
location: https://synbiohub.org/public/igem/BBa_K118016/annotation1978559/range1978559/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K118016/annotation1978559/range1978559/1
type: Range
persistent identity: range1978559
display id: range1978559
version: 1
top level: BBa_K118016
direction: direction/forward
start: 1068
end: 1073
orientation: inline

sacB
type: ComponentDefinition
persistent identity: BBa K322921
display id: BBa_K322921
version: 1
was generated by: iGEM to SBOL conversion
description: B. subtilis levansucrase. Lethal to E. coli in presence of sucrose.
created: 2010-09-23T11:00:00Z
modified: 2015-05-08T01:12:00Z
mutable provenance: Source: Bacillus subtilis 168 genomic DNA. Sequence (from SacBPlan11June10.docx)
owned by: james
owned by: myers
mutable description: sacB encodes the Bacillus subtilis levansucrase,which is the enzyme catalyzing hydrolysis of sucrose and synthesis of levans(high-molecular-weight fructose polymers). It is lethal to gram-negative bacteria E-coli. It works with cat as an alternative method for inserting BioBricks into the genome by using homologous recombination instead of restriction digestion, with the added bonus of not leaving a marker behind in the product. Protocol is shown here: http://2010.igem.org/Team:Edinburgh/Project/Protocol
discontinued: false
dominant: false
experience: experience/Works
group u list: _441_
m user id: 0
owner id: 6225
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Note: MABEL was used to mutate an internal EcoRI site to GAGTTC (underlined). Features: coding sequence runs from 13 to 1434, RBS 1 to 3, EcoRI site mutated 211 to 216.
star: false
creator: Chris French and Maria Kowal
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: RBS
sequence annotation: Coding sequence
sequence annotation: Removed EcoRI site
sequence: https://synbiohub.org/public/igem/BBa_K322921_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2098459
display id: annotation2098459
version: 1
top level: sacB
location: https://synbiohub.org/public/igem/BBa_K322921/annotation2098459/range2098459/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K322921/annotation2098459/range2098459/1
type: Range
persistent identity: range2098459
display id: range2098459
version: 1
top level: sacB
direction: direction/forward
start: 1
end: 3
orientation: inline

Coding sequence
type: SequenceAnnotation
persistent identity: annotation2098480
display id: annotation2098480
version: 1
top level: sacB
location: https://synbiohub.org/public/igem/BBa_K322921/annotation2098480/range2098480/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K322921/annotation2098480/range2098480/1
type: Range
persistent identity: range2098480
display id: range2098480
version: 1
top level: sacB
direction: direction/forward
start: 13
end: 1434
orientation: inline

Removed EcoRI site
type: SequenceAnnotation
persistent identity: annotation2098481
display id: annotation2098481
version: 1
top level: sacB
location: https://synbiohub.org/public/igem/BBa_K322921/annotation2098481/range2098481/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K322921/annotation2098481/range2098481/1
type: Range
persistent identity: range2098481
display id: range2098481
version: 1
top level: sacB
direction: direction/forward
start: 211
end: 216
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1600002_sequence/1
type: Sequence
persistent identity: BBa K1600002 sequence
display id: BBa_K1600002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggttagtttagagaagaacgatcacttaatgttggcgcgccagctgccattgaaatctgttgccctgatactggcgggaggacgtggtacccgcctgaaggatttaaccaataagcgagcaaaaccggccgtacacttcggcggtaagttccgcattatcgactttgcgctgtctaactgcatcaactccgggatccgtcgtatgggcgtgatcacccagtaccagtcccacactctggtgcagcacattcagcgcggctggtcattcttcaatgaagaaatgaacgagtttgtcgatctgctgccagcacagcagagaatgaaaggggaaaactggtatcgcggcaccgcagatgcggtcacccaaaacctcgacattatccgccgttataaagcggaatacgtggtgatcctggcgggcgaccatatctacaagcaagactactcgcgtatgcttatcgatcacgtcgaaaaaggcgcacgttgcaccgttgcttgtatgccagtaccgattgaagaagcctccgcatttggcgttatggcggttgatgagaacgataaaattatcgaatttgttgaaaaacctgctaacccgccgtcaatgccgaacgatccgagcaaatctctggcgagtatgggtatctacgtctttgacgccgactatctgtatgaactgctggaagaagacgatcgcgatgagaactccagccacgactttggcaaagatttgattcccaagatcaccgaagccggtctggcctatgcgcacccgttcccgctctcttgcgtacaatccgacccggatgccgagccgtactggcgcgatgtgggtacgctggaagcttactggaaagcgaacctcgatctggcctctgtggtgccggaactggatatgtacgatcgcaattggccaattcgcacctacaatgaatcattaccgccagcgaaattcgtgcaggatcgctccggtagccacgggatgacccttaactcactggtttccgacggttgtgtgatctccggttcggtggtggtgcagtccgttctgttctcgcgcgttcgcgtgaactcattctgcaacattgattccgccgtattgttaccggaagtatgggtaggtcgctcgtgccgtctgcgccgctgcgtcatcgatcgtgcttgtgttattccggaaggcatggtgattggtgaaaacgcagaggaagatgcacgtcgtttctatcgttcagaagaaggcatcgtgctggtaacgcgcgaaatgctacggaagttagggcataaacaggagcgataataatactagaggagacatgaacgatgaacatcaaaaagtttgcaaaacaagcaacagtattaacctttactaccgcactgctggcaggaggcgcaactcaagcgtttgcgaaagaaacgaaccaaaagccatataaggaaacatacggcatttcccatattacacgccatgatatgctgcaaatccctgaacagcaaaaaaatgaaaaatatcaagttcctgagttcgattcgtccacaattaaaaatatctcttctgcaaaaggcctggacgtttgggacagctggccattacaaaacgctgacggcactgtcgcaaactatcacggctaccacatcgtctttgcattagccggagatcctaaaaatgcggatgacacatcgatttacatgttctatcaaaaagtcggcgaaacttctattgacagctggaaaaacgctggccgcgtctttaaagacagcgacaaattcgatgcaaatgattctatcctaaaagaccaaacacaagaatggtcaggttcagccacatttacatctgacggaaaaatccgtttattctacactgatttctccggtaaacattacggcaaacaaacactgacaactgcacaagttaacgtatcagcatcagacagctctttgaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118016_sequence/1
type: Sequence
persistent identity: BBa K118016 sequence
display id: BBa_K118016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggttagtttagagaagaacgatcacttaatgttggcgcgccagctgccattgaaatctgttgccctgatactggcgggaggacgtggtacccgcctgaaggatttaaccaataagcgagcaaaaccggccgtacacttcggcggtaagttccgcattatcgactttgcgctgtctaactgcatcaactccgggatccgtcgtatgggcgtgatcacccagtaccagtcccacactctggtgcagcacattcagcgcggctggtcattcttcaatgaagaaatgaacgagtttgtcgatctgctgccagcacagcagagaatgaaaggggaaaactggtatcgcggcaccgcagatgcggtcacccaaaacctcgacattatccgccgttataaagcggaatacgtggtgatcctggcgggcgaccatatctacaagcaagactactcgcgtatgcttatcgatcacgtcgaaaaaggcgcacgttgcaccgttgcttgtatgccagtaccgattgaagaagcctccgcatttggcgttatggcggttgatgagaacgataaaattatcgaatttgttgaaaaacctgctaacccgccgtcaatgccgaacgatccgagcaaatctctggcgagtatgggtatctacgtctttgacgccgactatctgtatgaactgctggaagaagacgatcgcgatgagaactccagccacgactttggcaaagatttgattcccaagatcaccgaagccggtctggcctatgcgcacccgttcccgctctcttgcgtacaatccgacccggatgccgagccgtactggcgcgatgtgggtacgctggaagcttactggaaagcgaacctcgatctggcctctgtggtgccggaactggatatgtacgatcgcaattggccaattcgcacctacaatgaatcattaccgccagcgaaattcgtgcaggatcgctccggtagccacgggatgacccttaactcactggtttccgacggttgtgtgatctccggttcggtggtggtgcagtccgttctgttctcgcgcgttcgcgtgaactcattctgcaacattgattccgccgtattgttaccggaagtatgggtaggtcgctcgtgccgtctgcgccgctgcgtcatcgatcgtgcttgtgttattccggaaggcatggtgattggtgaaaacgcagaggaagatgcacgtcgtttctatcgttcagaagaaggcatcgtgctggtaacgcgcgaaatgctacggaagttagggcataaacaggagcgataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K322921_sequence/1
type: Sequence
persistent identity: BBa K322921 sequence
display id: BBa_K322921_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagacatgaacgatgaacatcaaaaagtttgcaaaacaagcaacagtattaacctttactaccgcactgctggcaggaggcgcaactcaagcgtttgcgaaagaaacgaaccaaaagccatataaggaaacatacggcatttcccatattacacgccatgatatgctgcaaatccctgaacagcaaaaaaatgaaaaatatcaagttcctgagttcgattcgtccacaattaaaaatatctcttctgcaaaaggcctggacgtttgggacagctggccattacaaaacgctgacggcactgtcgcaaactatcacggctaccacatcgtctttgcattagccggagatcctaaaaatgcggatgacacatcgatttacatgttctatcaaaaagtcggcgaaacttctattgacagctggaaaaacgctggccgcgtctttaaagacagcgacaaattcgatgcaaatgattctatcctaaaagaccaaacacaagaatggtcaggttcagccacatttacatctgacggaaaaatccgtttattctacactgatttctccggtaaacattacggcaaacaaacactgacaactgcacaagttaacgtatcagcatcagacagctctttgaacatcaacggtgtagaggattataaatcaatctttgacggtgacggaaaaacgtatcaaaatgtacagcagttcatcgatgaaggcaactacagctcaggcgacaaccatacgctgagagatcctcactacgtagaagataaaggccacaaatacttagtatttgaagcaaacactggaactgaagatggctaccaaggcgaagaatctttatttaacaaagcatactatggcaaaagcacatcattcttccgtcaagaaagtcaaaaacttctgcaaagcgataaaaaacgcacggctgagttagcaaacggcgctctcggtatgattgagctaaacgatgattacacactgaaaaaagtgatgaaaccgctgattgcatctaacacagtaacagatgaaattgaacgcgcgaacgtctttaaaatgaacggcaaatggtacctgttcactgactcccgcggatcaaaaatgacgattgacggcattacgtctaacgatatttacatgcttggttatgtttctaattctttaactggcccatacaagccgctgaacaaaactggccttgtgttaaaaatggatcttgatcctaacgatgtaacctttacttactcacacttcgctgtacctcaagcgaaaggaaacaatgtcgtgattacaagctatatgacaaacagaggattctacgcagacaaacaatcaacgtttgcgccaagcttcctgctgaacatcaaaggcaagaaaacatctgttgtcaaagacagcatccttgaacaaggacaattaacagttaacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z