LOCUS       BBa_K1600004            2940 bp    DNA     linear       22-Dec-2024
DEFINITION  Promoter+RBS+SacB+RBS+GlgC+Terminator
ACCESSION   BBa_K1600004
VERSION     BBa_K1600004.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23110
     misc_feature    44..46
                     /label=SacI
     RBS             47..53
                     /label=rbs
     RBS             44..53
                     /label=BBa_J15001
     misc_feature    1065..1067
                     /label=G336D
     misc_feature    630..635
                     /label=EcoRI site removed
     misc_feature    1127..1132
                     /label=EcoRI site removed
     CDS             60..1358
                     /label=BBa_K118016
     misc_feature    44..1358
                     /label=BBa_K118018
     RBS             1367..1369
                     /label=RBS
     CDS             1379..2800
                     /label=Coding sequence
     misc_feature    1577..1582
                     /label=Removed EcoRI site
     misc_feature    1367..2803
                     /label=BBa_K322921
     stem_loop       2823..2866
                     /label=stem_loop
     misc_feature    2812..2891
                     /label=BBa_B0010
     terminator      2812..2891
                     /label=BBa_B0010
     stem_loop       2907..2926
                     /label=T7 TE
     misc_feature    2933..2933
                     /label=stop
     polyA_site      2927..2940
                     /label=polya
     misc_feature    2900..2940
                     /label=BBa_B0012
     terminator      2900..2940
                     /label=BBa_B0012
     terminator      2812..2940
                     /label=BBa_B0015
ORIGIN
        1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagctcaagg aggtactaga
       61 tggttagttt agagaagaac gatcacttaa tgttggcgcg ccagctgcca ttgaaatctg
      121 ttgccctgat actggcggga ggacgtggta cccgcctgaa ggatttaacc aataagcgag
      181 caaaaccggc cgtacacttc ggcggtaagt tccgcattat cgactttgcg ctgtctaact
      241 gcatcaactc cgggatccgt cgtatgggcg tgatcaccca gtaccagtcc cacactctgg
      301 tgcagcacat tcagcgcggc tggtcattct tcaatgaaga aatgaacgag tttgtcgatc
      361 tgctgccagc acagcagaga atgaaagggg aaaactggta tcgcggcacc gcagatgcgg
      421 tcacccaaaa cctcgacatt atccgccgtt ataaagcgga atacgtggtg atcctggcgg
      481 gcgaccatat ctacaagcaa gactactcgc gtatgcttat cgatcacgtc gaaaaaggcg
      541 cacgttgcac cgttgcttgt atgccagtac cgattgaaga agcctccgca tttggcgtta
      601 tggcggttga tgagaacgat aaaattatcg aatttgttga aaaacctgct aacccgccgt
      661 caatgccgaa cgatccgagc aaatctctgg cgagtatggg tatctacgtc tttgacgccg
      721 actatctgta tgaactgctg gaagaagacg atcgcgatga gaactccagc cacgactttg
      781 gcaaagattt gattcccaag atcaccgaag ccggtctggc ctatgcgcac ccgttcccgc
      841 tctcttgcgt acaatccgac ccggatgccg agccgtactg gcgcgatgtg ggtacgctgg
      901 aagcttactg gaaagcgaac ctcgatctgg cctctgtggt gccggaactg gatatgtacg
      961 atcgcaattg gccaattcgc acctacaatg aatcattacc gccagcgaaa ttcgtgcagg
     1021 atcgctccgg tagccacggg atgaccctta actcactggt ttccgacggt tgtgtgatct
     1081 ccggttcggt ggtggtgcag tccgttctgt tctcgcgcgt tcgcgtgaac tcattctgca
     1141 acattgattc cgccgtattg ttaccggaag tatgggtagg tcgctcgtgc cgtctgcgcc
     1201 gctgcgtcat cgatcgtgct tgtgttattc cggaaggcat ggtgattggt gaaaacgcag
     1261 aggaagatgc acgtcgtttc tatcgttcag aagaaggcat cgtgctggta acgcgcgaaa
     1321 tgctacggaa gttagggcat aaacaggagc gataataata ctagaggaga catgaacgat
     1381 gaacatcaaa aagtttgcaa aacaagcaac agtattaacc tttactaccg cactgctggc
     1441 aggaggcgca actcaagcgt ttgcgaaaga aacgaaccaa aagccatata aggaaacata
     1501 cggcatttcc catattacac gccatgatat gctgcaaatc cctgaacagc aaaaaaatga
     1561 aaaatatcaa gttcctgagt tcgattcgtc cacaattaaa aatatctctt ctgcaaaagg
     1621 cctggacgtt tgggacagct ggccattaca aaacgctgac ggcactgtcg caaactatca
     1681 cggctaccac atcgtctttg cattagccgg agatcctaaa aatgcggatg acacatcgat
     1741 ttacatgttc tatcaaaaag tcggcgaaac ttctattgac agctggaaaa acgctggccg
     1801 cgtctttaaa gacagcgaca aattcgatgc aaatgattct atcctaaaag accaaacaca
     1861 agaatggtca ggttcagcca catttacatc tgacggaaaa atccgtttat tctacactga
     1921 tttctccggt aaacattacg gcaaacaaac actgacaact gcacaagtta acgtatcagc
     1981 atcagacagc tctttgaaca tcaacggtgt agaggattat aaatcaatct ttgacggtga
     2041 cggaaaaacg tatcaaaatg tacagcagtt catcgatgaa ggcaactaca gctcaggcga
     2101 caaccatacg ctgagagatc ctcactacgt agaagataaa ggccacaaat acttagtatt
     2161 tgaagcaaac actggaactg aagatggcta ccaaggcgaa gaatctttat ttaacaaagc
     2221 atactatggc aaaagcacat cattcttccg tcaagaaagt caaaaacttc tgcaaagcga
     2281 taaaaaacgc acggctgagt tagcaaacgg cgctctcggt atgattgagc taaacgatga
     2341 ttacacactg aaaaaagtga tgaaaccgct gattgcatct aacacagtaa cagatgaaat
     2401 tgaacgcgcg aacgtcttta aaatgaacgg caaatggtac ctgttcactg actcccgcgg
     2461 atcaaaaatg acgattgacg gcattacgtc taacgatatt tacatgcttg gttatgtttc
     2521 taattcttta actggcccat acaagccgct gaacaaaact ggccttgtgt taaaaatgga
     2581 tcttgatcct aacgatgtaa cctttactta ctcacacttc gctgtacctc aagcgaaagg
     2641 aaacaatgtc gtgattacaa gctatatgac aaacagagga ttctacgcag acaaacaatc
     2701 aacgtttgcg ccaagcttcc tgctgaacat caaaggcaag aaaacatctg ttgtcaaaga
     2761 cagcatcctt gaacaaggac aattaacagt taacaaataa taatactaga gccaggcatc
     2821 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     2881 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//