##gff-version 3
##sequence-region BBa_K1600004 1 2940
BBa_K1600004	.	terminator	2812	2940	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1600004	.	terminator	2812	2891	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1600004	.	engineered_region	2812	2891	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1600004	.	stem_loop	2823	2866	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1600004	.	terminator	2900	2940	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1600004	.	engineered_region	2900	2940	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1600004	.	stop_codon	2933	2933	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1600004	.	stem_loop	2907	2926	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1600004	.	polyA_site	2927	2940	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1600004	.	promoter	1	35	.	+	0	ID=BBa_J23110;Name=BBa_J23110
BBa_K1600004	.	engineered_region	1367	2803	.	+	0	ID=BBa_K322921;Name=BBa_K322921
BBa_K1600004	.	CDS	1379	2800	.	+	0	ID=annotation2098480;Name=Coding sequence;Parent=BBa_K322921
BBa_K1600004	.	point_mutation	1577	1582	.	+	0	ID=annotation2098481;Name=Removed EcoRI site;Parent=BBa_K322921
BBa_K1600004	.	ribosome_entry_site	1367	1369	.	+	0	ID=annotation2098459;Name=RBS;Parent=BBa_K322921
BBa_K1600004	.	engineered_region	44	1358	.	+	0	ID=BBa_K118018;Name=BBa_K118018
BBa_K1600004	.	ribosome_entry_site	44	53	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_K118018
BBa_K1600004	.	sequence_feature	44	46	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_K1600004	.	ribosome_entry_site	47	53	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_K1600004	.	CDS	60	1358	.	+	0	ID=BBa_K118016;Name=BBa_K118016;Parent=BBa_K118018
BBa_K1600004	.	sequence_alteration	1065	1067	.	+	0	ID=annotation1978557;Name=G336D;Parent=BBa_K118016
BBa_K1600004	.	point_mutation	1127	1132	.	+	0	ID=annotation1978559;Name=EcoRI site removed;Parent=BBa_K118016
BBa_K1600004	.	point_mutation	630	635	.	+	0	ID=annotation1978558;Name=EcoRI site removed;Parent=BBa_K118016
>BBa_K1600004
tttacggctagctcagtcctaggtacaatgctagctactagagctcaaggaggtactagatggttagtttagagaagaa
cgatcacttaatgttggcgcgccagctgccattgaaatctgttgccctgatactggcgggaggacgtggtacccgcctg
aaggatttaaccaataagcgagcaaaaccggccgtacacttcggcggtaagttccgcattatcgactttgcgctgtcta
actgcatcaactccgggatccgtcgtatgggcgtgatcacccagtaccagtcccacactctggtgcagcacattcagcg
cggctggtcattcttcaatgaagaaatgaacgagtttgtcgatctgctgccagcacagcagagaatgaaaggggaaaac
tggtatcgcggcaccgcagatgcggtcacccaaaacctcgacattatccgccgttataaagcggaatacgtggtgatcc
tggcgggcgaccatatctacaagcaagactactcgcgtatgcttatcgatcacgtcgaaaaaggcgcacgttgcaccgt
tgcttgtatgccagtaccgattgaagaagcctccgcatttggcgttatggcggttgatgagaacgataaaattatcgaa
tttgttgaaaaacctgctaacccgccgtcaatgccgaacgatccgagcaaatctctggcgagtatgggtatctacgtct
ttgacgccgactatctgtatgaactgctggaagaagacgatcgcgatgagaactccagccacgactttggcaaagattt
gattcccaagatcaccgaagccggtctggcctatgcgcacccgttcccgctctcttgcgtacaatccgacccggatgcc
gagccgtactggcgcgatgtgggtacgctggaagcttactggaaagcgaacctcgatctggcctctgtggtgccggaac
tggatatgtacgatcgcaattggccaattcgcacctacaatgaatcattaccgccagcgaaattcgtgcaggatcgctc
cggtagccacgggatgacccttaactcactggtttccgacggttgtgtgatctccggttcggtggtggtgcagtccgtt
ctgttctcgcgcgttcgcgtgaactcattctgcaacattgattccgccgtattgttaccggaagtatgggtaggtcgct
cgtgccgtctgcgccgctgcgtcatcgatcgtgcttgtgttattccggaaggcatggtgattggtgaaaacgcagagga
agatgcacgtcgtttctatcgttcagaagaaggcatcgtgctggtaacgcgcgaaatgctacggaagttagggcataaa
caggagcgataataatactagaggagacatgaacgatgaacatcaaaaagtttgcaaaacaagcaacagtattaacctt
tactaccgcactgctggcaggaggcgcaactcaagcgtttgcgaaagaaacgaaccaaaagccatataaggaaacatac
ggcatttcccatattacacgccatgatatgctgcaaatccctgaacagcaaaaaaatgaaaaatatcaagttcctgagt
tcgattcgtccacaattaaaaatatctcttctgcaaaaggcctggacgtttgggacagctggccattacaaaacgctga
cggcactgtcgcaaactatcacggctaccacatcgtctttgcattagccggagatcctaaaaatgcggatgacacatcg
atttacatgttctatcaaaaagtcggcgaaacttctattgacagctggaaaaacgctggccgcgtctttaaagacagcg
acaaattcgatgcaaatgattctatcctaaaagaccaaacacaagaatggtcaggttcagccacatttacatctgacgg
aaaaatccgtttattctacactgatttctccggtaaacattacggcaaacaaacactgacaactgcacaagttaacgta
tcagcatcagacagctctttgaacatcaacggtgtagaggattataaatcaatctttgacggtgacggaaaaacgtatc
aaaatgtacagcagttcatcgatgaaggcaactacagctcaggcgacaaccatacgctgagagatcctcactacgtaga
agataaaggccacaaatacttagtatttgaagcaaacactggaactgaagatggctaccaaggcgaagaatctttattt
aacaaagcatactatggcaaaagcacatcattcttccgtcaagaaagtcaaaaacttctgcaaagcgataaaaaacgca
cggctgagttagcaaacggcgctctcggtatgattgagctaaacgatgattacacactgaaaaaagtgatgaaaccgct
gattgcatctaacacagtaacagatgaaattgaacgcgcgaacgtctttaaaatgaacggcaaatggtacctgttcact
gactcccgcggatcaaaaatgacgattgacggcattacgtctaacgatatttacatgcttggttatgtttctaattctt
taactggcccatacaagccgctgaacaaaactggccttgtgttaaaaatggatcttgatcctaacgatgtaacctttac
ttactcacacttcgctgtacctcaagcgaaaggaaacaatgtcgtgattacaagctatatgacaaacagaggattctac
gcagacaaacaatcaacgtttgcgccaagcttcctgctgaacatcaaaggcaagaaaacatctgttgtcaaagacagca
tccttgaacaaggacaattaacagttaacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttata