BBa_K1601015 1 BBa_K1601015 T7 promoteur,RBS - Laccase T. Thermophilus - His Tag 2015-09-17T11:00:00Z 2015-09-18T05:59:22Z made with the biobricks Bba_K525998, BBa_K1601004 and Bba_K844000. T7 promoter with RBS and codon optimized Laccase from T. Thermophilus, protein likely to be more thermostable by its origin. A tag 10xHis is located to the end of the protein (Cterm) to enable its purification false false _2018_ 26607 26607 9 false no false Marion Aruanno component2474505 1 BBa_K844000 component2474501 1 BBa_K1601004 component2474500 1 BBa_K525998 annotation2474505 1 BBa_K844000 range2474505 1 1436 1471 annotation2474500 1 BBa_K525998 range2474500 1 1 32 annotation2474501 1 BBa_K1601004 range2474501 1 41 1427 BBa_K525998 1 pT7 Promoter T7 and RBS 2011-09-12T11:00:00Z 2015-05-08T01:12:35Z Synthesis Released HQ 2013 Promoter T7 and RBS false false _690_ 0 8543 9 In stock false Synthesis false Anna Drong annotation2127788 1 B0034 range2127788 1 21 32 annotation2129432 1 T7 promoter range2129432 1 1 20 annotation2129433 1 RBS range2129433 1 24 27 BBa_K844000 1 BBa_K844000 10x-Histidine (10x-His) Tag with double stop codon (TAATAA) 2012-10-01T11:00:00Z 2015-05-08T01:13:33Z Constructed through oligonucleotide annealing Released HQ 2013 10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part. false false _1104_ 0 9404 9 In stock true none false Kathleen Miller annotation2206607 1 10x-Histidine Tag range2206607 1 1 30 annotation2206609 1 Stop range2206609 1 34 36 annotation2206608 1 Stop range2206608 1 31 33 BBa_K1601004 1 BBa_K1601004 Laccase T.Thermophilus (Bba_K863011) without stop codon 2015-09-17T11:00:00Z 2015-09-18T05:37:05Z iGEM Laccase T.Thermophilus (Bba_K863011 from iGEM) without stop codon false false _2018_ 26607 26607 9 false We remove the stop codon using PCR false Marion Aruanno BBa_K525998_sequence 1 taatacgactcactatagggaaagaggagaaa BBa_K844000_sequence 1 catcatcaccatcaccaccatcatcaccattaataa BBa_K1601004_sequence 1 aatgctcgcacgccgttcctttctgcaagctgccgctggctcactggttttaggactggctcgggctcagggcccgtcatttccggagcccaaagttgtccgctcacagggtggcctgctgagcctgaagctgagtgccaccccgacaccgctggcgttggcaggtcagcgtgcgactctgttgacgtacggtggctcattccctggtccaaccctgcgtgtgcgtcctcgtgatacggtgcgcttaacactggaaaaccgtctgccagagcccacaaatctgcattggcacggtctgccgatttcgccgaaagtggatgatccgtttctggagatcccgccgggtgagtcctggacctacgaatttacggtgccgaaagaactggcgggaactttttggtaccacccgcacctgcacggtcgcgtagccccgcaactgtttgcgggtctgctgggcgccctcgttgtggagtcaagcctggatgctattccggagttacgcgaagccgaggagcacctgttggtgttaaaagatctggcgctgcaaggtggtcgtccggccccacacactccaatggactggatgaacggcaaagaaggcgacttagtcctggtcaatggcgcactgcgtccgacgctggtggcgcagaaggcgacgcttcgtctgcgtctgctgaacgcgagcaatgcgcggtattatcgcttggcactgcaagaccatccgctgtatctcatcgcggcggacggtggatttttagaggagcctctggaagtgtctgagctgctgctggccccgggtgaacgcgctgaggttttagtgcggcttcgtaaagagggccgctttttattacaggcgttaccatatgatcgcggggccatgggcatgatggatatgggtggcatggctcatgcaatgccgcaaggtccgagtcgcccggagacgctgctgtatctgatcgcgcccaaaaaccctaaaccactgccgcttccgaaagcgttgagcccatttcctactctcccggcgccggtcgtcacgcgtcgcctggtactgaccgaagacatgatggcggcgcgcttcttcattaacggtcaagtttttgaccatcgtcgtgtggatctgaaaggccaggcccagaccgtagaagtctgggaggttgaaaaccagggtgacatggaccaccctttccatctccatgtgcacccgtttcaagtgctgtccgtggggggccgtccatttccgtaccgcgcatggaaggacgtggttaacttgaaagccggcgaagttgcgcgcttgctggtccctttgcgcgagaaaggccgtaccgtttttcattgccatattgtggaacacgaggatcgtggcatgatgggtgttctggaagtgggc BBa_K1601015_sequence 1 taatacgactcactatagggaaagaggagaaatactagagaatgctcgcacgccgttcctttctgcaagctgccgctggctcactggttttaggactggctcgggctcagggcccgtcatttccggagcccaaagttgtccgctcacagggtggcctgctgagcctgaagctgagtgccaccccgacaccgctggcgttggcaggtcagcgtgcgactctgttgacgtacggtggctcattccctggtccaaccctgcgtgtgcgtcctcgtgatacggtgcgcttaacactggaaaaccgtctgccagagcccacaaatctgcattggcacggtctgccgatttcgccgaaagtggatgatccgtttctggagatcccgccgggtgagtcctggacctacgaatttacggtgccgaaagaactggcgggaactttttggtaccacccgcacctgcacggtcgcgtagccccgcaactgtttgcgggtctgctgggcgccctcgttgtggagtcaagcctggatgctattccggagttacgcgaagccgaggagcacctgttggtgttaaaagatctggcgctgcaaggtggtcgtccggccccacacactccaatggactggatgaacggcaaagaaggcgacttagtcctggtcaatggcgcactgcgtccgacgctggtggcgcagaaggcgacgcttcgtctgcgtctgctgaacgcgagcaatgcgcggtattatcgcttggcactgcaagaccatccgctgtatctcatcgcggcggacggtggatttttagaggagcctctggaagtgtctgagctgctgctggccccgggtgaacgcgctgaggttttagtgcggcttcgtaaagagggccgctttttattacaggcgttaccatatgatcgcggggccatgggcatgatggatatgggtggcatggctcatgcaatgccgcaaggtccgagtcgcccggagacgctgctgtatctgatcgcgcccaaaaaccctaaaccactgccgcttccgaaagcgttgagcccatttcctactctcccggcgccggtcgtcacgcgtcgcctggtactgaccgaagacatgatggcggcgcgcttcttcattaacggtcaagtttttgaccatcgtcgtgtggatctgaaaggccaggcccagaccgtagaagtctgggaggttgaaaaccagggtgacatggaccaccctttccatctccatgtgcacccgtttcaagtgctgtccgtggggggccgtccatttccgtaccgcgcatggaaggacgtggttaacttgaaagccggcgaagttgcgcgcttgctggtccctttgcgcgagaaaggccgtaccgtttttcattgccatattgtggaacacgaggatcgtggcatgatgggtgttctggaagtgggctactagagcatcatcaccatcaccaccatcatcaccattaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z