BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1602000
1
RBS-xylB
B0034-xylB
2015-08-27T11:00:00Z
2015-09-25T01:14:47Z
GenBank
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<partinfo>BBa_IA</partinfo>
<html>
<br>
<br>
<b>Itaconic acid</b> is an organic, dicarboxylic acid that is biotechnologically synthesized in Aspergillus terreus. It is derived from citric acid via 2 intermediates and a final decarboxylation.
<br>
To enable this pathway in Escherichia coli it is necessary to introduce 3 genes. 2 of which are already established in the citric acid cycle and one that is from Apergillus terreus, a cis-aconitate decarboxylase (cadA).
<img style="width: 900px; height: 99,4px; margin-left: 15px; margin-right: 15px;" alt=""
src="pictures/operon_ia.001.jpg">
<p style="width: 900px; height: 99,4px; margin-left: 15px; margin-right: 15px;" alt="" >
<br>
<b>Figure 1</b> Reaction scheme of the itaconic acid producing operon. The substrates for the reaction are acetyl-coa and oxaloacetate. To simplify the process we are only looking at oxaloacetate right now. Oxaloacetate is metabolized to itaconic acid in 3 steps.
</p>
<br>
<br>
===Usage===
<div align="right">
<table class="MsoTableGrid"
style="border: medium none ; border-collapse: collapse; text-align: left; margin-left: auto; margin-right: auto;"
border="0" cellpadding="0" cellspacing="0">
<tbody>
<tr style="height: 214.9pt;">
<td style="padding: 0cm 5.4pt; width: 336.7pt; height: 214.9pt;"> This part is a composite of three genes, each with a strong RBS (<a href="/Part:BBa_B0034">BBa_B0034</a>) and all of them under control of one T7 Promoter (<a href="/Part:BBa_K1497017">BBa_K1497017</a>).
<br>
<br>
<ul class="aufz10">
<li class="block-10vi">citrate synthase - gltA <a href="/Part:BBa_K1033001">(BBa_K1033001)</a></li>
<li class="block-10vi">aconitate hydratase 1 - acnA <a href="/Part:BBa_K1033000">(BBa_K1033000)</a></li>
<li class="block-10vi">cis-aconitate decarboxylase - cadA <a href="/Part:BBa_K1497000">(BBa_K1497100)</a></li>
</ul>
</td>
<td>
<font color="#FFFFFF">iGEM TU Darmstadt 2014 :)</font><br>
</td>
<td style="padding: 0cm 5.4pt; vertical-align: top; width: 236.7pt; height: 214.9pt;">
<img style="width: 400px; height: 120px;" alt="" src="pictures/all.png">
</p>
<p class="MsoCaption" align="text-align:justify">
<b>Figure 2</b>
Genetic map of the itaconic acid producing operon with T7 promoter. This brick directs the flux towards and finally enables <i>E.Coli</i> BL21 cells to synthesize itaconic acid in presence of the inductor IPTG.
<br>
</p>
</td>
</tr>
</tbody>
</table>
</div>
false
false
_2019_
12512
25989
9
Not in stock
false
BLANK
false
Jonas Sindlinger, Leon Kraus, Jonathan Pletzer-Zelgert, Elena Tonchevska, Sebastian Holzner, Mirjam Haun, Sebastian Barthel
component2463049
1
BBa_B0034
component2463050
1
BBa_K1602009
annotation2463049
1
BBa_B0034
range2463049
1
1
12
annotation2463050
1
BBa_K1602009
range2463050
1
19
765
BBa_K1602009
1
BBa_K1602009
xylB
2015-09-10T11:00:00Z
2015-09-25T01:22:39Z
BLANK
BLANK
false
false
_2019_
12512
25989
9
false
BLANK
false
Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
BBa_K1602000_sequence
1
aaagaggagaaatactagatgagcagcgccatttatccgagcctgaaaggtaaacgtgttgttattacaggtggtggtagcggtattggtgcaggtctgaccgcaggttttgcacgtcagggtgcagaagttatttttctggatattgcagatgaagatagccgtgcactggaagcagaactggcaggtagcccgattccgcctgtgtataaacgttgtgatctgatgaatctggaagccattaaagcagtgtttgccgaaattggtgatgttgatgttctggttaataacgcaggtaatgatgatcgtcataaactggcagatgttacaggtgcatattgggatgaacgcattaatgttaatctgcgccacatgctgttttgtacccaggcagttgcaccgggtatgaaaaaacgtggtggtggtgcagttattaactttggtagcattagctggcatctgggtttagaggatctggttctgtatgaaaccgcaaaagcaggtattgaaggtatgacccgtgccctggcacgtgaactgggtccggatgatattcgtgttacctgtgttgttccgggtaatgttaaaaccaaacgccaagagaaatggtatacaccggaaggtgaagcacagattgttgcagcacagtgtctgaaaggtcgtattgttccggaaaatgttgcagccctggtgctgtttctggcaagtgatgatgcaagcctgtgtacaggtcatgaatattggattgatgcaggttggcgttaa
BBa_K1602009_sequence
1
atgagcagcgccatttatccgagcctgaaaggtaaacgtgttgttattacaggtggtggtagcggtattggtgcaggtctgaccgcaggttttgcacgtcagggtgcagaagttatttttctggatattgcagatgaagatagccgtgcactggaagcagaactggcaggtagcccgattccgcctgtgtataaacgttgtgatctgatgaatctggaagccattaaagcagtgtttgccgaaattggtgatgttgatgttctggttaataacgcaggtaatgatgatcgtcataaactggcagatgttacaggtgcatattgggatgaacgcattaatgttaatctgcgccacatgctgttttgtacccaggcagttgcaccgggtatgaaaaaacgtggtggtggtgcagttattaactttggtagcattagctggcatctgggtttagaggatctggttctgtatgaaaccgcaaaagcaggtattgaaggtatgacccgtgccctggcacgtgaactgggtccggatgatattcgtgttacctgtgttgttccgggtaatgttaaaaccaaacgccaagagaaatggtatacaccggaaggtgaagcacagattgttgcagcacagtgtctgaaaggtcgtattgttccggaaaatgttgcagccctggtgctgtttctggcaagtgatgatgcaagcctgtgtacaggtcatgaatattggattgatgcaggttggcgttaa
BBa_B0034_sequence
1
aaagaggagaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z