BBa_K1602057
type: ComponentDefinition
persistent identity: BBa K1602057
display id: BBa_K1602057
version: 1
was generated by: iGEM to SBOL conversion
description: T7-B0034-yagE-B0034-yjhG-B0034-yqhDB0034-yagE-B0034-yjhG-B0034-yqhD
created: 2015-09-15T11:00:00Z
modified: 2015-09-18T07:31:45Z
owned by: james
owned by: myers
discontinued: true
dominant: false
experience: experience/None
group u list: _2019_
m user id: 25990
owner id: 25990
owning group id: 9
status: status/Deleted
bookmark: false
star: false
creator: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1602002
component: BBa_K1602001
component: BBa_K1602000
component: BBa_K1602013
component: BBa_B0034
component: BBa_K1602014
sequence annotation: BBa_K1602000
sequence annotation: BBa_K1602001
sequence annotation: BBa_K1602002
sequence annotation: BBa_K1602014
sequence annotation: BBa_B0034
sequence annotation: BBa_K1602013
sequence: https://synbiohub.org/public/igem/BBa_K1602057_sequence/1

BBa_K1602002
type: Component
persistent identity: component2463107
display id: component2463107
version: 1
top level: BBa_K1602057
definition: RBS-yjhG
access: public

BBa_K1602001
type: Component
persistent identity: component2463103
display id: component2463103
version: 1
top level: BBa_K1602057
definition: RBS-xylC
access: public

BBa_K1602000
type: Component
persistent identity: component2463099
display id: component2463099
version: 1
top level: BBa_K1602057
definition: RBS-xylB
access: public

BBa_K1602013
type: Component
persistent identity: component2463114
display id: component2463114
version: 1
top level: BBa_K1602057
definition: BBa_K1602013
access: public

BBa_B0034
type: Component
persistent identity: component2463112
display id: component2463112
version: 1
top level: BBa_K1602057
definition: BBa_B0034
access: public

BBa_K1602014
type: Component
persistent identity: component2463111
display id: component2463111
version: 1
top level: BBa_K1602057
definition: BBa_K1602014
access: public

BBa_K1602000
type: SequenceAnnotation
persistent identity: annotation2463099
display id: annotation2463099
version: 1
top level: BBa_K1602057
location: https://synbiohub.org/public/igem/BBa_K1602057/annotation2463099/range2463099/1
component: BBa_K1602000

https://synbiohub.org/public/igem/BBa_K1602057/annotation2463099/range2463099/1
type: Range
persistent identity: range2463099
display id: range2463099
version: 1
top level: BBa_K1602057
direction: direction/reverse
start: 1
end: 765
orientation: inline

BBa_K1602001
type: SequenceAnnotation
persistent identity: annotation2463103
display id: annotation2463103
version: 1
top level: BBa_K1602057
location: https://synbiohub.org/public/igem/BBa_K1602057/annotation2463103/range2463103/1
component: BBa_K1602001

https://synbiohub.org/public/igem/BBa_K1602057/annotation2463103/range2463103/1
type: Range
persistent identity: range2463103
display id: range2463103
version: 1
top level: BBa_K1602057
direction: direction/reverse
start: 774
end: 1661
orientation: inline

BBa_K1602002
type: SequenceAnnotation
persistent identity: annotation2463107
display id: annotation2463107
version: 1
top level: BBa_K1602057
location: https://synbiohub.org/public/igem/BBa_K1602057/annotation2463107/range2463107/1
component: BBa_K1602002

https://synbiohub.org/public/igem/BBa_K1602057/annotation2463107/range2463107/1
type: Range
persistent identity: range2463107
display id: range2463107
version: 1
top level: BBa_K1602057
direction: direction/reverse
start: 1670
end: 3655
orientation: inline

BBa_K1602014
type: SequenceAnnotation
persistent identity: annotation2463111
display id: annotation2463111
version: 1
top level: BBa_K1602057
location: https://synbiohub.org/public/igem/BBa_K1602057/annotation2463111/range2463111/1
component: BBa_K1602014

https://synbiohub.org/public/igem/BBa_K1602057/annotation2463111/range2463111/1
type: Range
persistent identity: range2463111
display id: range2463111
version: 1
top level: BBa_K1602057
direction: direction/reverse
start: 3664
end: 4590
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2463112
display id: annotation2463112
version: 1
top level: BBa_K1602057
location: https://synbiohub.org/public/igem/BBa_K1602057/annotation2463112/range2463112/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602057/annotation2463112/range2463112/1
type: Range
persistent identity: range2463112
display id: range2463112
version: 1
top level: BBa_K1602057
direction: direction/reverse
start: 4599
end: 4610
orientation: inline

BBa_K1602013
type: SequenceAnnotation
persistent identity: annotation2463114
display id: annotation2463114
version: 1
top level: BBa_K1602057
location: https://synbiohub.org/public/igem/BBa_K1602057/annotation2463114/range2463114/1
component: BBa_K1602013

https://synbiohub.org/public/igem/BBa_K1602057/annotation2463114/range2463114/1
type: Range
persistent identity: range2463114
display id: range2463114
version: 1
top level: BBa_K1602057
direction: direction/reverse
start: 4617
end: 5780
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_K1602013
type: ComponentDefinition
persistent identity: BBa K1602013
display id: BBa_K1602013
version: 1
was generated by: iGEM to SBOL conversion
description: yqhD
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T06:39:43Z
mutable provenance: BLANK
owned by: james
owned by: myers
mutable description: BLANK
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 26220
owner id: 25878
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: BLANK
star: false
creator: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1602013_sequence/1

BBa_K1602014
type: ComponentDefinition
persistent identity: BBa K1602014
display id: BBa_K1602014
version: 1
was generated by: iGEM to SBOL conversion
description: B0034-yagE
created: 2015-09-15T11:00:00Z
modified: 2015-09-18T07:26:18Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 25990
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1602011
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1602011
sequence: https://synbiohub.org/public/igem/BBa_K1602014_sequence/1

BBa_K1602011
type: Component
persistent identity: component2462504
display id: component2462504
version: 1
top level: BBa_K1602014
definition: BBa_K1602011
access: public

BBa_B0034
type: Component
persistent identity: component2462503
display id: component2462503
version: 1
top level: BBa_K1602014
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2462503
display id: annotation2462503
version: 1
top level: BBa_K1602014
location: https://synbiohub.org/public/igem/BBa_K1602014/annotation2462503/range2462503/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602014/annotation2462503/range2462503/1
type: Range
persistent identity: range2462503
display id: range2462503
version: 1
top level: BBa_K1602014
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1602011
type: SequenceAnnotation
persistent identity: annotation2462504
display id: annotation2462504
version: 1
top level: BBa_K1602014
location: https://synbiohub.org/public/igem/BBa_K1602014/annotation2462504/range2462504/1
component: BBa_K1602011

https://synbiohub.org/public/igem/BBa_K1602014/annotation2462504/range2462504/1
type: Range
persistent identity: range2462504
display id: range2462504
version: 1
top level: BBa_K1602014
direction: direction/reverse
start: 19
end: 927
orientation: inline

RBS-xylB
type: ComponentDefinition
persistent identity: BBa K1602000
display id: BBa_K1602000
version: 1
was generated by: iGEM to SBOL conversion
description: B0034-xylB
created: 2015-08-27T11:00:00Z
modified: 2015-09-25T01:14:47Z
mutable provenance: GenBank
owned by: james
owned by: myers
mutable description: <partinfo>BBa_IA</partinfo> Itaconic acid is an organic, dicarboxylic acid that is biotechnologically synthesized in Aspergillus terreus. It is derived from citric acid via 2 intermediates and a final decarboxylation. To enable this pathway in Escherichia coli it is necessary to introduce 3 genes. 2 of which are already established in the citric acid cycle and one that is from Apergillus terreus, a cis-aconitate decarboxylase (cadA). Figure 1 Reaction scheme of the itaconic acid producing operon. The substrates for the reaction are acetyl-coa and oxaloacetate. To simplify the process we are only looking at oxaloacetate right now. Oxaloacetate is metabolized to itaconic acid in 3 steps. ===Usage=== This part is a composite of three genes, each with a strong RBS (BBa_B0034) and all of them under control of one T7 Promoter (BBa_K1497017). citrate synthase - gltA (BBa_K1033001) aconitate hydratase 1 - acnA (BBa_K1033000) cis-aconitate decarboxylase - cadA (BBa_K1497100) iGEM TU Darmstadt 2014 :) Figure 2 Genetic map of the itaconic acid producing operon with T7 promoter. This brick directs the flux towards and finally enables E.Coli BL21 cells to synthesize itaconic acid in presence of the inductor IPTG.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2019_
m user id: 12512
owner id: 25989
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: BLANK
star: false
creator: Jonas Sindlinger, Leon Kraus, Jonathan Pletzer-Zelgert, Elena Tonchevska, Sebastian Holzner, Mirjam Haun, Sebastian Barthel
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1602009
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1602009
sequence: https://synbiohub.org/public/igem/BBa_K1602000_sequence/1

BBa_K1602009
type: Component
persistent identity: component2463050
display id: component2463050
version: 1
top level: RBS-xylB
definition: BBa_K1602009
access: public

BBa_B0034
type: Component
persistent identity: component2463049
display id: component2463049
version: 1
top level: RBS-xylB
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2463049
display id: annotation2463049
version: 1
top level: RBS-xylB
location: https://synbiohub.org/public/igem/BBa_K1602000/annotation2463049/range2463049/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602000/annotation2463049/range2463049/1
type: Range
persistent identity: range2463049
display id: range2463049
version: 1
top level: RBS-xylB
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1602009
type: SequenceAnnotation
persistent identity: annotation2463050
display id: annotation2463050
version: 1
top level: RBS-xylB
location: https://synbiohub.org/public/igem/BBa_K1602000/annotation2463050/range2463050/1
component: BBa_K1602009

https://synbiohub.org/public/igem/BBa_K1602000/annotation2463050/range2463050/1
type: Range
persistent identity: range2463050
display id: range2463050
version: 1
top level: RBS-xylB
direction: direction/reverse
start: 19
end: 765
orientation: inline

RBS-xylC
type: ComponentDefinition
persistent identity: BBa K1602001
display id: BBa_K1602001
version: 1
was generated by: iGEM to SBOL conversion
description: B0034-xylC
created: 2015-08-27T11:00:00Z
modified: 2015-09-18T12:11:36Z
mutable provenance: E.coli TOP10
owned by: james
owned by: myers
mutable description: Blank
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 22022
owner id: 25989
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Blank
star: false
creator: Jonas Sindlinger, Leon Kraus, Jonathan Pletzer-Zelgert, Elena Tonchevska, Sebastian Holzner, Mirjam Haun, Sebastian Barthel
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
component: BBa_K1602010
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1602010
sequence: https://synbiohub.org/public/igem/BBa_K1602001_sequence/1

BBa_K1602010
type: Component
persistent identity: component2463053
display id: component2463053
version: 1
top level: RBS-xylC
definition: BBa_K1602010
access: public

BBa_B0034
type: Component
persistent identity: component2463052
display id: component2463052
version: 1
top level: RBS-xylC
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2463052
display id: annotation2463052
version: 1
top level: RBS-xylC
location: https://synbiohub.org/public/igem/BBa_K1602001/annotation2463052/range2463052/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602001/annotation2463052/range2463052/1
type: Range
persistent identity: range2463052
display id: range2463052
version: 1
top level: RBS-xylC
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1602010
type: SequenceAnnotation
persistent identity: annotation2463053
display id: annotation2463053
version: 1
top level: RBS-xylC
location: https://synbiohub.org/public/igem/BBa_K1602001/annotation2463053/range2463053/1
component: BBa_K1602010

https://synbiohub.org/public/igem/BBa_K1602001/annotation2463053/range2463053/1
type: Range
persistent identity: range2463053
display id: range2463053
version: 1
top level: RBS-xylC
direction: direction/reverse
start: 19
end: 888
orientation: inline

RBS-yjhG
type: ComponentDefinition
persistent identity: BBa K1602002
display id: BBa_K1602002
version: 1
was generated by: iGEM to SBOL conversion
description: B0034-yjhG
created: 2015-08-27T11:00:00Z
modified: 2015-09-18T12:10:35Z
mutable provenance: E.coli TOP10
owned by: james
owned by: myers
mutable description: Blank
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 22022
owner id: 25989
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Blank
star: false
creator: Jonas Sindlinger, Leon Kraus, Jonathan Pletzer-Zelgert, Elena Tonchevska, Sebastian Holzner, Mirjam Haun, Sebastian Barthel
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
component: BBa_K1602012
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1602012
sequence: https://synbiohub.org/public/igem/BBa_K1602002_sequence/1

BBa_K1602012
type: Component
persistent identity: component2463058
display id: component2463058
version: 1
top level: RBS-yjhG
definition: BBa_K1602012
access: public

BBa_B0034
type: Component
persistent identity: component2463057
display id: component2463057
version: 1
top level: RBS-yjhG
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2463057
display id: annotation2463057
version: 1
top level: RBS-yjhG
location: https://synbiohub.org/public/igem/BBa_K1602002/annotation2463057/range2463057/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1602002/annotation2463057/range2463057/1
type: Range
persistent identity: range2463057
display id: range2463057
version: 1
top level: RBS-yjhG
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1602012
type: SequenceAnnotation
persistent identity: annotation2463058
display id: annotation2463058
version: 1
top level: RBS-yjhG
location: https://synbiohub.org/public/igem/BBa_K1602002/annotation2463058/range2463058/1
component: BBa_K1602012

https://synbiohub.org/public/igem/BBa_K1602002/annotation2463058/range2463058/1
type: Range
persistent identity: range2463058
display id: range2463058
version: 1
top level: RBS-yjhG
direction: direction/reverse
start: 19
end: 1986
orientation: inline

BBa_K1602010
type: ComponentDefinition
persistent identity: BBa K1602010
display id: BBa_K1602010
version: 1
was generated by: iGEM to SBOL conversion
description: xylC
created: 2015-09-13T11:00:00Z
modified: 2015-09-25T01:32:15Z
mutable provenance: BLANK
owned by: james
owned by: myers
mutable description: BLANK
discontinued: false
dominant: false
experience: experience/Works
group u list: _2019_
m user id: 12512
owner id: 25878
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: BLANK
star: false
creator: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1602010_sequence/1

BBa_K1602011
type: ComponentDefinition
persistent identity: BBa K1602011
display id: BBa_K1602011
version: 1
was generated by: iGEM to SBOL conversion
description: yagE
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T06:39:03Z
mutable provenance: BLANK
owned by: james
owned by: myers
mutable description: BLANK
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 26220
owner id: 25878
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: BLANK
star: false
creator: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1602011_sequence/1

BBa_K1602012
type: ComponentDefinition
persistent identity: BBa K1602012
display id: BBa_K1602012
version: 1
was generated by: iGEM to SBOL conversion
description: yjhG
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T06:39:23Z
mutable provenance: BLANK
owned by: james
owned by: myers
mutable description: BLANK
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 26220
owner id: 25878
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: BLANK
star: false
creator: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1602012_sequence/1

BBa_K1602009
type: ComponentDefinition
persistent identity: BBa K1602009
display id: BBa_K1602009
version: 1
was generated by: iGEM to SBOL conversion
description: xylB
created: 2015-09-10T11:00:00Z
modified: 2015-09-25T01:22:39Z
mutable provenance: BLANK
owned by: james
owned by: myers
mutable description: BLANK
discontinued: false
dominant: false
experience: experience/Works
group u list: _2019_
m user id: 12512
owner id: 25989
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: BLANK
star: false
creator: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1602009_sequence/1

https://synbiohub.org/public/igem/BBa_K1602057_sequence/1
type: Sequence
persistent identity: BBa K1602057 sequence
display id: BBa_K1602057_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagcagcgccatttatccgagcctgaaaggtaaacgtgttgttattacaggtggtggtagcggtattggtgcaggtctgaccgcaggttttgcacgtcagggtgcagaagttatttttctggatattgcagatgaagatagccgtgcactggaagcagaactggcaggtagcccgattccgcctgtgtataaacgttgtgatctgatgaatctggaagccattaaagcagtgtttgccgaaattggtgatgttgatgttctggttaataacgcaggtaatgatgatcgtcataaactggcagatgttacaggtgcatattgggatgaacgcattaatgttaatctgcgccacatgctgttttgtacccaggcagttgcaccgggtatgaaaaaacgtggtggtggtgcagttattaactttggtagcattagctggcatctgggtttagaggatctggttctgtatgaaaccgcaaaagcaggtattgaaggtatgacccgtgccctggcacgtgaactgggtccggatgatattcgtgttacctgtgttgttccgggtaatgttaaaaccaaacgccaagagaaatggtatacaccggaaggtgaagcacagattgttgcagcacagtgtctgaaaggtcgtattgttccggaaaatgttgcagccctggtgctgtttctggcaagtgatgatgcaagcctgtgtacaggtcatgaatattggattgatgcaggttggcgttaatactagagaaagaggagaaatactagatgaccgcacaggttacctgcgtttgggatctgaaagcaaccctgggtgaaggtccgatttggcatggtgataccctgtggtttgttgatattaaacagcgcaaaatccacaactatcatccggcaacaggtgaacgttttagctttgatgcaccggatcaggtgacctttctggcaccgattgttggtgcaaccggctttgttgttggtctgaaaaccggcattcatcgttttcatcctgccaccggctttagcctgctgctggaagttgaagatgcagcactgaataatcgtccgaatgatgcaaccgttgatgcacagggtcgtctgtggttcggcaccatgcatgatggcgaagaaaataacagcggtagcctgtatcgtatggatctgacaggtgttgcacgcatggatcgtgatatttgtattaccaatggtccgtgtgttagtccggatggtaaaaccttttatcataccgataccctggaaaaaaccatctatgcatttgatctggcagaagatggtctgctgagcaataaacgtgtttttgttcagtttgcactgggtgatgatgtttacccggatggttcagttgttgatagcgaaggttatctgtggaccgcactgtggggtggttttggtgcagttcgttttagtccgcagggtgatgcagttacccgtattgaactgcctgcaccgaatgttaccaaaccgtgttttggtggtcctgatctgaaaacactgtattttaccaccgcacgtaaaggtctgagtgatgaaaccctggcacagtatccgctggcaggcggtgtttttgcagttccggttgatgttgcaggtcagccgcagcatgaagttcgtctggtttaatactagagaaagaggagaaatactagatgtctgttcgcaatatttttgctgacgagagccacgatatttacaccgtcagaacgcacgccgatggcccggacggcgaactcccattaaccgcagagatgcttatcaaccgcccgagcggggatctgttcggtatgaccatgaatgccggaatgggttggtctccggacgagctggatcgggacggtattttactgctcagtacactcggtggcttacgcggcgcagacggtaaacccgtggcgctggcgttgcaccaggggcattacgaactggacatccagatgaaagcggcggccgaggttattaaag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1602013_sequence/1
type: Sequence
persistent identity: BBa K1602013 sequence
display id: BBa_K1602013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacaactttaatctgcacaccccaacccgcattctgtttggtaaaggcgcaatcgctggtttacgcgaacaaattcctcacgatgctcgcgtattgattacctacggcggcggcagcgtgaaaaaaaccggcgttctcgatcaagttctggatgccctgaaaggcatggacgtgctggaatttggcggtattgagccaaacccggcttatgaaacgctgatgaacgccgtgaaactggttcgcgaacagaaagtgactttcctgctggcggttggcggcggttctgtactggacggcaccaaatttatcgccgcagcggctaactatccggaaaatatcgatccgtggcacattctgcaaacgggcggtaaagagattaaaagcgccatcccgatgggctgtgtgctgacgctgccagcaaccggttcagaatccaacgcaggcgcggtgatctcccgtaaaaccacaggcgacaagcaggcgttccattctgcccatgttcagccggtatttgccgtgctcgatccggtttatacctacaccctgccgccgcgtcaggtggctaacggcgtagtggacgcctttgtacacaccgtggaacagtatgttaccaaaccggttgatgccaaaattcaggaccgtttcgcagaaggcattttgctgacgctaatcgaagatggtccgaaagccctgaaagagccagaaaactacgatgtgcgcgccaacgtcatgtgggcggcgactcaggcgctgaacggtttgattggcgctggcgtaccgcaggactgggcaacgcatatgctgggccacgaactgactgcgatgcacggtctggatcacgcgcaaacactggctatcgtcctgcctgcactgtggaatgaaaaacgcgataccaagcgcgctaagctgctgcaatatgctgaacgcgtctggaacatcactgaaggttccgatgatgagcgtattgacgccgcgattgccgcaacccgcaatttctttgagcaattaggcgtgccgacccacctctccgactacggtctggacggcagctccatcccggctttgctgaaaaaactggaagagcacggcatgacccaactgggcgaaaatcatgacattacgttggatgtcagccgccgtatatacgaagccgcccgctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1602014_sequence/1
type: Sequence
persistent identity: BBa K1602014 sequence
display id: BBa_K1602014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgccgcagtccgcgttgttcacgggaatcattccccctgtctccaccatttttaccgccgacggccagctcgataagccgggcaccgccgcgctgatcgacgatctgatcaaagcaggcgttgacggcctgttcttcctgggcagcggtggcgagttctcccagctcggcgccgaagagcgtaaagccattgcccgctttgctatcgatcatgtcgatcgtcgcgtgccggtgctgatcggcaccggcggcaccaacgcccgggaaaccatcgaactcagccagcacgcgcagcaggcgggcgcggacggcatcgtggtgatcaacccctactactggaaagtgtcggaagcgaacctgatccgctatttcgagcaggtggccgacagcgtcacgctgccggtgatgctctataacttcccggcgctgaccgggcaggatctgactccggcgctggtgaaaaccctcgccgactcgcgcagcaatattatcggcatcaaagacaccatcgactccgtcgcccacctgcgcagcatgatccataccgtcaaaggtgcccatccgcacttcaccgtgctctgcggctacgacgatcatctgttcaataccctgctgctcggcggcgacggggcgatatcggcgagcggcaactttgccccgcaggtgtcggtgaatcttctgaaagcctggcgcgacggggacgtggcgaaagcggccgggtatcatcagaccttgctgcaaattccgcagatgtatcagctggatacgccgtttgtgaacgtgattaaagaggcgatcgtgctctgcggtcgtcctgtctccacgcacgtgctgccgcccgcctcgccgctggacgagccgcgcaaggcgcagctgaaaaccctgctgcaacagctcaagctttgctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1602000_sequence/1
type: Sequence
persistent identity: BBa K1602000 sequence
display id: BBa_K1602000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagcagcgccatttatccgagcctgaaaggtaaacgtgttgttattacaggtggtggtagcggtattggtgcaggtctgaccgcaggttttgcacgtcagggtgcagaagttatttttctggatattgcagatgaagatagccgtgcactggaagcagaactggcaggtagcccgattccgcctgtgtataaacgttgtgatctgatgaatctggaagccattaaagcagtgtttgccgaaattggtgatgttgatgttctggttaataacgcaggtaatgatgatcgtcataaactggcagatgttacaggtgcatattgggatgaacgcattaatgttaatctgcgccacatgctgttttgtacccaggcagttgcaccgggtatgaaaaaacgtggtggtggtgcagttattaactttggtagcattagctggcatctgggtttagaggatctggttctgtatgaaaccgcaaaagcaggtattgaaggtatgacccgtgccctggcacgtgaactgggtccggatgatattcgtgttacctgtgttgttccgggtaatgttaaaaccaaacgccaagagaaatggtatacaccggaaggtgaagcacagattgttgcagcacagtgtctgaaaggtcgtattgttccggaaaatgttgcagccctggtgctgtttctggcaagtgatgatgcaagcctgtgtacaggtcatgaatattggattgatgcaggttggcgttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1602001_sequence/1
type: Sequence
persistent identity: BBa K1602001 sequence
display id: BBa_K1602001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaccgcacaggttacctgcgtttgggatctgaaagcaaccctgggtgaaggtccgatttggcatggtgataccctgtggtttgttgatattaaacagcgcaaaatccacaactatcatccggcaacaggtgaacgttttagctttgatgcaccggatcaggtgacctttctggcaccgattgttggtgcaaccggctttgttgttggtctgaaaaccggcattcatcgttttcatcctgccaccggctttagcctgctgctggaagttgaagatgcagcactgaataatcgtccgaatgatgcaaccgttgatgcacagggtcgtctgtggttcggcaccatgcatgatggcgaagaaaataacagcggtagcctgtatcgtatggatctgacaggtgttgcacgcatggatcgtgatatttgtattaccaatggtccgtgtgttagtccggatggtaaaaccttttatcataccgataccctggaaaaaaccatctatgcatttgatctggcagaagatggtctgctgagcaataaacgtgtttttgttcagtttgcactgggtgatgatgtttacccggatggttcagttgttgatagcgaaggttatctgtggaccgcactgtggggtggttttggtgcagttcgttttagtccgcagggtgatgcagttacccgtattgaactgcctgcaccgaatgttaccaaaccgtgttttggtggtcctgatctgaaaacactgtattttaccaccgcacgtaaaggtctgagtgatgaaaccctggcacagtatccgctggcaggcggtgtttttgcagttccggttgatgttgcaggtcagccgcagcatgaagttcgtctggtttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1602002_sequence/1
type: Sequence
persistent identity: BBa K1602002 sequence
display id: BBa_K1602002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtctgttcgcaatatttttgctgacgagagccacgatatttacaccgtcagaacgcacgccgatggcccggacggcgaactcccattaaccgcagagatgcttatcaaccgcccgagcggggatctgttcggtatgaccatgaatgccggaatgggttggtctccggacgagctggatcgggacggtattttactgctcagtacactcggtggcttacgcggcgcagacggtaaacccgtggcgctggcgttgcaccaggggcattacgaactggacatccagatgaaagcggcggccgaggttattaaagccaaccatgccctgccctatgccgtgtacgtctccgatccttgtgacgggcgtactcagggtacaacggggatgtttgattcgctaccataccgaaatgacgcatcgatggtaatgcgccgccttattcgctctctgcccgacgcgaaagcagttattggtgtggcgagttgcgataaggggcttccggccaccatgatggcactcgccgcgcagcacaacatcgcaaccgtgctggtccccggcggcgcgacgctgcccgcaaaggatggagaagacaacggcaaggtgcaaaccattggcgcacgcttcgccaatggcgaattatctctacaggacgcacgccgtgcgggctgtaaagcctgtgcctcttccggcggcggctgtcaatttttgggcactgccgggacatctcaggtggtggccgaaggattgggactggcaatcccacattcagccctggccccttccggtgagcctgtgtggcgggagatcgccagagcttccgcgcgagctgcgctgaacctgagtcaaaaaggcatcaccacccgggaaattctcaccgataaagcgatagagaatgcgatgacggtccatgccgcgttcggtggttcaacaaacctgctgttacacatcccggcaattgctcaccaggcaggttgccatatcccgaccgttgatgactggatccgcatcaacaagcgcgtgccccgactggtgagcgtactgcctaatggcccggtttatcatccaacggtcaatgcctttatggcaggtggtgtgccggaagtcatgttgcatctgcgcagcctcggattgttgcatgaagacgttatgacggttaccggcagcacgctgaaagaaaacctcgactggtgggagcactccgaacggcgtcagcggttcaagcaactcctgctcgatcaggaacaaatcaacgctgacgaagtgatcatgtctccgcagcaagcaaaagcgcgcggattaacctcaactatcaccttcccggtgggcaatattgcgccagaaggttcggtgatcaaatccaccgccattgacccctcgatgattgatgagcaaggtatctattaccataaaggtgtggcgaaggtttatctgtccgagaaaagtgcgatttacgatatcaaacatgacaagatcaaggcgggcgatattctggtcattattggcgttggaccttcaggtacagggatggaagaaacctaccaggttaccagtgccctgaagcatctgtcatacggtaagcatgtttcgttaatcaccgatgcacgtttctcgggcgtttctactggcgcgtgcatcggccatgtggggccagaagcgctggccggaggccccatcggtaaattacgcaccggggatttaattgaaattaaaattgattgtcgcgagcttcacggcgaagtcaatttcctcggaacccgtagcgatgaacaattaccttcacaggaggaggcaactgcaatattaaatgccagacccagccatcaggatttacttcccgatcctgaattgccagatgatacccggctatgggcaatgcttcaggccgtgagtggtgggacatggaccggttgtatttatgatgtaaacaaaattggcgcggctttgcgcgattttatgaataaaaactga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1602010_sequence/1
type: Sequence
persistent identity: BBa K1602010 sequence
display id: BBa_K1602010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccgcacaggttacctgcgtttgggatctgaaagcaaccctgggtgaaggtccgatttggcatggtgataccctgtggtttgttgatattaaacagcgcaaaatccacaactatcatccggcaacaggtgaacgttttagctttgatgcaccggatcaggtgacctttctggcaccgattgttggtgcaaccggctttgttgttggtctgaaaaccggcattcatcgttttcatcctgccaccggctttagcctgctgctggaagttgaagatgcagcactgaataatcgtccgaatgatgcaaccgttgatgcacagggtcgtctgtggttcggcaccatgcatgatggcgaagaaaataacagcggtagcctgtatcgtatggatctgacaggtgttgcacgcatggatcgtgatatttgtattaccaatggtccgtgtgttagtccggatggtaaaaccttttatcataccgataccctggaaaaaaccatctatgcatttgatctggcagaagatggtctgctgagcaataaacgtgtttttgttcagtttgcactgggtgatgatgtttacccggatggttcagttgttgatagcgaaggttatctgtggaccgcactgtggggtggttttggtgcagttcgttttagtccgcagggtgatgcagttacccgtattgaactgcctgcaccgaatgttaccaaaccgtgttttggtggtcctgatctgaaaacactgtattttaccaccgcacgtaaaggtctgagtgatgaaaccctggcacagtatccgctggcaggcggtgtttttgcagttccggttgatgttgcaggtcagccgcagcatgaagttcgtctggtttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1602011_sequence/1
type: Sequence
persistent identity: BBa K1602011 sequence
display id: BBa_K1602011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgccgcagtccgcgttgttcacgggaatcattccccctgtctccaccatttttaccgccgacggccagctcgataagccgggcaccgccgcgctgatcgacgatctgatcaaagcaggcgttgacggcctgttcttcctgggcagcggtggcgagttctcccagctcggcgccgaagagcgtaaagccattgcccgctttgctatcgatcatgtcgatcgtcgcgtgccggtgctgatcggcaccggcggcaccaacgcccgggaaaccatcgaactcagccagcacgcgcagcaggcgggcgcggacggcatcgtggtgatcaacccctactactggaaagtgtcggaagcgaacctgatccgctatttcgagcaggtggccgacagcgtcacgctgccggtgatgctctataacttcccggcgctgaccgggcaggatctgactccggcgctggtgaaaaccctcgccgactcgcgcagcaatattatcggcatcaaagacaccatcgactccgtcgcccacctgcgcagcatgatccataccgtcaaaggtgcccatccgcacttcaccgtgctctgcggctacgacgatcatctgttcaataccctgctgctcggcggcgacggggcgatatcggcgagcggcaactttgccccgcaggtgtcggtgaatcttctgaaagcctggcgcgacggggacgtggcgaaagcggccgggtatcatcagaccttgctgcaaattccgcagatgtatcagctggatacgccgtttgtgaacgtgattaaagaggcgatcgtgctctgcggtcgtcctgtctccacgcacgtgctgccgcccgcctcgccgctggacgagccgcgcaaggcgcagctgaaaaccctgctgcaacagctcaagctttgctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1602012_sequence/1
type: Sequence
persistent identity: BBa K1602012 sequence
display id: BBa_K1602012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtctgttcgcaatatttttgctgacgagagccacgatatttacaccgtcagaacgcacgccgatggcccggacggcgaactcccattaaccgcagagatgcttatcaaccgcccgagcggggatctgttcggtatgaccatgaatgccggaatgggttggtctccggacgagctggatcgggacggtattttactgctcagtacactcggtggcttacgcggcgcagacggtaaacccgtggcgctggcgttgcaccaggggcattacgaactggacatccagatgaaagcggcggccgaggttattaaagccaaccatgccctgccctatgccgtgtacgtctccgatccttgtgacgggcgtactcagggtacaacggggatgtttgattcgctaccataccgaaatgacgcatcgatggtaatgcgccgccttattcgctctctgcccgacgcgaaagcagttattggtgtggcgagttgcgataaggggcttccggccaccatgatggcactcgccgcgcagcacaacatcgcaaccgtgctggtccccggcggcgcgacgctgcccgcaaaggatggagaagacaacggcaaggtgcaaaccattggcgcacgcttcgccaatggcgaattatctctacaggacgcacgccgtgcgggctgtaaagcctgtgcctcttccggcggcggctgtcaatttttgggcactgccgggacatctcaggtggtggccgaaggattgggactggcaatcccacattcagccctggccccttccggtgagcctgtgtggcgggagatcgccagagcttccgcgcgagctgcgctgaacctgagtcaaaaaggcatcaccacccgggaaattctcaccgataaagcgatagagaatgcgatgacggtccatgccgcgttcggtggttcaacaaacctgctgttacacatcccggcaattgctcaccaggcaggttgccatatcccgaccgttgatgactggatccgcatcaacaagcgcgtgccccgactggtgagcgtactgcctaatggcccggtttatcatccaacggtcaatgcctttatggcaggtggtgtgccggaagtcatgttgcatctgcgcagcctcggattgttgcatgaagacgttatgacggttaccggcagcacgctgaaagaaaacctcgactggtgggagcactccgaacggcgtcagcggttcaagcaactcctgctcgatcaggaacaaatcaacgctgacgaagtgatcatgtctccgcagcaagcaaaagcgcgcggattaacctcaactatcaccttcccggtgggcaatattgcgccagaaggttcggtgatcaaatccaccgccattgacccctcgatgattgatgagcaaggtatctattaccataaaggtgtggcgaaggtttatctgtccgagaaaagtgcgatttacgatatcaaacatgacaagatcaaggcgggcgatattctggtcattattggcgttggaccttcaggtacagggatggaagaaacctaccaggttaccagtgccctgaagcatctgtcatacggtaagcatgtttcgttaatcaccgatgcacgtttctcgggcgtttctactggcgcgtgcatcggccatgtggggccagaagcgctggccggaggccccatcggtaaattacgcaccggggatttaattgaaattaaaattgattgtcgcgagcttcacggcgaagtcaatttcctcggaacccgtagcgatgaacaattaccttcacaggaggaggcaactgcaatattaaatgccagacccagccatcaggatttacttcccgatcctgaattgccagatgatacccggctatgggcaatgcttcaggccgtgagtggtgggacatggaccggttgtatttatgatgtaaacaaaattggcgcggctttgcgcgattttatgaataaaaactga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1602009_sequence/1
type: Sequence
persistent identity: BBa K1602009 sequence
display id: BBa_K1602009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcagcgccatttatccgagcctgaaaggtaaacgtgttgttattacaggtggtggtagcggtattggtgcaggtctgaccgcaggttttgcacgtcagggtgcagaagttatttttctggatattgcagatgaagatagccgtgcactggaagcagaactggcaggtagcccgattccgcctgtgtataaacgttgtgatctgatgaatctggaagccattaaagcagtgtttgccgaaattggtgatgttgatgttctggttaataacgcaggtaatgatgatcgtcataaactggcagatgttacaggtgcatattgggatgaacgcattaatgttaatctgcgccacatgctgttttgtacccaggcagttgcaccgggtatgaaaaaacgtggtggtggtgcagttattaactttggtagcattagctggcatctgggtttagaggatctggttctgtatgaaaccgcaaaagcaggtattgaaggtatgacccgtgccctggcacgtgaactgggtccggatgatattcgtgttacctgtgttgttccgggtaatgttaaaaccaaacgccaagagaaatggtatacaccggaaggtgaagcacagattgttgcagcacagtgtctgaaaggtcgtattgttccggaaaatgttgcagccctggtgctgtttctggcaagtgatgatgcaagcctgtgtacaggtcatgaatattggattgatgcaggttggcgttaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z