BBa_K1603000 1 BBa_K1603000 Fusion GPCR STE2MAM2 2015-09-17T11:00:00Z 2015-09-18T08:39:44Z The non-cytoplasmic N-terminal signal peptide from STE2 is a genomic sequence from Saccharomyces cerevisiae CEN.PK2 and the Pheromone P-factor receptor MAM2, without its signaling peptide, is a genomic sequence from Schizosaccharomyces pombe Δ8 h-. Fusion GPCR of the non-cytoplasmic N-terminal signal peptide from STE2 (Saccharomyces cerevisiae) and Pheromone P-factor receptor MAM2 (schizosaccharomyces pombe)without its signaling peptide. Allows S.cerevisiae to detect the Pheromone P-factor through the Pheromone pathway. false false _2020_ 27749 27749 9 false 20 base pair overlapping sequence between the signal peptide from STE2 and MAM2 had to be constructed through PCR to allow Gibson assembly of the two gene fragments. Added Prefix and Suffix through PCR. false John Hellgren annotation2471270 1 Signal peptide from STE2 range2471270 1 1 153 annotation2471271 1 MAM2 without signal peptide range2471271 1 154 1053 BBa_K1603000_sequence 1 atgtctgatgcggctccttcattgagcaatctattttatgatccaacgtataatcctggtcaaagcaccattaactacacttccatatatgggaatggatctaccatcactttcgatgagttgcaaggtttagttaacagtactgttactcagatgactttgtctgcccaacttgctttaggagtccttaccattttgatggtttgtctattgtcatcatccgaaaaacgaaaacacccagtttttgtttttaattcggcaagtattgttgcaatgtgtcttcgggccattttgaatatagtgaccatatgcagcaatagctacagtatcctggttaattacgggtttatcttaaacatggttcatatgtatgtccatgtgtttaatattttaattttgttgcttgcaccggtcatcatttttactgctgagatgagcatgatgattcaagttcgtataatttgtgcacatgatagaaagacacaaaggataatgactgttattagtgcctgcttaactgttttggttctcgcattttggattactaacatgtgtcaacagattcagtatctgttatggttaactccacttagcagcaagaccattgttggatactcttggccctactttattgctaaaatactttttgcttttagcattatttttcacagtggtgttttttcatacaaactctttcgtgccatattaatacggaaaaaaattgggcaatttccatttggtccgatgcagtgtattttagttattagctgccaatgtcttattgttccagctacctttactataatagatagttttatccatacgtatgatggctttagctctatgactcaatgtctgctaatcatttctcttcctctttcgagtttatgggcgtctagtacagctctgaaattgcaaagcatgaaaacttcatctgcgcaaggagaaaccaccgaggtttcgattagagttgatagaacgtttgatatcaaacatactcccagtgacgattattcgatttctgatgaatctgaaactaaaaagtggacgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z