BBa_K1603001 1 BBa_K1603001 pTEF1-pSUC2 2015-09-17T11:00:00Z 2015-09-18T05:57:31Z Genomic sequence from Saccharomyces cerevisiae CEN.PK2 The high expression pTEF1 promoter connected to pSUC2 promoter. Can be used for induced high expression of any coding part in Saccharomyces cerevisiae at low ATP levels. false false _2020_ 27749 27749 9 false Added 20 overlapping base pairs between the two gene fragments through PCR with primers with overhangs. This allowed the two promoters to be combined through Gibson assembly. Added Prefix and Suffix through PCR with primers with overhangs. false John Hellgren annotation2471278 1 pTEF1 range2471278 1 1 412 annotation2471279 1 pSUC2 range2471279 1 413 541 BBa_K1603001_sequence 1 atagcttcaaaatgtttctactccttttttactcttccagattttctcggactccgcgcatcgccgtaccacttcaaaacacccaagcacagcatactaaatttcccctctttcttcctctagggtgtcgttaattacccgtactaaaggtttggaaaagaaaaaagagaccgcctcgtttctttttcttcgtcgaaaaaggcaataaaaatttttatcacgtttctttttcttgaaaatttttttttttgatttttttctctttcgatgacctcccattgatatttaagttaataaacggtcttcaatttctcaagtttcagtttcatttttcttgttctattacaactttttttacttcttgctcattagaaagaaagcatagcaatctaatctaagttttaattacaaaataaatagatatgtattattcttcaaaacattctcttgttcttgtgctttttttttaccatatatcttacttttttttttctctcagagaaacaagcaaaacaaaaagcttttcttttcactaacgtat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z