LOCUS BBa_K1604020 5766 bp DNA linear 22-Dec-2024 DEFINITION araC-pBAD + beta-carotene ACCESSION BBa_K1604020 VERSION BBa_K1604020.1 FEATURES Location/Qualifiers promoter 1080..1203 /label=pBAD promoter 1043..1114 /label=PC CDS 1..1080 /label=araC promoter 1..1203 /label=BBa_K731201 RBS 1212..1215 /label=BBa_K118012 CDS 1222..2133 /label=BBa_I742151 misc_feature 2142..2144 /label=SacI RBS 2145..2151 /label=rbs RBS 2142..2151 /label=BBa_J15001 CDS 2158..3087 /label=crtB coding sequence CDS 2158..3090 /label=BBa_K118002 misc_feature 3099..3101 /label=SacI RBS 3102..3108 /label=rbs RBS 3099..3108 /label=BBa_J15001 misc_feature 3592..3597 /label=PstI site removed misc_feature 4003..4008 /label=PstI site removed CDS 3115..4593 /label=crtI coding sequence CDS 3115..4596 /label=BBa_K118003 RBS 4605..4608 /label=BBa_K118012 CDS 4615..5763 /label=crtY coding sequence CDS 4615..5766 /label=BBa_K118008 misc_feature 1212..5766 /label=BBa_K274200 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac gaaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac tcgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtagtg ctcaaaagca gcttcgcctg 181 actaatgcgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaacccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cgttaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccaccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgtactaga ggaggtacta gatgacggtc tgcgcaaaaa aacacgttca tctcactcgc 1261 gatgctgcgg agcagttact ggctgatatt gatcgacgcc ttgatcagtt attgcccgtg 1321 gagggagaac gggatgttgt gggtgccgcg atgcgtgaag gtgcgctggc accgggaaaa 1381 cgtattcgcc ccatgttgct gttgctgacc gcccgcgatc tgggttgcgc tgtcagccat 1441 gacggattac tggatttggc ctgtgcggtg gaaatggtcc acgcggcttc gctgatcctt 1501 gacgatatgc cctgcatgga cgatgcgaag ctgcggcgcg gacgccctac cattcattct 1561 cattacggag agcatgtggc aatactggcg gcggttgcct tgctgagtaa agcctttggc 1621 gtaattgccg atgcagatgg cctcacgccg ctggcaaaaa atcgggcggt ttctgaactg 1681 tcaaacgcca tcggcatgca aggattggtt cagggtcagt tcaaggatct gtctgaaggg 1741 gataagccgc gcagcgctga agctattttg atgacgaatc actttaaaac cagcacgctg 1801 ttttgtgcct ccatgcagat ggcctcgatt gttgcgaatg cctccagcga agcgcgtgat 1861 tgcctgcatc gtttttcact tgatcttggt caggcatttc aactgctgga cgatttgacc 1921 gatggcatga ccgacaccgg taaggatagc aatcaggacg ccggtaaatc gacgctggtc 1981 aatctgttag gcccgagggc ggttgaagaa cgtctgagac aacatcttca gcttgccagt 2041 gagcatctct ctgcggcctg ccaacacggg cacgccactc aacattttat tcaggcctgg 2101 tttgacaaaa aactcgctgc cgtcagttaa taatactaga gctcaaggag gtactagatg 2161 aataatccgt cgttactcaa tcatgcggtc gaaacgatgg cagttggctc gaaaagtttt 2221 gcgacagcct caaagttatt tgatgcaaaa acccggcgca gcgtactgat gctctacgcc 2281 tggtgccgcc attgtgacga tgttattgac gatcagacgc tgggctttca ggcccggcag 2341 cctgccttac aaacgcccga acaacgtctg atgcaacttg agatgaaaac gcgccaggcc 2401 tatgcaggat cgcagatgca cgaaccggcg tttgcggctt ttcaggaagt ggctatggct 2461 catgatatcg ccccggctta cgcgtttgat catctggaag gcttcgccat ggatgtacgc 2521 gaagcgcaat acagccaact ggatgatacg ctgcgctatt gctatcacgt tgcaggcgtt 2581 gtcggcttga tgatggcgca aatcatgggc gtgcgggata acgccacgct ggaccgcgcc 2641 tgtgaccttg ggctggcatt tcagttgacc aatattgctc gcgatattgt ggacgatgcg 2701 catgcgggcc gctgttatct gccggcaagc tggctggagc atgaaggtct gaacaaagag 2761 aattatgcgg cacctgaaaa ccgtcaggcg ctgagccgta tcgcccgtcg tttggtgcag 2821 gaagcagaac cttactattt gtctgccaca gccggcctgg cagggttgcc cctgcgttcc 2881 gcctgggcaa tcgctacggc gaagcaggtt taccggaaaa taggtgtcaa agttgaacag 2941 gccggtcagc aagcctggga tcagcggcag tcaacgacca cgcccgaaaa attaacgctg 3001 ctgctggccg cctctggtca ggcccttact tcccggatgc gggctcatcc tccccgccct 3061 gcgcatctct ggcagcgccc gctctaataa tactagagct caaggaggta ctagatgaaa 3121 ccaactacgg taattggtgc aggcttcggt ggcctggcac tggcaattcg tctacaagct 3181 gcggggatcc ccgtcttact gcttgaacaa cgtgataaac ccggcggtcg ggcttatgtc 3241 tacgaggatc aggggtttac ctttgatgca ggcccgacgg ttatcaccga tcccagtgcc 3301 attgaagaac tgtttgcact ggcaggaaaa cagttaaaag agtatgtcga actgctgccg 3361 gttacgccgt tttaccgcct gtgttgggag tcagggaagg tctttaatta cgataacgat 3421 caaacccggc tcgaagcgca gattcagcag tttaatcccc gcgatgtcga aggttatcgt 3481 cagtttctgg actattcacg cgcggtgttt aaagaaggct atctaaagct cggtactgtc 3541 ccttttttat cgttcagaga catgcttcgc gccgcacctc aactggcgaa actgcaagca 3601 tggagaagcg tttacagtaa ggttgccagt tacatcgaag atgaacatct gcgccaggcg 3661 ttttctttcc actcgctgtt ggtgggcggc aatcccttcg ccacctcatc catttatacg 3721 ttgatacacg cgctggagcg tgagtggggc gtctggtttc cgcgtggcgg caccggcgca 3781 ttagttcagg ggatgataaa gctgtttcag gatctgggtg gcgaagtcgt gttaaacgcc 3841 agagtcagcc atatggaaac gacaggaaac aagattgaag ccgtgcattt agaggacggt 3901 cgcaggttcc tgacgcaagc cgtcgcgtca aatgcagatg tggttcatac ctatcgcgac 3961 ctgttaagcc agcaccctgc cgcggttaag cagtccaaca aactgcaaac taagcgcatg 4021 agtaactctc tgtttgtgct ctattttggt ttgaatcacc atcatgatca gctcgcgcat 4081 cacacggttt gtttcggccc gcgttaccgc gagctgattg acgaaatttt taatcatgat 4141 ggcctcgcag aggacttctc actttatctg cacgcgccct gtgtcacgga ttcgtcactg 4201 gcgcctgaag gttgcggcag ttactatgtg ttggcgccgg tgccgcattt aggcaccgcg 4261 aacctcgact ggacggttga ggggccaaaa ctacgcgacc gtatttttgc gtaccttgag 4321 cagcattaca tgcctggctt acggagtcag ctggtcacgc accggatgtt tacgccgttt 4381 gattttcgcg accagcttaa tgcctatcat ggctcagcct tttctgtgga gcccgttctt 4441 acccagagcg cctggtttcg gccgcataac cgcgataaaa ccattactaa tctctacctg 4501 gtcggcgcag gcacgcatcc cggcgcaggc attcctggcg tcatcggctc ggcaaaagcg 4561 acagcaggtt tgatgctgga ggatctgata taataatact agaggaggta ctagatgcaa 4621 ccgcattatg atctgattct cgtgggggct ggactcgcga atggccttat cgccctgcgt 4681 cttcagcagc agcaacctga tatgcgtatt ttgcttatcg acgccgcacc ccaggcgggc 4741 gggaatcata cgtggtcatt tcaccacgat gatttgactg agagccaaca tcgttggata 4801 gctccgctgg tggttcatca ctggcccgac tatcaggtac gctttcccac acgccgtcgt 4861 aagctgaaca gcggctactt ttgtattact tctcagcgtt tcgctgaggt tttacagcga 4921 cagtttggcc cgcacttgtg gatggatacc gcggtcgcag aggttaatgc ggaatctgtt 4981 cggttgaaaa agggtcaggt tatcggtgcc cgcgcggtga ttgacgggcg gggttatgcg 5041 gcaaattcag cactgagcgt gggcttccag gcgtttattg gccaggaatg gcgattgagc 5101 cacccgcatg gtttatcgtc tcccattatc atggatgcca cggtcgatca gcaaaatggt 5161 tatcgcttcg tgtacagcct gccgctctcg ccgaccagat tgttaattga agacacgcac 5221 tatattgata atgcgacatt agatcctgaa tgcgcgcggc aaaatatttg cgactatgcc 5281 gcgcaacagg gttggcagct tcagacactg ctgcgagaag aacagggcgc cttacccatt 5341 actctgtcgg gcaatgccga cgcattctgg cagcagcgcc ccctggcctg tagtggatta 5401 cgtgccggtc tgttccatcc taccaccggc tattcactgc cgctggcggt tgccgtggcc 5461 gaccgcctga gtgcacttga tgtctttacg tcggcctcaa ttcaccatgc cattacgcat 5521 tttgcccgcg agcgctggca gcagcagggc tttttccgca tgctgaatcg catgctgttt 5581 ttagccggac ccgccgattc acgctggcgg gttatgcagc gtttttatgg tttacctgaa 5641 gatttaattg cccgttttta tgcgggaaaa ctcacgctga ccgatcggct acgtattctg 5701 agcggcaagc cgcctgttcc ggtattagca gcattgcaag ccattatgac gactcatcgt 5761 taataa //