BBa_K1604022
type: ComponentDefinition
persistent identity: BBa K1604022
display id: BBa_K1604022
version: 1
was generated by: iGEM to SBOL conversion
description: araC-pBAD + β-carotene + J23100 + blh
created: 2015-08-19T11:00:00Z
modified: 2015-09-17T05:19:54Z
mutable provenance: AraC-pBAD promoter comes from E.coli genome, while ctrEIBY are from Pantoea ananatis (formerly Erwinia uredovora) genome. Blh gene and its promoter are synthetized and codon optimezed by GeneScript.
owned by: james
owned by: myers
mutable description: This biobrick is obtained by the assembly of two parts K1604020 and K1604021. The composite part contains the five genes necessary for the retinal synthesis. The first part contains ctrEIBY under araC-pBAD, these four gene are responsive of beta-carotene production starting from FPP, a colurless precurse presents in E.coli. The second part contains blh under costitutive promoter. It encodes for the beta-carotene15-15'dioxygenase that catalizes the cleavege of a single molecule of beta-carotene into two molecules of retinal.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2021_
m user id: 11821
owner id: 24581
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The two parts was joined together by strandard assembly, before it beta-carotene was amplified by Phusion PCR.
star: false
creator: Veronica Pinamonti
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1604020
component: BBa_K1604021
sequence annotation: BBa_K1604021
sequence annotation: BBa_K1604020
sequence: https://synbiohub.org/public/igem/BBa_K1604022_sequence/1

BBa_K1604020
type: Component
persistent identity: component2449228
display id: component2449228
version: 1
top level: BBa_K1604022
definition: BBa_K1604020
access: public

BBa_K1604021
type: Component
persistent identity: component2449205
display id: component2449205
version: 1
top level: BBa_K1604022
definition: BBa_K1604021
access: public

BBa_K1604021
type: SequenceAnnotation
persistent identity: annotation2449205
display id: annotation2449205
version: 1
top level: BBa_K1604022
location: https://synbiohub.org/public/igem/BBa_K1604022/annotation2449205/range2449205/1
component: BBa_K1604021

https://synbiohub.org/public/igem/BBa_K1604022/annotation2449205/range2449205/1
type: Range
persistent identity: range2449205
display id: range2449205
version: 1
top level: BBa_K1604022
direction: direction/reverse
start: 1
end: 889
orientation: inline

BBa_K1604020
type: SequenceAnnotation
persistent identity: annotation2449228
display id: annotation2449228
version: 1
top level: BBa_K1604022
location: https://synbiohub.org/public/igem/BBa_K1604022/annotation2449228/range2449228/1
component: BBa_K1604020

https://synbiohub.org/public/igem/BBa_K1604022/annotation2449228/range2449228/1
type: Range
persistent identity: range2449228
display id: range2449228
version: 1
top level: BBa_K1604022
direction: direction/reverse
start: 898
end: 6663
orientation: inline

BBa_K1604020
type: ComponentDefinition
persistent identity: BBa K1604020
display id: BBa_K1604020
version: 1
was generated by: iGEM to SBOL conversion
description: araC-pBAD + beta-carotene
created: 2015-08-17T11:00:00Z
modified: 2015-09-11T09:15:32Z
owned by: james
owned by: myers
mutable description: This Composite Biobrick is created by standard assembly of Part BBa_K731201 and Part BBa_K274200, which are submitted by previous iGEM teams. The whole cassette is on plasmid pSB1C3 (high copy, Chloramphenicol resistance). BBa_K274200 contains the enzymes that convert colourless farnesyl pyrophosphate to orange beta-carotene. The carotene operon contains CrtE, CrtB, CrtI and CrtY each one with individual ribosome binding site. This Composite Biobrick has been put under inducible araC-pBAD promoter that is positively regulated by arabinose.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2021_
m user id: 11821
owner id: 24590
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Standard assembly between BBA_K731201 and a PCR amplification of BBa_K274200. BBa_K274200 was extracted by the 2012 distribution kit in pSB1A2 and it was amplified by PCR to restore a missing SpEI site.
star: false
creator: Elisa Godino
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K274200
component: BBa_K731201
sequence annotation: BBa_K731201
sequence annotation: BBa_K274200
sequence: https://synbiohub.org/public/igem/BBa_K1604020_sequence/1

BBa_K274200
type: Component
persistent identity: component2436253
display id: component2436253
version: 1
top level: BBa_K1604020
definition: BBa_K274200
access: public

BBa_K731201
type: Component
persistent identity: component2436235
display id: component2436235
version: 1
top level: BBa_K1604020
definition: BBa_K731201
access: public

BBa_K731201
type: SequenceAnnotation
persistent identity: annotation2436235
display id: annotation2436235
version: 1
top level: BBa_K1604020
location: https://synbiohub.org/public/igem/BBa_K1604020/annotation2436235/range2436235/1
component: BBa_K731201

https://synbiohub.org/public/igem/BBa_K1604020/annotation2436235/range2436235/1
type: Range
persistent identity: range2436235
display id: range2436235
version: 1
top level: BBa_K1604020
direction: direction/reverse
start: 1
end: 1203
orientation: inline

BBa_K274200
type: SequenceAnnotation
persistent identity: annotation2436253
display id: annotation2436253
version: 1
top level: BBa_K1604020
location: https://synbiohub.org/public/igem/BBa_K1604020/annotation2436253/range2436253/1
component: BBa_K274200

https://synbiohub.org/public/igem/BBa_K1604020/annotation2436253/range2436253/1
type: Range
persistent identity: range2436253
display id: range2436253
version: 1
top level: BBa_K1604020
direction: direction/reverse
start: 1212
end: 5766
orientation: inline

BBa_K1604021
type: ComponentDefinition
persistent identity: BBa K1604021
display id: BBa_K1604021
version: 1
was generated by: iGEM to SBOL conversion
description: J23100 + β-carotene 15,15’-dioxygenase (blh gene)
created: 2015-08-17T11:00:00Z
modified: 2015-09-18T03:56:40Z
mutable provenance: The sequence of uncultered cluster bacteria SAR86 was synthetized and codon optimized by GeneScript
owned by: james
owned by: myers
mutable description: blh encodes for β-carotene 15,15'-dioxygenase, the enzyme that cleaves one molecule of β-carotene into two molecules of retinal
discontinued: false
dominant: false
experience: experience/Works
group u list: _2021_
m user id: 24580
owner id: 24581
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The part was subcloned from pUC57 into pSB1C3
star: false
creator: Veronica Pinamonti
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1604019
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_K1604019
sequence: https://synbiohub.org/public/igem/BBa_K1604021_sequence/1

BBa_K1604019
type: Component
persistent identity: component2449242
display id: component2449242
version: 1
top level: BBa_K1604021
definition: BBa_K1604019
access: public

BBa_J23100
type: Component
persistent identity: component2449238
display id: component2449238
version: 1
top level: BBa_K1604021
definition: BBa_J23100
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2449238
display id: annotation2449238
version: 1
top level: BBa_K1604021
location: https://synbiohub.org/public/igem/BBa_K1604021/annotation2449238/range2449238/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1604021/annotation2449238/range2449238/1
type: Range
persistent identity: range2449238
display id: range2449238
version: 1
top level: BBa_K1604021
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K1604019
type: SequenceAnnotation
persistent identity: annotation2449242
display id: annotation2449242
version: 1
top level: BBa_K1604021
location: https://synbiohub.org/public/igem/BBa_K1604021/annotation2449242/range2449242/1
component: BBa_K1604019

https://synbiohub.org/public/igem/BBa_K1604021/annotation2449242/range2449242/1
type: Range
persistent identity: range2449242
display id: range2449242
version: 1
top level: BBa_K1604021
direction: direction/reverse
start: 44
end: 887
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

BBa_K731201
type: ComponentDefinition
persistent identity: BBa K731201
display id: BBa_K731201
version: 1
was generated by: iGEM to SBOL conversion
description: Arabinose inducible araC-pBAD promoter
created: 2012-08-02T11:00:00Z
modified: 2015-05-08T01:13:06Z
mutable provenance: E. coli genome
owned by: james
owned by: myers
mutable description: This E. coli promoter is positively regulated by arabinose. araC is the regulator protein, binding to arabinose prevents the repression of pBAD.
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 11997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: This part is similar to BBa_I0500, but it differs from that one in several point mutations in the sequence.
star: false
creator: Daniele Rossetto
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: araC
sequence annotation: PC
sequence annotation: pBAD
sequence: https://synbiohub.org/public/igem/BBa_K731201_sequence/1

araC
type: SequenceAnnotation
persistent identity: annotation2179313
display id: annotation2179313
version: 1
top level: BBa_K731201
location: https://synbiohub.org/public/igem/BBa_K731201/annotation2179313/range2179313/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K731201/annotation2179313/range2179313/1
type: Range
persistent identity: range2179313
display id: range2179313
version: 1
top level: BBa_K731201
direction: direction/reverse
start: 1
end: 1080
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation2179312
display id: annotation2179312
version: 1
top level: BBa_K731201
location: https://synbiohub.org/public/igem/BBa_K731201/annotation2179312/range2179312/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K731201/annotation2179312/range2179312/1
type: Range
persistent identity: range2179312
display id: range2179312
version: 1
top level: BBa_K731201
direction: direction/reverse
start: 1043
end: 1114
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2179311
display id: annotation2179311
version: 1
top level: BBa_K731201
location: https://synbiohub.org/public/igem/BBa_K731201/annotation2179311/range2179311/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K731201/annotation2179311/range2179311/1
type: Range
persistent identity: range2179311
display id: range2179311
version: 1
top level: BBa_K731201
direction: direction/forward
start: 1080
end: 1203
orientation: inline

BBa_K1604019
type: ComponentDefinition
persistent identity: BBa K1604019
display id: BBa_K1604019
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + β-carotene 15,15'-dioxygenase (blh gene)
created: 2015-09-10T11:00:00Z
modified: 2015-09-16T10:36:45Z
mutable provenance: The sequence of uncultered cluster bacteria SAR86 was synthetized and codon optimized by GeneScript
owned by: james
owned by: myers
mutable description: This part contains RBS and blh gene.
discontinued: false
dominant: false
experience: experience/None
group u list: _2021_
m user id: 24580
owner id: 24581
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Sequence was synthetized by GeneScript
star: false
creator: Veronica Pinamonti
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: RBS
sequence annotation: ATG
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1604019_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2449230
display id: annotation2449230
version: 1
top level: BBa_K1604019
location: https://synbiohub.org/public/igem/BBa_K1604019/annotation2449230/range2449230/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1604019/annotation2449230/range2449230/1
type: Range
persistent identity: range2449230
display id: range2449230
version: 1
top level: BBa_K1604019
direction: direction/forward
start: 1
end: 6
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2449231
display id: annotation2449231
version: 1
top level: BBa_K1604019
location: https://synbiohub.org/public/igem/BBa_K1604019/annotation2449231/range2449231/1
role: feature/start
role: SO:0000318

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type: Range
persistent identity: range2449231
display id: range2449231
version: 1
top level: BBa_K1604019
direction: direction/forward
start: 14
end: 16
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2449229
display id: annotation2449229
version: 1
top level: BBa_K1604019
location: https://synbiohub.org/public/igem/BBa_K1604019/annotation2449229/range2449229/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1604019/annotation2449229/range2449229/1
type: Range
persistent identity: range2449229
display id: range2449229
version: 1
top level: BBa_K1604019
direction: direction/forward
start: 839
end: 844
orientation: inline

BBa_K274200
type: ComponentDefinition
persistent identity: BBa K274200
display id: BBa_K274200
version: 1
was generated by: iGEM to SBOL conversion
description: CrtEBIY with rbs
created: 2009-09-17T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae. This Composite Biobrick is created by standard assembly of Part BBa_K118014, Part BBa_K118006, Part BBa_K118005 and Part BBa_K118013.
owned by: james
owned by: myers
mutable description: This Composite Biobrick is created by standard assembly of Part BBa_K118014 (CrtE with rbs), Part BBa_K118006 (CrtB with rbs), Part BBa_K118005 (CrtI with rbs) and Part BBa_K118013 (CrtY with rbs), which are submitted by previous iGEM teams. The whole cassette is on plasmid pSB1A2 (high copy, Ampicillin resistance). Together, enzymes CrtE, CrtB, CrtI and CrtY convert colourless farnesyl pyrophosphate to orange beta-carotene (via intermediates geranylgeranyl pyroiphosphate, phytoene and lycopene). The orange beta-carotene pigment can then be used as a coloured signal output, e.g. for biosensors. There is already individual ribosome binding site before each enzyme gene sequence. Internal restriction sites have been removed by previous iGEM teams. Please refer to Parts BBa_K118014, BBa_K118006, BBa_K118005 and BBa_K118013 for more information on individual Biobricks components. This Composite Biobrick has been put under constitutive promoter R0011 (see Part BBa_K274210) and arabinose-inducible promoter I0500 (see Part BBa_274220), transformed and tested in E.coli strain MG1655. Amount of beta-carotene produced can be measured by photospectrometer with absorbance at 455nm (beta-carotene extraction using acetone). A related Composite Biobrick is Part BBa_K274100 (CrtEBI with rbs), which "stops" at one step before beta-carotene, producing (and retaining) red lycopene pigment.
discontinued: false
dominant: true
experience: experience/Works
group u list: _374_
m user id: 0
owner id: 5416
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The colourless precursor, farnesyl pyrophosphate, is naturally produced in E.coli using Non-mevalonate Pathway (from pyruvate and glyceraldehyde-3-phosphate). Our experiences showed that this Biobrick works better in E.coli strains with up-regulated level of precursors. E.coli strain MG1655 demonostrates higher level of pigment (beta-carotene) production than strain TOP10.
star: false
creator: Siming Ma
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K118002
component: BBa_J15001
component: BBa_K118003
component: BBa_I742151
component: BBa_K118012
component: BBa_K118008
sequence annotation: BBa_K118012
sequence annotation: BBa_I742151
sequence annotation: BBa_J15001
sequence annotation: BBa_K118002
sequence annotation: BBa_K118003
sequence annotation: BBa_K118008
sequence: https://synbiohub.org/public/igem/BBa_K274200_sequence/1

BBa_K118002
type: Component
persistent identity: component2021586
display id: component2021586
version: 1
top level: BBa_K274200
definition: BBa_K118002
access: public

BBa_J15001
type: Component
persistent identity: component2021584
display id: component2021584
version: 1
top level: BBa_K274200
definition: BBa_J15001
access: public

BBa_K118003
type: Component
persistent identity: component2021593
display id: component2021593
version: 1
top level: BBa_K274200
definition: BBa_K118003
access: public

BBa_J15001
type: Component
persistent identity: component2021589
display id: component2021589
version: 1
top level: BBa_K274200
definition: BBa_J15001
access: public

BBa_I742151
type: Component
persistent identity: component2021581
display id: component2021581
version: 1
top level: BBa_K274200
definition: BBa_I742151
access: public

BBa_K118012
type: Component
persistent identity: component2021580
display id: component2021580
version: 1
top level: BBa_K274200
definition: BBa_K118012
access: public

BBa_K118008
type: Component
persistent identity: component2021596
display id: component2021596
version: 1
top level: BBa_K274200
definition: BBa_K118008
access: public

BBa_K118012
type: Component
persistent identity: component2021594
display id: component2021594
version: 1
top level: BBa_K274200
definition: BBa_K118012
access: public

BBa_K118012
type: SequenceAnnotation
persistent identity: annotation2021580
display id: annotation2021580
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021580/range2021580/1
component: BBa_K118012

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type: Range
persistent identity: range2021580
display id: range2021580
version: 1
top level: BBa_K274200
direction: direction/reverse
start: 1
end: 4
orientation: inline

BBa_I742151
type: SequenceAnnotation
persistent identity: annotation2021581
display id: annotation2021581
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021581/range2021581/1
component: BBa_I742151

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type: Range
persistent identity: range2021581
display id: range2021581
version: 1
top level: BBa_K274200
direction: direction/reverse
start: 11
end: 922
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2021584
display id: annotation2021584
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021584/range2021584/1
component: BBa_J15001

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type: Range
persistent identity: range2021584
display id: range2021584
version: 1
top level: BBa_K274200
direction: direction/reverse
start: 931
end: 940
orientation: inline

BBa_K118002
type: SequenceAnnotation
persistent identity: annotation2021586
display id: annotation2021586
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021586/range2021586/1
component: BBa_K118002

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type: Range
persistent identity: range2021586
display id: range2021586
version: 1
top level: BBa_K274200
direction: direction/reverse
start: 947
end: 1879
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2021589
display id: annotation2021589
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021589/range2021589/1
component: BBa_J15001

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type: Range
persistent identity: range2021589
display id: range2021589
version: 1
top level: BBa_K274200
direction: direction/reverse
start: 1888
end: 1897
orientation: inline

BBa_K118003
type: SequenceAnnotation
persistent identity: annotation2021593
display id: annotation2021593
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021593/range2021593/1
component: BBa_K118003

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type: Range
persistent identity: range2021593
display id: range2021593
version: 1
top level: BBa_K274200
direction: direction/reverse
start: 1904
end: 3385
orientation: inline

BBa_K118012
type: SequenceAnnotation
persistent identity: annotation2021594
display id: annotation2021594
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021594/range2021594/1
component: BBa_K118012

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type: Range
persistent identity: range2021594
display id: range2021594
version: 1
top level: BBa_K274200
direction: direction/reverse
start: 3394
end: 3397
orientation: inline

BBa_K118008
type: SequenceAnnotation
persistent identity: annotation2021596
display id: annotation2021596
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021596/range2021596/1
component: BBa_K118008

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type: Range
persistent identity: range2021596
display id: range2021596
version: 1
top level: BBa_K274200
direction: direction/reverse
start: 3404
end: 4555
orientation: inline

BBa_J15001
type: ComponentDefinition
persistent identity: BBa J15001
display id: BBa_J15001
version: 1
was generated by: iGEM to SBOL conversion
description: strong synthetic E. coli ribosome binding site with SacI site.
created: 2007-07-12T11:00:00Z
modified: 2015-08-31T04:08:32Z
mutable provenance: Synthetic.
owned by: james
owned by: myers
mutable description: This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector.
discontinued: false
dominant: false
experience: experience/None
group u list: _163_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest.
star: false
creator: Chris French
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: SacI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_J15001_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1938045
display id: annotation1938045
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
type: Range
persistent identity: range1938045
display id: range1938045
version: 1
top level: BBa_J15001
direction: direction/forward
start: 1
end: 3
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1938046
display id: annotation1938046
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
type: Range
persistent identity: range1938046
display id: range1938046
version: 1
top level: BBa_J15001
direction: direction/forward
start: 4
end: 10
orientation: inline

BBa_I742151
type: ComponentDefinition
persistent identity: BBa I742151
display id: BBa_I742151
version: 1
was generated by: iGEM to SBOL conversion
description: crtE (geranylgeranyl pyrophosphate synthase) coding sequence.
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
mutable description: crtE (geranylgeranyl pyrophosphate synthase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis parthway.
discontinued: false
dominant: false
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No important considerations.
star: false
creator: Chris French
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I742151_sequence/1

BBa_K118002
type: ComponentDefinition
persistent identity: BBa K118002
display id: BBa_K118002
version: 1
was generated by: iGEM to SBOL conversion
description: crtB coding sequence encoding phytoene synthase
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the coding sequence of crtB from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes phytoene synthase, part of the carotenoid biosynthesis pathway, which converts geranylgeranyl diphosphate to phytoene. Reference: Misawa,N., Nakagawa,M., Kobayashi,K., Yamano,S., Izawa,Y., Nakamura,K. and Harashima,K. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J. Bacteriol. 172 (12), 6704-6712 (1990)
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: No special considerations.
star: true
creator: Andrew Hall
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: crtB coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118002_sequence/1

crtB coding sequence
type: SequenceAnnotation
persistent identity: annotation1995701
display id: annotation1995701
version: 1
top level: BBa_K118002
location: https://synbiohub.org/public/igem/BBa_K118002/annotation1995701/range1995701/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K118002/annotation1995701/range1995701/1
type: Range
persistent identity: range1995701
display id: range1995701
version: 1
top level: BBa_K118002
direction: direction/forward
start: 1
end: 930
orientation: inline

BBa_K118003
type: ComponentDefinition
persistent identity: BBa K118003
display id: BBa_K118003
version: 1
was generated by: iGEM to SBOL conversion
description: crtI coding sequence encoding phytoene dehydrogenase
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the coding sequence of crtI from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes phytoene dehydrogenase, part of the carotenoid biosynthesis pathway, which converts Phytoene to Lycopene.
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The native crtI sequence contains two internal PstI sites. Primers crtImutf1 and crtImutf2 were ordered so that the MABEL protocol (invented for this purpose) could be used to remove these sites. The mutagenic primers were as follows: * crtImutf1: gtt tac agt aag gtt gcc * crtImutf2: agt aac tct ctg ttt gtg c In both cases, the PstI site CTGCAG was converted to CTGCAA, a silent mutation.
star: true
creator: Andrew Hall
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: crtI coding sequence
sequence annotation: PstI site removed
sequence: https://synbiohub.org/public/igem/BBa_K118003_sequence/1

crtI coding sequence
type: SequenceAnnotation
persistent identity: annotation1995702
display id: annotation1995702
version: 1
top level: BBa_K118003
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1995702/range1995702/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K118003/annotation1995702/range1995702/1
type: Range
persistent identity: range1995702
display id: range1995702
version: 1
top level: BBa_K118003
direction: direction/forward
start: 1
end: 1479
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation1968244
display id: annotation1968244
version: 1
top level: BBa_K118003
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1968244/range1968244/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K118003/annotation1968244/range1968244/1
type: Range
persistent identity: range1968244
display id: range1968244
version: 1
top level: BBa_K118003
direction: direction/forward
start: 478
end: 483
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation1968245
display id: annotation1968245
version: 1
top level: BBa_K118003
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1968245/range1968245/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K118003/annotation1968245/range1968245/1
type: Range
persistent identity: range1968245
display id: range1968245
version: 1
top level: BBa_K118003
direction: direction/forward
start: 889
end: 894
orientation: inline

BBa_K118012
type: ComponentDefinition
persistent identity: BBa K118012
display id: BBa_K118012
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic ribosome binding site added by Son of Babel procedure
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
mutable description: This is a 4bp synthetic ribosome binding site which can be added by fusion PCR using a reverse primer and the Son of Babel cloning system.
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The part can be added by fusion PCR using a Son of Babel reverse primer.
star: false
creator: Andrew Hall
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K118012_sequence/1

BBa_K118008
type: ComponentDefinition
persistent identity: BBa K118008
display id: BBa_K118008
version: 1
was generated by: iGEM to SBOL conversion
description: crtY coding sequence encoding lycopene B-cyclase
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the coding sequence of crtY from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes lycopene B-cyclase, part of the carotenoid biosynthesis pathway, which converts lycopene to B-carotene.
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: No special considerations
star: true
creator: Andrew Hall
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: crtY coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118008_sequence/1

crtY coding sequence
type: SequenceAnnotation
persistent identity: annotation1995716
display id: annotation1995716
version: 1
top level: BBa_K118008
location: https://synbiohub.org/public/igem/BBa_K118008/annotation1995716/range1995716/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K118008/annotation1995716/range1995716/1
type: Range
persistent identity: range1995716
display id: range1995716
version: 1
top level: BBa_K118008
direction: direction/forward
start: 1
end: 1149
orientation: inline

https://synbiohub.org/public/igem/BBa_K1604022_sequence/1
type: Sequence
persistent identity: BBa K1604022 sequence
display id: BBa_K1604022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agttgacggctagctcagtcctaggtacagtgctagctactagagaggaggaactactatgggtctgatgctgattgattggtgcgcactggctctggtggtttttattggtctgccgcatggtgccctggatgccgccattagcttctctatgatcagctctgccaaacgtattgcacgcctggctggtattctgctgatctatctgctgctggcgaccgcctttttcctgatctggtaccagctgccggcgttttcactgctgattttcctgctgatctcgattatccactttggcatggcagatttcaacgctagtccgtccaaactgaaatggccgcatattattgcacatggcggtgtcgtgaccgtgtggctgccgctgattcagaaaaacgaagttacgaaactgtttagcattctgaccaatggtccgacgccgatcctgtgggacattctgctgatctttttcctgtgctggagcattggcgtgtgtctgcatacctatgaaacgctgcgttctaaacactacaatatcgcatttgaactgattggcctgatcttcctggcctggtatgccccgccgctggtcacctttgctacgtacttttgcttcattcatagccgtcgccacttttctttcgtttggaaacagctgcaacatatgagtagcaagaaaatgatgattggtagcgcaattatcctgtcatgtacctcgtggctgattggcggtggcatctatttctttctgaacagcaaaatgatcgcctccgaagcagctctgcaaaccgtttttattggtctggccgcactgaccgtcccgcatatgattctgatcgattttattttccgtccgcactcatcgcgcattaaaatcaaaaattaataatactagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacgaaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctgactaatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccaccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaacccaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1604020_sequence/1
type: Sequence
persistent identity: BBa K1604020 sequence
display id: BBa_K1604020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacgaaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctgactaatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccaccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaacccaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgtactagaggaggtactagatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1604021_sequence/1
type: Sequence
persistent identity: BBa K1604021 sequence
display id: BBa_K1604021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaggaggaactactatgggtctgatgctgattgattggtgcgcactggctctggtggtttttattggtctgccgcatggtgccctggatgccgccattagcttctctatgatcagctctgccaaacgtattgcacgcctggctggtattctgctgatctatctgctgctggcgaccgcctttttcctgatctggtaccagctgccggcgttttcactgctgattttcctgctgatctcgattatccactttggcatggcagatttcaacgctagtccgtccaaactgaaatggccgcatattattgcacatggcggtgtcgtgaccgtgtggctgccgctgattcagaaaaacgaagttacgaaactgtttagcattctgaccaatggtccgacgccgatcctgtgggacattctgctgatctttttcctgtgctggagcattggcgtgtgtctgcatacctatgaaacgctgcgttctaaacactacaatatcgcatttgaactgattggcctgatcttcctggcctggtatgccccgccgctggtcacctttgctacgtacttttgcttcattcatagccgtcgccacttttctttcgtttggaaacagctgcaacatatgagtagcaagaaaatgatgattggtagcgcaattatcctgtcatgtacctcgtggctgattggcggtggcatctatttctttctgaacagcaaaatgatcgcctccgaagcagctctgcaaaccgtttttattggtctggccgcactgaccgtcccgcatatgattctgatcgattttattttccgtccgcactcatcgcgcattaaaatcaaaaattaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K731201_sequence/1
type: Sequence
persistent identity: BBa K731201 sequence
display id: BBa_K731201_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacgaaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctgactaatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccaccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaacccaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1604019_sequence/1
type: Sequence
persistent identity: BBa K1604019 sequence
display id: BBa_K1604019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggaggaactactatgggtctgatgctgattgattggtgcgcactggctctggtggtttttattggtctgccgcatggtgccctggatgccgccattagcttctctatgatcagctctgccaaacgtattgcacgcctggctggtattctgctgatctatctgctgctggcgaccgcctttttcctgatctggtaccagctgccggcgttttcactgctgattttcctgctgatctcgattatccactttggcatggcagatttcaacgctagtccgtccaaactgaaatggccgcatattattgcacatggcggtgtcgtgaccgtgtggctgccgctgattcagaaaaacgaagttacgaaactgtttagcattctgaccaatggtccgacgccgatcctgtgggacattctgctgatctttttcctgtgctggagcattggcgtgtgtctgcatacctatgaaacgctgcgttctaaacactacaatatcgcatttgaactgattggcctgatcttcctggcctggtatgccccgccgctggtcacctttgctacgtacttttgcttcattcatagccgtcgccacttttctttcgtttggaaacagctgcaacatatgagtagcaagaaaatgatgattggtagcgcaattatcctgtcatgtacctcgtggctgattggcggtggcatctatttctttctgaacagcaaaatgatcgcctccgaagcagctctgcaaaccgtttttattggtctggccgcactgaccgtcccgcatatgattctgatcgattttattttccgtccgcactcatcgcgcattaaaatcaaaaattaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K274200_sequence/1
type: Sequence
persistent identity: BBa K274200 sequence
display id: BBa_K274200_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaggtactagatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataatactagagctcaaggaggtactagatgaataatccgtcgttactcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaataatactagagctcaaggaggtactagatgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J15001_sequence/1
type: Sequence
persistent identity: BBa J15001 sequence
display id: BBa_J15001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcaaggagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742151_sequence/1
type: Sequence
persistent identity: BBa I742151 sequence
display id: BBa_I742151_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118002_sequence/1
type: Sequence
persistent identity: BBa K118002 sequence
display id: BBa_K118002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaataatccgtcgttactcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118003_sequence/1
type: Sequence
persistent identity: BBa K118003 sequence
display id: BBa_K118003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactgcaagcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagcagtccaacaaactgcaaactaagcgcatgagtaactctctgtttgtgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaaatttttaatcatgatggcctcgcagaggacttctcactttatctgcacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcagttactatgtgttggcgccggtgccgcatttaggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgaccgtatttttgcgtaccttgagcagcattacatgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgtttgattttcgcgaccagcttaatgcctatcatggctcagccttttctgtggagcccgttcttacccagagcgcctggtttcggccgcataaccgcgataaaaccattactaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcatcggctcggcaaaagcgacagcaggtttgatgctggaggatctgatataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118012_sequence/1
type: Sequence
persistent identity: BBa K118012 sequence
display id: BBa_K118012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: gagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118008_sequence/1
type: Sequence
persistent identity: BBa K118008 sequence
display id: BBa_K118008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcaaccgcattatgatctgattctcgtgggggctggactcgcgaatggccttatcgccctgcgtcttcagcagcagcaacctgatatgcgtattttgcttatcgacgccgcaccccaggcgggcgggaatcatacgtggtcatttcaccacgatgatttgactgagagccaacatcgttggatagctccgctggtggttcatcactggcccgactatcaggtacgctttcccacacgccgtcgtaagctgaacagcggctacttttgtattacttctcagcgtttcgctgaggttttacagcgacagtttggcccgcacttgtggatggataccgcggtcgcagaggttaatgcggaatctgttcggttgaaaaagggtcaggttatcggtgcccgcgcggtgattgacgggcggggttatgcggcaaattcagcactgagcgtgggcttccaggcgtttattggccaggaatggcgattgagccacccgcatggtttatcgtctcccattatcatggatgccacggtcgatcagcaaaatggttatcgcttcgtgtacagcctgccgctctcgccgaccagattgttaattgaagacacgcactatattgataatgcgacattagatcctgaatgcgcgcggcaaaatatttgcgactatgccgcgcaacagggttggcagcttcagacactgctgcgagaagaacagggcgccttacccattactctgtcgggcaatgccgacgcattctggcagcagcgccccctggcctgtagtggattacgtgccggtctgttccatcctaccaccggctattcactgccgctggcggttgccgtggccgaccgcctgagtgcacttgatgtctttacgtcggcctcaattcaccatgccattacgcattttgcccgcgagcgctggcagcagcagggctttttccgcatgctgaatcgcatgctgtttttagccggacccgccgattcacgctggcgggttatgcagcgtttttatggtttacctgaagatttaattgcccgtttttatgcgggaaaactcacgctgaccgatcggctacgtattctgagcggcaagccgcctgttccggtattagcagcattgcaagccattatgacgactcatcgttaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z