LOCUS BBa_K1604025 8673 bp DNA linear 22-Nov-2024 DEFINITION araC-pBAD + proteorhodopsin + j23100 + blh + araC-pBAD + β-Carotene ACCESSION BBa_K1604025 VERSION BBa_K1604025.1 FEATURES Location/Qualifiers promoter 1080..1203 /label=pBAD promoter 1043..1114 /label=PC CDS 1..1080 /label=araC promoter 1..1203 /label=BBa_K731201 RBS 1212..1217 /label=strong RBS misc_feature 1226..1228 /label=ATG CDS 1226..2002 /label=Proteorhodopsin misc_feature 1996..2002 /label=2 x stop misc_feature 1212..2002 /label=BBa_K1604000 misc_feature 1..2002 /label=BBa_K1604010 promoter 2011..2045 /label=BBa_J23100 misc_feature 2892..2897 /label=stop RBS 2054..2059 /label=RBS misc_feature 2067..2069 /label=ATG misc_feature 2054..2897 /label=BBa_K1604019 misc_feature 2011..2899 /label=BBa_K1604021 promoter 3987..4110 /label=pBAD promoter 3950..4021 /label=PC CDS 2908..3987 /label=araC promoter 2908..4110 /label=BBa_K731201 RBS 4119..4122 /label=BBa_K118012 CDS 4129..5040 /label=BBa_I742151 misc_feature 5049..5051 /label=SacI RBS 5052..5058 /label=rbs RBS 5049..5058 /label=BBa_J15001 CDS 5065..5994 /label=crtB coding sequence CDS 5065..5997 /label=BBa_K118002 misc_feature 6006..6008 /label=SacI RBS 6009..6015 /label=rbs RBS 6006..6015 /label=BBa_J15001 misc_feature 6499..6504 /label=PstI site removed misc_feature 6910..6915 /label=PstI site removed CDS 6022..7500 /label=crtI coding sequence CDS 6022..7503 /label=BBa_K118003 RBS 7512..7515 /label=BBa_K118012 CDS 7522..8670 /label=crtY coding sequence CDS 7522..8673 /label=BBa_K118008 misc_feature 4119..8673 /label=BBa_K274200 misc_feature 2908..8673 /label=BBa_K1604020 misc_feature 2011..8673 /label=BBa_K1604022 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac gaaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac tcgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtagtg ctcaaaagca gcttcgcctg 181 actaatgcgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaacccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cgttaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccaccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgtactaga gaggaggtac tagagatgtc tatcatcaaa tccctgaaat ctgttgctat 1261 tgcgtctctc gcgattctca tccctagcat cgccctggca gcaggtggta atctcgagcc 1321 gaacgaccca gttggtatca ccttttggct gatttccatc gctatggtcg cggccacggt 1381 attcttcctg atggagtctc tccgtgtaga cggtaaatgg cgtacgtcta tgatcgttgg 1441 cggtctggtg actctggttg cggctgttca ctacttctac atgcgtgacg tctgggttgc 1501 taccggtgct tctcctaccg tattccgtta cgtcgattgg ctcattaccg ttccgctcca 1561 gatgattgag ttttacctca tcctggcggc gtgcaccgca atcgccgtgg gtgttttctg 1621 gcgtctgatg atcggcacga tggttatgct gattggtggt tacctgggcg aggctggttt 1681 catcaacgct actgtgggtt ttgtcatcgg catggcgggc tggggctaca tcctgtacga 1741 gatctttgcg ggtgaagcag gcaaagtcgc agccgagggt gctcctcctt ctgttcagtc 1801 tgcgtttaat accatgcgcc tcattgttac cattggttgg gcgatctacc cgctgggtta 1861 cttcttcggt tacatgaccg gtggtgttga cgcgaactct ctgaacctga tctacaacgt 1921 agcggatgtg gttaacaaga tcggtttctg cctggctatt tgggcagcgg ctacgtctca 1981 atctgaagcg gcgaaatgat agtactagag agttgacggc tagctcagtc ctaggtacag 2041 tgctagctac tagagaggag gaactactat gggtctgatg ctgattgatt ggtgcgcact 2101 ggctctggtg gtttttattg gtctgccgca tggtgccctg gatgccgcca ttagcttctc 2161 tatgatcagc tctgccaaac gtattgcacg cctggctggt attctgctga tctatctgct 2221 gctggcgacc gcctttttcc tgatctggta ccagctgccg gcgttttcac tgctgatttt 2281 cctgctgatc tcgattatcc actttggcat ggcagatttc aacgctagtc cgtccaaact 2341 gaaatggccg catattattg cacatggcgg tgtcgtgacc gtgtggctgc cgctgattca 2401 gaaaaacgaa gttacgaaac tgtttagcat tctgaccaat ggtccgacgc cgatcctgtg 2461 ggacattctg ctgatctttt tcctgtgctg gagcattggc gtgtgtctgc atacctatga 2521 aacgctgcgt tctaaacact acaatatcgc atttgaactg attggcctga tcttcctggc 2581 ctggtatgcc ccgccgctgg tcacctttgc tacgtacttt tgcttcattc atagccgtcg 2641 ccacttttct ttcgtttgga aacagctgca acatatgagt agcaagaaaa tgatgattgg 2701 tagcgcaatt atcctgtcat gtacctcgtg gctgattggc ggtggcatct atttctttct 2761 gaacagcaaa atgatcgcct ccgaagcagc tctgcaaacc gtttttattg gtctggccgc 2821 actgaccgtc ccgcatatga ttctgatcga ttttattttc cgtccgcact catcgcgcat 2881 taaaatcaaa aattaataat actagagtta tgacaacttg acggctacat cattcacttt 2941 ttcttcacaa ccggcacgaa actcgctcgg gctggccccg gtgcattttt taaatactcg 3001 cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg 3061 ggtagtgctc aaaagcagct tcgcctgact aatgcgttgg tcctcgcgcc agcttaagac 3121 gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg 3181 ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca 3241 agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg 3301 ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc 3361 ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc 3421 cgggcgaaag aaacccgtat tggcaaatat tgacggccag ttaagccatt catgccagta 3481 ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc 3541 gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc agacaaattc 3601 tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt 3661 cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa 3721 acccgccacc agatgggcgt taaacgagta tcccggcagc aggggatcat tttgcgcttc 3781 agccatactt ttcatactcc caccattcag agaagaaacc aattgtccat attgcatcag 3841 acattgccgt cactgcgtct tttactggct cttctcgcta acccaaccgg taaccccgct 3901 tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag 3961 tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta 4021 tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact 4081 ctctactgtt tctccatacc cgtttttttg tactagagga ggtactagat gacggtctgc 4141 gcaaaaaaac acgttcatct cactcgcgat gctgcggagc agttactggc tgatattgat 4201 cgacgccttg atcagttatt gcccgtggag ggagaacggg atgttgtggg tgccgcgatg 4261 cgtgaaggtg cgctggcacc gggaaaacgt attcgcccca tgttgctgtt gctgaccgcc 4321 cgcgatctgg gttgcgctgt cagccatgac ggattactgg atttggcctg tgcggtggaa 4381 atggtccacg cggcttcgct gatccttgac gatatgccct gcatggacga tgcgaagctg 4441 cggcgcggac gccctaccat tcattctcat tacggagagc atgtggcaat actggcggcg 4501 gttgccttgc tgagtaaagc ctttggcgta attgccgatg cagatggcct cacgccgctg 4561 gcaaaaaatc gggcggtttc tgaactgtca aacgccatcg gcatgcaagg attggttcag 4621 ggtcagttca aggatctgtc tgaaggggat aagccgcgca gcgctgaagc tattttgatg 4681 acgaatcact ttaaaaccag cacgctgttt tgtgcctcca tgcagatggc ctcgattgtt 4741 gcgaatgcct ccagcgaagc gcgtgattgc ctgcatcgtt tttcacttga tcttggtcag 4801 gcatttcaac tgctggacga tttgaccgat ggcatgaccg acaccggtaa ggatagcaat 4861 caggacgccg gtaaatcgac gctggtcaat ctgttaggcc cgagggcggt tgaagaacgt 4921 ctgagacaac atcttcagct tgccagtgag catctctctg cggcctgcca acacgggcac 4981 gccactcaac attttattca ggcctggttt gacaaaaaac tcgctgccgt cagttaataa 5041 tactagagct caaggaggta ctagatgaat aatccgtcgt tactcaatca tgcggtcgaa 5101 acgatggcag ttggctcgaa aagttttgcg acagcctcaa agttatttga tgcaaaaacc 5161 cggcgcagcg tactgatgct ctacgcctgg tgccgccatt gtgacgatgt tattgacgat 5221 cagacgctgg gctttcaggc ccggcagcct gccttacaaa cgcccgaaca acgtctgatg 5281 caacttgaga tgaaaacgcg ccaggcctat gcaggatcgc agatgcacga accggcgttt 5341 gcggcttttc aggaagtggc tatggctcat gatatcgccc cggcttacgc gtttgatcat 5401 ctggaaggct tcgccatgga tgtacgcgaa gcgcaataca gccaactgga tgatacgctg 5461 cgctattgct atcacgttgc aggcgttgtc ggcttgatga tggcgcaaat catgggcgtg 5521 cgggataacg ccacgctgga ccgcgcctgt gaccttgggc tggcatttca gttgaccaat 5581 attgctcgcg atattgtgga cgatgcgcat gcgggccgct gttatctgcc ggcaagctgg 5641 ctggagcatg aaggtctgaa caaagagaat tatgcggcac ctgaaaaccg tcaggcgctg 5701 agccgtatcg cccgtcgttt ggtgcaggaa gcagaacctt actatttgtc tgccacagcc 5761 ggcctggcag ggttgcccct gcgttccgcc tgggcaatcg ctacggcgaa gcaggtttac 5821 cggaaaatag gtgtcaaagt tgaacaggcc ggtcagcaag cctgggatca gcggcagtca 5881 acgaccacgc ccgaaaaatt aacgctgctg ctggccgcct ctggtcaggc ccttacttcc 5941 cggatgcggg ctcatcctcc ccgccctgcg catctctggc agcgcccgct ctaataatac 6001 tagagctcaa ggaggtacta gatgaaacca actacggtaa ttggtgcagg cttcggtggc 6061 ctggcactgg caattcgtct acaagctgcg gggatccccg tcttactgct tgaacaacgt 6121 gataaacccg gcggtcgggc ttatgtctac gaggatcagg ggtttacctt tgatgcaggc 6181 ccgacggtta tcaccgatcc cagtgccatt gaagaactgt ttgcactggc aggaaaacag 6241 ttaaaagagt atgtcgaact gctgccggtt acgccgtttt accgcctgtg ttgggagtca 6301 gggaaggtct ttaattacga taacgatcaa acccggctcg aagcgcagat tcagcagttt 6361 aatccccgcg atgtcgaagg ttatcgtcag tttctggact attcacgcgc ggtgtttaaa 6421 gaaggctatc taaagctcgg tactgtccct tttttatcgt tcagagacat gcttcgcgcc 6481 gcacctcaac tggcgaaact gcaagcatgg agaagcgttt acagtaaggt tgccagttac 6541 atcgaagatg aacatctgcg ccaggcgttt tctttccact cgctgttggt gggcggcaat 6601 cccttcgcca cctcatccat ttatacgttg atacacgcgc tggagcgtga gtggggcgtc 6661 tggtttccgc gtggcggcac cggcgcatta gttcagggga tgataaagct gtttcaggat 6721 ctgggtggcg aagtcgtgtt aaacgccaga gtcagccata tggaaacgac aggaaacaag 6781 attgaagccg tgcatttaga ggacggtcgc aggttcctga cgcaagccgt cgcgtcaaat 6841 gcagatgtgg ttcataccta tcgcgacctg ttaagccagc accctgccgc ggttaagcag 6901 tccaacaaac tgcaaactaa gcgcatgagt aactctctgt ttgtgctcta ttttggtttg 6961 aatcaccatc atgatcagct cgcgcatcac acggtttgtt tcggcccgcg ttaccgcgag 7021 ctgattgacg aaatttttaa tcatgatggc ctcgcagagg acttctcact ttatctgcac 7081 gcgccctgtg tcacggattc gtcactggcg cctgaaggtt gcggcagtta ctatgtgttg 7141 gcgccggtgc cgcatttagg caccgcgaac ctcgactgga cggttgaggg gccaaaacta 7201 cgcgaccgta tttttgcgta ccttgagcag cattacatgc ctggcttacg gagtcagctg 7261 gtcacgcacc ggatgtttac gccgtttgat tttcgcgacc agcttaatgc ctatcatggc 7321 tcagcctttt ctgtggagcc cgttcttacc cagagcgcct ggtttcggcc gcataaccgc 7381 gataaaacca ttactaatct ctacctggtc ggcgcaggca cgcatcccgg cgcaggcatt 7441 cctggcgtca tcggctcggc aaaagcgaca gcaggtttga tgctggagga tctgatataa 7501 taatactaga ggaggtacta gatgcaaccg cattatgatc tgattctcgt gggggctgga 7561 ctcgcgaatg gccttatcgc cctgcgtctt cagcagcagc aacctgatat gcgtattttg 7621 cttatcgacg ccgcacccca ggcgggcggg aatcatacgt ggtcatttca ccacgatgat 7681 ttgactgaga gccaacatcg ttggatagct ccgctggtgg ttcatcactg gcccgactat 7741 caggtacgct ttcccacacg ccgtcgtaag ctgaacagcg gctacttttg tattacttct 7801 cagcgtttcg ctgaggtttt acagcgacag tttggcccgc acttgtggat ggataccgcg 7861 gtcgcagagg ttaatgcgga atctgttcgg ttgaaaaagg gtcaggttat cggtgcccgc 7921 gcggtgattg acgggcgggg ttatgcggca aattcagcac tgagcgtggg cttccaggcg 7981 tttattggcc aggaatggcg attgagccac ccgcatggtt tatcgtctcc cattatcatg 8041 gatgccacgg tcgatcagca aaatggttat cgcttcgtgt acagcctgcc gctctcgccg 8101 accagattgt taattgaaga cacgcactat attgataatg cgacattaga tcctgaatgc 8161 gcgcggcaaa atatttgcga ctatgccgcg caacagggtt ggcagcttca gacactgctg 8221 cgagaagaac agggcgcctt acccattact ctgtcgggca atgccgacgc attctggcag 8281 cagcgccccc tggcctgtag tggattacgt gccggtctgt tccatcctac caccggctat 8341 tcactgccgc tggcggttgc cgtggccgac cgcctgagtg cacttgatgt ctttacgtcg 8401 gcctcaattc accatgccat tacgcatttt gcccgcgagc gctggcagca gcagggcttt 8461 ttccgcatgc tgaatcgcat gctgttttta gccggacccg ccgattcacg ctggcgggtt 8521 atgcagcgtt tttatggttt acctgaagat ttaattgccc gtttttatgc gggaaaactc 8581 acgctgaccg atcggctacg tattctgagc ggcaagccgc ctgttccggt attagcagca 8641 ttgcaagcca ttatgacgac tcatcgttaa taa //