LOCUS       BBa_K1605003             926 bp    DNA     linear       27-Dec-2024
DEFINITION  Ptet mRFP nifZ RBS
ACCESSION   BBa_K1605003
VERSION     BBa_K1605003.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=BBa_K1605002
     CDS             84..758
                     /label=mrfp1
     misc_feature    765..789
                     /label=Help:Barcodes
     CDS             84..789
                     /label=BBa_E1010
     stem_loop       809..852
                     /label=stem_loop
     misc_feature    798..877
                     /label=BBa_B0010
     terminator      798..877
                     /label=BBa_B0010
     stem_loop       893..912
                     /label=T7 TE
     misc_feature    919..919
                     /label=stop
     polyA_site      913..926
                     /label=polya
     misc_feature    886..926
                     /label=BBa_B0012
     terminator      886..926
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agagaggaag gtctaaatac tagatggctt cctccgaaga cgttatcaaa gagttcatgc
      121 gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc gaaggtgaag
      181 gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc aaaggtggtc
      241 cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc aaagcttacg
      301 ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa ggtttcaaat
      361 gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag gactcctccc
      421 tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc ccgtccgacg
      481 gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt atgtacccgg
      541 aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact
      601 acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt
      661 acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc atcgttgaac
      721 agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga tagtgctagt
      781 gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      841 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
      901 ttcgggtggg cctttctgcg tttata
//