##gff-version 3
##sequence-region BBa_K1605019 1 927
BBa_K1605019	.	terminator	799	878	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K1605019	.	engineered_region	799	878	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1605019	.	stem_loop	810	853	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1605019	.	CDS	85	790	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K1605019	.	engineered_tag	766	790	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1605019	.	CDS	85	759	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1605019	.	terminator	887	927	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K1605019	.	engineered_region	887	927	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1605019	.	stop_codon	920	920	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1605019	.	stem_loop	894	913	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1605019	.	polyA_site	914	927	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1605019	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1605019	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1605019	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1605019	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1605019	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1605019	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1605019	.	ribosome_entry_site	63	78	.	+	0	ID=BBa_K1605018;Name=BBa_K1605018
>BBa_K1605019
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagttaaaggatttaaccct
actagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtca
cgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggt
ggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctg
acatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgt
tgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccg
tccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctc
tgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggc
taaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacacc
atcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
tactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtg
aacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata