BBa_K590087 1 KumaMax KumaMax: Kumamolisin-As_N291D, G319S, D358G, D368H 2011-09-21T11:00:00Z 2015-05-08T01:12:48Z Engineered but the original protein is form Alicyclobacillus sendaiensis A mutated Kumamolisin-As enzyme aimed to combat gluten intolerance by increased activity with the PQLP peptide, an antigenic epitope in gliadin. This mutant is the best combinatorial mutant found and has point mutations at residues 291, 319, 358, and 368 from Asparagine to Aspartate, Glycine to Serine, Aspartate to Glycine, and Aspartate to Histidine, respectively. false false _761_ 0 5005 9 It's complicated true Aimed to increase binding affinity to the immunogenic PQLP peptide found in gluten false Sydney Gordon, Daniel Hadidi, Elizabeth Stanley, Sarah Wolf, Angus Toland, Sean Wu BBa_K1606001 1 BBa_K1606001 Kumamolisin in alpha secretion factor 2015-08-27T11:00:00Z 2015-08-28T07:56:00Z Original protein comes form Alicyclobacillus sendaiensis. (Genetically engineered.) Taking kumamolisin and putting it in a vector of yeast that has alpha secretion factor then putting the plasmid in yeast. The yeast will produce and secrete kumamolisin enzyme to its external environment and will destroys gluten while the bread continues to be fermented. false false _2023_ 16929 16929 9 true "Point mutations at residues 291, 319, 358, and 368 from Asparagine to Aspartate, Glycine to Serine, Aspartate to Glycine, and Aspartate to Histidine, respectively." and adding it into alpha secretion factor. false Ilkem Kumru component2439367 1 BBa_K590087 annotation2439367 1 BBa_K590087 range2439367 1 1 1701 BBa_K590087_sequence 1 atgagtgatatggaaaaaccgtggaaagaaggtgaagaagcccgcgcagtgctgcaaggtcatgctcgtgcgcaggcaccgcaagcagtcgataaaggcccggtggcaggtgacgaacgcatggctgttaccgtggttctgcgtcgccagcgtgcaggtgaactggcggcccacgtggaacgtcaagcagctattgctccgcatgcgcgcgaacacctgaaacgtgaagcgtttgcggccagtcatggtgcgtccctggatgactttgccgaactgcgtcgcttcgcagatgctcacggcctggcgctggaccgtgcaaacgttgcagctggcaccgccgttctgtctggtccggtcgatgcaatcaatcgcgcttttggtgtggaactgcgtcatttcgatcacccggacggctcatatcgttcgtacctgggtgaagtcaccgtgccggccagtattgcaccgatgatcgaagcggttctgggcctggatacgcgtccggtcgcccgcccgcattttcgtatgcagcgtcgcgcagaaggcggtttcgaagctcgttcccaagcggcggcaccgaccgcatatacgccgctggatgttgcgcaggcctaccaatttccggaaggtctggacggccagggtcaatgcattgccattatcgaactgggcggtggctatgatgaagcttcactggcgcagtacttcgcgtcgctgggcgtgccggcaccgcaagtcgtgagtgtttccgtcgatggtgcgagcaaccagccgaccggtgatccgtctggtccggacggtgaagtggaactggatatcgaagttgcaggcgctctggcgccgggtgccaaatttgcagtgtatttcgcgccggataccgatgccggttttctggacgcgattaccacggccatccacgatccgacgctgaaaccgagcgttgtctcaatttcgtggagcggcccggaagacagctggacctctgctgcgatcgccgcaatgaaccgtgcgtttctggatgctgcggccctgggtgtgaccgttctggcagctgcgggcgacagcggttctacgggtggcgaacaggacggtctgtatcatgtgcatttcccggccgcatcaccgtacgttctggcgtgcggtggcacgcgcctggtcgcatcgggtggccgtattgcgcaggaaaccgtctggaacgatggtccggacggtggtgcaacgggtggcggtgtgagccgcatcttcccgctgccggcatggcaggaacacgctaacgttccgccgtctgcaaatccgggcgcgagcagcggccgtggtgtcccggatctggctggtaatgcggacccggcaaccggttatgaagtggttattgatggcgaagcaaccgtcatcggcggtacgagcgccgtggcaccgctgtttgctgcgctggttgcgcgtattaaccagaaactgggcaaagcagttggttatctgaatccgaccctgtaccaactgccggcagatgttttccatgacatcacggagggtaacaatgatattgcaaaccgtgcgcagatttatcaagcaggtccgggctgggacccgtgtaccggtctgggttcaccgattggtgtgcgtctgctgcaagcactgctgccgagtgcctcccagccgcaaccgggtagtacggaaaatctgtactttcagtccggcgcgctcgag BBa_K1606001_sequence 1 atgagtgatatggaaaaaccgtggaaagaaggtgaagaagcccgcgcagtgctgcaaggtcatgctcgtgcgcaggcaccgcaagcagtcgataaaggcccggtggcaggtgacgaacgcatggctgttaccgtggttctgcgtcgccagcgtgcaggtgaactggcggcccacgtggaacgtcaagcagctattgctccgcatgcgcgcgaacacctgaaacgtgaagcgtttgcggccagtcatggtgcgtccctggatgactttgccgaactgcgtcgcttcgcagatgctcacggcctggcgctggaccgtgcaaacgttgcagctggcaccgccgttctgtctggtccggtcgatgcaatcaatcgcgcttttggtgtggaactgcgtcatttcgatcacccggacggctcatatcgttcgtacctgggtgaagtcaccgtgccggccagtattgcaccgatgatcgaagcggttctgggcctggatacgcgtccggtcgcccgcccgcattttcgtatgcagcgtcgcgcagaaggcggtttcgaagctcgttcccaagcggcggcaccgaccgcatatacgccgctggatgttgcgcaggcctaccaatttccggaaggtctggacggccagggtcaatgcattgccattatcgaactgggcggtggctatgatgaagcttcactggcgcagtacttcgcgtcgctgggcgtgccggcaccgcaagtcgtgagtgtttccgtcgatggtgcgagcaaccagccgaccggtgatccgtctggtccggacggtgaagtggaactggatatcgaagttgcaggcgctctggcgccgggtgccaaatttgcagtgtatttcgcgccggataccgatgccggttttctggacgcgattaccacggccatccacgatccgacgctgaaaccgagcgttgtctcaatttcgtggagcggcccggaagacagctggacctctgctgcgatcgccgcaatgaaccgtgcgtttctggatgctgcggccctgggtgtgaccgttctggcagctgcgggcgacagcggttctacgggtggcgaacaggacggtctgtatcatgtgcatttcccggccgcatcaccgtacgttctggcgtgcggtggcacgcgcctggtcgcatcgggtggccgtattgcgcaggaaaccgtctggaacgatggtccggacggtggtgcaacgggtggcggtgtgagccgcatcttcccgctgccggcatggcaggaacacgctaacgttccgccgtctgcaaatccgggcgcgagcagcggccgtggtgtcccggatctggctggtaatgcggacccggcaaccggttatgaagtggttattgatggcgaagcaaccgtcatcggcggtacgagcgccgtggcaccgctgtttgctgcgctggttgcgcgtattaaccagaaactgggcaaagcagttggttatctgaatccgaccctgtaccaactgccggcagatgttttccatgacatcacggagggtaacaatgatattgcaaaccgtgcgcagatttatcaagcaggtccgggctgggacccgtgtaccggtctgggttcaccgattggtgtgcgtctgctgcaagcactgctgccgagtgcctcccagccgcaaccgggtagtacggaaaatctgtactttcagtccggcgcgctcgag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z