LOCUS       BBa_K1606007            2788 bp    DNA     linear       06-May-2024
DEFINITION  Kumamax enzyme that gluten updated m-cherry reporter device
ACCESSION   BBa_K1606007
VERSION     BBa_K1606007.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..944
                     /label=BBa_K1606029
     CDS             951..2651
                     /label=BBa_K590087
     stem_loop       2671..2714
                     /label=stem_loop
     misc_feature    2660..2739
                     /label=BBa_B0010
     terminator      2660..2739
                     /label=BBa_B0010
     stem_loop       2755..2774
                     /label=T7 TE
     misc_feature    2781..2781
                     /label=stop
     polyA_site      2775..2788
                     /label=polya
     misc_feature    2748..2788
                     /label=BBa_B0012
     terminator      2748..2788
                     /label=BBa_B0012
     terminator      2660..2788
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa ccacaacttc cagacggtgg tcactacgac gctgaagtta aaaccaccta
      781 catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat
      841 cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca
      901 ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgctactag atgagtgata
      961 tggaaaaacc gtggaaagaa ggtgaagaag cccgcgcagt gctgcaaggt catgctcgtg
     1021 cgcaggcacc gcaagcagtc gataaaggcc cggtggcagg tgacgaacgc atggctgtta
     1081 ccgtggttct gcgtcgccag cgtgcaggtg aactggcggc ccacgtggaa cgtcaagcag
     1141 ctattgctcc gcatgcgcgc gaacacctga aacgtgaagc gtttgcggcc agtcatggtg
     1201 cgtccctgga tgactttgcc gaactgcgtc gcttcgcaga tgctcacggc ctggcgctgg
     1261 accgtgcaaa cgttgcagct ggcaccgccg ttctgtctgg tccggtcgat gcaatcaatc
     1321 gcgcttttgg tgtggaactg cgtcatttcg atcacccgga cggctcatat cgttcgtacc
     1381 tgggtgaagt caccgtgccg gccagtattg caccgatgat cgaagcggtt ctgggcctgg
     1441 atacgcgtcc ggtcgcccgc ccgcattttc gtatgcagcg tcgcgcagaa ggcggtttcg
     1501 aagctcgttc ccaagcggcg gcaccgaccg catatacgcc gctggatgtt gcgcaggcct
     1561 accaatttcc ggaaggtctg gacggccagg gtcaatgcat tgccattatc gaactgggcg
     1621 gtggctatga tgaagcttca ctggcgcagt acttcgcgtc gctgggcgtg ccggcaccgc
     1681 aagtcgtgag tgtttccgtc gatggtgcga gcaaccagcc gaccggtgat ccgtctggtc
     1741 cggacggtga agtggaactg gatatcgaag ttgcaggcgc tctggcgccg ggtgccaaat
     1801 ttgcagtgta tttcgcgccg gataccgatg ccggttttct ggacgcgatt accacggcca
     1861 tccacgatcc gacgctgaaa ccgagcgttg tctcaatttc gtggagcggc ccggaagaca
     1921 gctggacctc tgctgcgatc gccgcaatga accgtgcgtt tctggatgct gcggccctgg
     1981 gtgtgaccgt tctggcagct gcgggcgaca gcggttctac gggtggcgaa caggacggtc
     2041 tgtatcatgt gcatttcccg gccgcatcac cgtacgttct ggcgtgcggt ggcacgcgcc
     2101 tggtcgcatc gggtggccgt attgcgcagg aaaccgtctg gaacgatggt ccggacggtg
     2161 gtgcaacggg tggcggtgtg agccgcatct tcccgctgcc ggcatggcag gaacacgcta
     2221 acgttccgcc gtctgcaaat ccgggcgcga gcagcggccg tggtgtcccg gatctggctg
     2281 gtaatgcgga cccggcaacc ggttatgaag tggttattga tggcgaagca accgtcatcg
     2341 gcggtacgag cgccgtggca ccgctgtttg ctgcgctggt tgcgcgtatt aaccagaaac
     2401 tgggcaaagc agttggttat ctgaatccga ccctgtacca actgccggca gatgttttcc
     2461 atgacatcac ggagggtaac aatgatattg caaaccgtgc gcagatttat caagcaggtc
     2521 cgggctggga cccgtgtacc ggtctgggtt caccgattgg tgtgcgtctg ctgcaagcac
     2581 tgctgccgag tgcctcccag ccgcaaccgg gtagtacgga aaatctgtac tttcagtccg
     2641 gcgcgctcga gtactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     2701 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     2761 ccttcgggtg ggcctttctg cgtttata
//