BBa_K1606029 1 BBa_K1606029 Gluten Updated m-cherry reporter gene 2015-08-26T11:00:00Z 2015-08-28T04:23:36Z It comes from genomic sequence and we add a sequence that square with the amino acid sequence of the gluten protein of PQLP amino acid sequence that is digested by Kumamax enzyme. we modernized m-cherry (BBa_J04450) part to report the absence of the gluten protein in a solution or in a dough that our updated Kumamax enzyme is inactive in the environment. When the absence of the protein of the gluten our repoter gene is expressed and give red light to environment. false false _2023_ 21244 21129 9 false atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacga gttcgaaatcgaaggtgaaggtg aaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggac atcctgtccccgcagttccagta cggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaat gggaacgtgttatgaacttcgaa gacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtgg taccaacttcccgtccgacggtc cggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggt gaaatcaaaatgcgtctgaaact gaaacca caa ctt ccagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaa aaccgacatcaaactggac atcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctta ataacgctgatagtgctagtgta gatcgc false Dorukcan ??zk??se BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K1606007 1 BBa_K1606007 Kumamax enzyme that gluten updated m-cherry reporter device 2015-08-26T11:00:00Z 2015-08-27T04:15:20Z It comes from the new part of BBa_K1606029 and the combinations of R0010,K590087,B0034,B0015 We modernized m-cherry (BBa_J06504) part to report the absence of the gluten protein in a solution or in a dough that our updated Kumamax enzyme is inactive in the environment. When the absence of the protein of the gluten our repoter gene is expressed and give red light to environment. Besides LacI promoter works under acidic environment in the dough formation. false false _2023_ 21129 21129 9 false It comes from the new part of BBa_K1606029 and the combinations of R0010,K590087,B0034,B0015 false Dorukcan ??zk??se component2438810 1 BBa_B0015 component2438801 1 BBa_B0034 component2438803 1 BBa_K590087 component2438793 1 BBa_R0010 component2438802 1 BBa_K1606029 annotation2438793 1 BBa_R0010 range2438793 1 1 200 annotation2438802 1 BBa_K1606029 range2438802 1 227 944 annotation2438801 1 BBa_B0034 range2438801 1 209 220 annotation2438810 1 BBa_B0015 range2438810 1 2660 2788 annotation2438803 1 BBa_K590087 range2438803 1 951 2651 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961227 1 start range1961227 1 173 173 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961225 1 -10 range1961225 1 161 166 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961224 1 -35 range1961224 1 137 142 BBa_K590087 1 KumaMax KumaMax: Kumamolisin-As_N291D, G319S, D358G, D368H 2011-09-21T11:00:00Z 2015-05-08T01:12:48Z Engineered but the original protein is form Alicyclobacillus sendaiensis A mutated Kumamolisin-As enzyme aimed to combat gluten intolerance by increased activity with the PQLP peptide, an antigenic epitope in gliadin. This mutant is the best combinatorial mutant found and has point mutations at residues 291, 319, 358, and 368 from Asparagine to Aspartate, Glycine to Serine, Aspartate to Glycine, and Aspartate to Histidine, respectively. false false _761_ 0 5005 9 It's complicated true Aimed to increase binding affinity to the immunogenic PQLP peptide found in gluten false Sydney Gordon, Daniel Hadidi, Elizabeth Stanley, Sarah Wolf, Angus Toland, Sean Wu BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1606007_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaaccacaacttccagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagatgagtgatatggaaaaaccgtggaaagaaggtgaagaagcccgcgcagtgctgcaaggtcatgctcgtgcgcaggcaccgcaagcagtcgataaaggcccggtggcaggtgacgaacgcatggctgttaccgtggttctgcgtcgccagcgtgcaggtgaactggcggcccacgtggaacgtcaagcagctattgctccgcatgcgcgcgaacacctgaaacgtgaagcgtttgcggccagtcatggtgcgtccctggatgactttgccgaactgcgtcgcttcgcagatgctcacggcctggcgctggaccgtgcaaacgttgcagctggcaccgccgttctgtctggtccggtcgatgcaatcaatcgcgcttttggtgtggaactgcgtcatttcgatcacccggacggctcatatcgttcgtacctgggtgaagtcaccgtgccggccagtattgcaccgatgatcgaagcggttctgggcctggatacgcgtccggtcgcccgcccgcattttcgtatgcagcgtcgcgcagaaggcggtttcgaagctcgttcccaagcggcggcaccgaccgcatatacgccgctggatgttgcgcaggcctaccaatttccggaaggtctggacggccagggtcaatgcattgccattatcgaactgggcggtggctatgatgaagcttcactggcgcagtacttcgcgtcgctgggcgtgccggcaccgcaagtcgtgagtgtttccgtcgatggtgcgagcaaccagccgaccggtgatccgtctggtccggacggtgaagtggaactggatatcgaagttgcaggcgctctggcgccgggtgccaaatttgcagtgtatttcgcgccggataccgatgccggttttctggacgcgattaccacggccatccacgatccgacgctgaaaccgagcgttgtctcaatttcgtggagcggcccggaagacagctggacctctgctgcgatcgccgcaatgaaccgtgcgtttctggatgctgcggccctgggtgtgaccgttctggcagctgcgggcgacagcggttctacgggtggcgaacaggacggtctgtatcatgtgcatttcccggccgcatcaccgtacgttctggcgtgcggtggcacgcgcctggtcgcatcgggtggccgtattgcgcaggaaaccgtctggaacgatggtccggacggtggtgcaacgggtggcggtgtgagccgcatcttcccgctgccggcatggcaggaacacgctaacgttccgccgtctgcaaatccgggcgcgagcagcggccgtggtgtcccggatctggctggtaatgcggacccggcaaccggttatgaagtggttattgatggcgaagcaaccgtcatcggcggtacgagcgccgtggcaccgctgtttgctgcgctggttgcgcgtattaaccagaaactgggcaaagcagttggttatctgaatccgaccctgtaccaactgccggcagatgttttccatgacatcacggagggtaacaatgatattgcaaaccgtgcgcagatttatcaagcaggtccgggctgggacccgtgtaccggtctgggttcaccgattggtgtgcgtctgctgcaagcactgctgccgagtgcctcccagccgcaaccgggtagtacggaaaatctgtactttcagtccggcgcgctcgagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_B0034_sequence 1 aaagaggagaaa BBa_K590087_sequence 1 atgagtgatatggaaaaaccgtggaaagaaggtgaagaagcccgcgcagtgctgcaaggtcatgctcgtgcgcaggcaccgcaagcagtcgataaaggcccggtggcaggtgacgaacgcatggctgttaccgtggttctgcgtcgccagcgtgcaggtgaactggcggcccacgtggaacgtcaagcagctattgctccgcatgcgcgcgaacacctgaaacgtgaagcgtttgcggccagtcatggtgcgtccctggatgactttgccgaactgcgtcgcttcgcagatgctcacggcctggcgctggaccgtgcaaacgttgcagctggcaccgccgttctgtctggtccggtcgatgcaatcaatcgcgcttttggtgtggaactgcgtcatttcgatcacccggacggctcatatcgttcgtacctgggtgaagtcaccgtgccggccagtattgcaccgatgatcgaagcggttctgggcctggatacgcgtccggtcgcccgcccgcattttcgtatgcagcgtcgcgcagaaggcggtttcgaagctcgttcccaagcggcggcaccgaccgcatatacgccgctggatgttgcgcaggcctaccaatttccggaaggtctggacggccagggtcaatgcattgccattatcgaactgggcggtggctatgatgaagcttcactggcgcagtacttcgcgtcgctgggcgtgccggcaccgcaagtcgtgagtgtttccgtcgatggtgcgagcaaccagccgaccggtgatccgtctggtccggacggtgaagtggaactggatatcgaagttgcaggcgctctggcgccgggtgccaaatttgcagtgtatttcgcgccggataccgatgccggttttctggacgcgattaccacggccatccacgatccgacgctgaaaccgagcgttgtctcaatttcgtggagcggcccggaagacagctggacctctgctgcgatcgccgcaatgaaccgtgcgtttctggatgctgcggccctgggtgtgaccgttctggcagctgcgggcgacagcggttctacgggtggcgaacaggacggtctgtatcatgtgcatttcccggccgcatcaccgtacgttctggcgtgcggtggcacgcgcctggtcgcatcgggtggccgtattgcgcaggaaaccgtctggaacgatggtccggacggtggtgcaacgggtggcggtgtgagccgcatcttcccgctgccggcatggcaggaacacgctaacgttccgccgtctgcaaatccgggcgcgagcagcggccgtggtgtcccggatctggctggtaatgcggacccggcaaccggttatgaagtggttattgatggcgaagcaaccgtcatcggcggtacgagcgccgtggcaccgctgtttgctgcgctggttgcgcgtattaaccagaaactgggcaaagcagttggttatctgaatccgaccctgtaccaactgccggcagatgttttccatgacatcacggagggtaacaatgatattgcaaaccgtgcgcagatttatcaagcaggtccgggctgggacccgtgtaccggtctgggttcaccgattggtgtgcgtctgctgcaagcactgctgccgagtgcctcccagccgcaaccgggtagtacggaaaatctgtactttcagtccggcgcgctcgag BBa_K1606029_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaaccacaacttccagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z