LOCUS       BBa_K1606010            1081 bp    DNA     linear       22-Nov-2024
DEFINITION  Kumamax enzyme that gluten updated m-cherry reporter device
ACCESSION   BBa_K1606010
VERSION     BBa_K1606010.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..944
                     /label=BBa_K1606029
     stem_loop       964..1007
                     /label=stem_loop
     misc_feature    953..1032
                     /label=BBa_B0010
     terminator      953..1032
                     /label=BBa_B0010
     stem_loop       1048..1067
                     /label=T7 TE
     misc_feature    1074..1074
                     /label=stop
     polyA_site      1068..1081
                     /label=polya
     misc_feature    1041..1081
                     /label=BBa_B0012
     terminator      1041..1081
                     /label=BBa_B0012
     terminator      953..1081
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa ccacaacttc cagacggtgg tcactacgac gctgaagtta aaaccaccta
      781 catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat
      841 cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca
      901 ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgctactag agccaggcat
      961 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1021 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1081 a
//