LOCUS       BBa_K1606014            1248 bp    DNA     linear       22-Dec-2024
DEFINITION  Strong promoter without thickener of LuxR and antiholin sequence
ACCESSION   BBa_K1606014
VERSION     BBa_K1606014.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     CDS             65..358
                     /label=BBa_K112006
     misc_feature    365..366
                     /label=prefix
     misc_feature    538..538
                     /label=T
     misc_feature    856..856
                     /label=A
     CDS             365..1114
                     /label=luxR
     misc_feature    365..1120
                     /label=BBa_C0062
     misc_feature    1121..1145
                     /label=Help:Barcodes
     CDS             365..1120
                     /label=BBa_C0062
     stem_loop       1161..1180
                     /label=T7 TE
     misc_feature    1187..1187
                     /label=stop
     polyA_site      1181..1194
                     /label=polya
     misc_feature    1154..1194
                     /label=BBa_B0012
     terminator      1154..1194
                     /label=BBa_B0012
     stem_loop       1215..1237
                     /label=stem_loop
     misc_feature    1203..1248
                     /label=BBa_B0011
     terminator      1203..1248
                     /label=BBa_B0011
     terminator      1154..1248
                     /label=BBa_B0014
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagattaaag aggagaaata
       61 ctagatggcc ttaaaagcaa cagcactttt tgccatgcta ggattgtcat ttgttttatc
      121 tccatcgatt gaagcgaatg tcgatcctca ttttgataaa tttatggaat ctggtattag
      181 gcacgtttat atgctttttg aaaataaaag cgtagaatcg tctgaacaat tctatagttt
      241 tatgagaacg acctataaaa atgacccgtg ctcttctgat tttgaatgta tagagcgagg
      301 cgcggagatg gcacaatcat acgctagaat tatgaacatt aaattggaga ctgaatgata
      361 ctagatgaaa aacataaatg ccgacgacac atacagaata attaataaaa ttaaagcttg
      421 tagaagcaat aatgatatta atcaatgctt atctgatatg actaaaatgg tacattgtga
      481 atattattta ctcgcgatca tttatcctca ttctatggtt aaatctgata tttcaatcct
      541 agataattac cctaaaaaat ggaggcaata ttatgatgac gctaatttaa taaaatatga
      601 tcctatagta gattattcta actccaatca ttcaccaatt aattggaata tatttgaaaa
      661 caatgctgta aataaaaaat ctccaaatgt aattaaagaa gcgaaaacat caggtcttat
      721 cactgggttt agtttcccta ttcatacggc taacaatggc ttcggaatgc ttagttttgc
      781 acattcagaa aaagacaact atatagatag tttattttta catgcgtgta tgaacatacc
      841 attaattgtt ccttctctag ttgataatta tcgaaaaata aatatagcaa ataataaatc
      901 aaacaacgat ttaaccaaaa gagaaaaaga atgtttagcg tgggcatgcg aaggaaaaag
      961 ctcttgggat atttcaaaaa tattaggttg cagtgagcgt actgtcactt tccatttaac
     1021 caatgcgcaa atgaaactca atacaacaaa ccgctgccaa agtatttcta aagcaatttt
     1081 aacaggagca attgattgcc catactttaa aaattaataa cactgatagt gctagtgtag
     1141 atcactacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
     1201 agagagaata taaaaagcca gattattaat ccggcttttt tattattt
//