LOCUS       BBa_K1606020            1416 bp    DNA     linear       22-May-2024
DEFINITION  Lux promoter with holin and endolysin kill switch codin sequence
ACCESSION   BBa_K1606020
VERSION     BBa_K1606020.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    52..68
                     /label=cI (OR1)
     misc_feature    53..53
                     /label=start
     misc_feature    1..68
                     /label=BBa_I1051
     promoter        1..68
                     /label=BBa_I1051
     misc_feature    89..91
                     /label=start codon
     RBS             77..94
                     /label=BBa_J63003
     CDS             101..757
                     /label=BBa_K112000
     CDS             782..1276
                     /label=T4 Endolysin
     CDS             766..1279
                     /label=BBa_K112806
     stem_loop       1299..1342
                     /label=stem_loop
     misc_feature    1288..1367
                     /label=BBa_B0010
     terminator      1288..1367
                     /label=BBa_B0010
     stem_loop       1383..1402
                     /label=T7 TE
     misc_feature    1409..1409
                     /label=stop
     polyA_site      1403..1416
                     /label=polya
     misc_feature    1376..1416
                     /label=BBa_B0012
     terminator      1376..1416
                     /label=BBa_B0012
     terminator      1288..1416
                     /label=BBa_B0015
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga atacctctgg
       61 cggtgatata ctagagcccg ccgccaccat ggagtactag atggcagcac ctagaatatc
      121 attttcgccc tctgatattc tatttggtgt tctcgatcgc ttgttcaaag ataacgctac
      181 cgggaaggtt cttgcttccc gggtagctgt cgtaattctt ttgtttataa tggcgattgt
      241 ttggtatagg ggagatagtt tctttgagta ctataagcaa tcaaagtatg aaacatacag
      301 tgaaattatt gaaaaggaaa gaactgcacg ctttgaatct gtcgccctgg aacaactcca
      361 gatagttcat atatcatctg aggcagactt tagtgcggtg tattctttcc gccctaaaaa
      421 cttaaactat tttgttgata ttatagcata cgaaggaaaa ttaccttcaa caataagtga
      481 aaaatcactt ggaggatatc ctgttgataa aactatggat gaatatacag ttcatttaaa
      541 tggacgtcat tattattcca actcaaaatt tgctttttta ccaactaaaa agcctactcc
      601 cgaaataaac tacatgtaca gttgtccata ttttaatttg gataatatct atgctggaac
      661 gataaccatg tactggtata gaaatgatca tataagtaat gaccgccttg aatcaatatg
      721 tgctcaggcg gccagaatat taggaagggc taaataatac tagagatact taggaggtat
      781 tatgaatata tttgaaatgt tacgtataga tgaaggtctt agacttaaaa tctataaaga
      841 cacagaaggc tattacacta ttggcatcgg tcatttgctt acaaaaagtc catcacttaa
      901 tgctgctaaa tctgaattag ataaagctat tgggcgtaat tgcaatggtg taattacaaa
      961 agatgaggct gaaaaactct ttaatcagga tgttgatgct gctgttcgcg gaatcctgag
     1021 aaatgctaaa ttaaaaccgg tttatgattc tcttgatgcg gttcgtcgct gtgcattgat
     1081 taatatggtt ttccaaatgg gagaaaccgg tgtggcagga tttactaact ctttacgtat
     1141 gcttcaacaa aaacgctggg atgaagcagc agttaactta gctaaaagta gatggtataa
     1201 tcaaacacct aatcgcgcaa aacgagtcat tacaacgttt agaactggca cttgggacgc
     1261 gtataaaaat ctataaagct actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1321 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1381 ctggctcacc ttcgggtggg cctttctgcg tttata
//