BBa_K1607011
1
BBa_K1607011
The SCFV of anti-p185her2/neu antibody chA21 linked with EGFP by a (Gly4Ser)3 linker
2015-08-25T11:00:00Z
2015-08-26T08:57:56Z
LCR assembly and PCR.(detailed description will be provided soon)
The anti-p185her2/neu antibody chA21 mediates specific inhibitory effects on p185her2/neu-overexpressed cancer cells, as well as human breast and ovarian cancer xenograft [1]
This part is the coding sequence of chA21 SCFV, fused with EGFP.A (Gly4Ser)3 linker was inserted. The SCFV should be able to specifically bind to tumor antigen p185her2/neu-ECD (???ECD??? stands for ???extracellular domain???).
false
false
_2024_
25365
25365
9
false
design information will be provided soon
false
Jiazheng Xing
annotation2438124
1
chA21SCFV
range2438124
1
1
585
annotation2438126
1
EGFP
range2438126
1
631
1347
annotation2438125
1
(Gly4Ser)3 linker
range2438125
1
586
630
BBa_K1607011_sequence
1
atgggttctactcaagtttgtactggtactgatatgaagttgagattgccagcttctccagaaactcatttggatatgttgagacatttgtaccaaggttgtcaagttgttcaaggtaacttggaattgacttacttgccaactaacgcttctttgtcttttttgcaagatattcaagaagttcaaggttacgttttgattgctcataaccaagttagacaagttccattgcaaagattgagaattgttagaggtactcaattgtttgaagataactacgctttggctgttttggataacggtgatccattgaacaacactactccagttactggtgcttctccaggtggtttgagagaattgcaattgagatctttgactgaaattttgaagggtggtgttttgattcaaagaaacccacaattgtgttaccaagatactattttgtggaaggatatttttcataagaacaaccaattggctttgactttgattgatactaacagatccagagcttgtcatccatgttctccaatgtgtaagggttccagatgttggggtgaatcttctgaagattgtcaatctggcggtggcggttctggaggcggaggcagcggtggcggtggaagcatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z