LOCUS       BBa_K1608003            2256 bp    DNA     linear       07-May-2025
DEFINITION  SRPK-His/pSB1C3
ACCESSION   BBa_K1608003
VERSION     BBa_K1608003.1  
FEATURES             Location/Qualifiers
     promoter        51..67
                     /label=T7 promoter
     misc_feature    72..92
                     /label=lac operator
     misc_feature    142..186
                     /label=S-Tag coding sequence
     CDS             231..2196
                     /label=SRPK1
     misc_feature    2236..2253
                     /label=His-Tag coding sequence
ORIGIN
        1 cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact
       61 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt
      121 taagaaggag atatacatat gaaagaaacc gctgctgcta aattcgaacg ccagcacatg
      181 gacagcccag atctgggtac cctggtgcca cgcggttcca tggcgatatc ggatcgatcc
      241 aaagtgcttg cgctccaggc ccgaaagaaa aggaccaagg ccaagaagga caaagcccaa
      301 aggaaatctg aaactcagca ccgaggctct gctccccact ctgagagtga tctaccagag
      361 caggaagagg agattctggg atctgatgat gatgagcaag aagatcctaa tgattattgt
      421 aaaggaggtt atcatcttgt gaaaattgga gatctattca atgggagata ccatgtgatc
      481 cgaaagttag gctggggaca cttttcaaca gtatggttat catgggatat tcaggggaag
      541 aaatttgtgg caatgaaagt agttaaaagt gctgaacatt acactgaaac agcactagat
      601 gaaatccggt tgctgaagtc agttcgcaat tcagacccta atgatccaaa tagagaaatg
      661 gttgttcaac tactagatga ctttaaaata tcaggagtta atggaacaca tatctgcatg
      721 gtatttgaag ttttggggca tcatctgctc aagtggatca tcaaatccaa ttatcagggg
      781 cttccactgc cttgtgtcaa aaaaattatt cagcaagtgt tacagggtct tgattattta
      841 cataccaagt gccgtatcat ccacactgac attaaaccag agaacatctt attgtcagtg
      901 aatgagcagt acattcggag gctggctgca gaagcaacag aatggcagcg atctggagct
      961 cctccgcctt ccggatctgc agtcagtact gctccccagc ctaaaccagc tgacaaaatg
     1021 tcaaagaata agaagaagaa attgaagaag aagcagaagc gccaggcaga attactagag
     1081 aagcgaatgc aggaaattga ggaaatggag aaagagtcgg gccctgggca aaaaagacca
     1141 aacaagcaag aagaatcaga gagtcctgtt gaaagaccct tgaaagagaa cccacctaat
     1201 aaaatgaccc aagaaaaact tgaagagtca agtaccattg gccaggatca aacgcttatg
     1261 gaacgtgata cagagggtgg tgcagcagaa attaattgca atggagtgat tgaagtcatt
     1321 aattatactc agaacagtaa taatgaaaca ttgagacata aagaggatct acataatgct
     1381 aatgactgtg atgtccaaaa tttgaatcag gaatctagtt tcctaagctc ccaaaatgga
     1441 gacagcagca catctcaaga aacagactct tgtacaccta taacatctga ggtgtcagac
     1501 accatggtgt gccagtcttc ctcaactgta ggtcagtcat tcagtgaaca acacattagc
     1561 caacttcaag aaagcattcg ggcagagata ccctgtgaag atgaacaaga gcaagaacat
     1621 aacggaccac tggacaacaa aggaaaatcc acggctggaa attttcttgt taatcccctt
     1681 gagccaaaaa atgcagaaaa gctcaaggtg aagattgctg accttggaaa tgcttgttgg
     1741 gtgcacaaac atttcactga agatattcaa acaaggcaat atcgttcctt ggaagttcta
     1801 atcggatctg gctataatac ccctgctgac atttggagca cggcatgcat ggcctttgaa
     1861 ctggccacag gtgactattt gtttgaacct cattcagggg aagagtacac tcgagatgaa
     1921 gatcacattg cattgatcat agaacttctg gggaaggtgc ctcgcaagct cattgtggca
     1981 ggaaaatatt ccaaggaatt tttcaccaaa aaaggtgacc tgaaacatat cacgaagctg
     2041 aaaccttggg gcctttttga ggttctagtg gagaagtatg agtggtctca ggaagaggca
     2101 gctggcttca cagatttctt actgcccatg ttggagctga tccctgagaa gagagccact
     2161 gccgccgagt gtctccggca cccttggctt aactccaatt cgagctccgt cgacaagctt
     2221 gcggccgcac tcgagcacca ccaccaccac cactga
//