BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1610101
1
BBa_K1610101
pTS + RBS
2015-09-05T11:00:00Z
2015-09-06T12:07:42Z
This part utilizes the already existing temperature sensitive promoter (Bba_K608351).
This party consists of a temperature sensitive promoter (Bba_K608351) and a RBS (Bba_B0034). The temperature sensitive promoter activates at and above 37 degrees Celsius. The addition of the RBS behind the temperature sensitive promoter means an ORF can be easily inserted downstream of this part and activated in a high temperature environment at and above 37C.
false
false
_2027_
22834
22834
9
false
None.
false
Leon Yim
component2444436
1
BBa_B0034
component2444418
1
BBa_B0034
component2444434
1
BBa_K608351
annotation2444436
1
BBa_B0034
range2444436
1
957
968
annotation2444434
1
BBa_K608351
range2444434
1
1
948
annotation2444418
1
BBa_B0034
range2444418
1
44
55
BBa_K608351
1
BBa_K608351
(correct) temperature sensitive promoter
2011-09-13T11:00:00Z
2015-05-08T01:12:52Z
asd
Released HQ 2013
correct temperatursesitive promotor
false
false
_780_
0
9113
9
In stock
false
asd
false
Theodhor Tiko
annotation2131305
1
BBa_B0012
range2131305
1
797
837
annotation2131303
1
cIts
range2131303
1
62
775
annotation2131314
1
OR1
range2131314
1
924
940
annotation2131312
1
BBa_R0051
range2131312
1
900
948
annotation2131304
1
BBa_K098997
range2131304
1
62
775
annotation2131302
1
BBa_B0034
range2131302
1
44
55
annotation2131310
1
stem_loop
range2131310
1
858
880
annotation2131308
1
stop
range2131308
1
830
830
annotation2131309
1
BBa_B0011
range2131309
1
846
891
annotation2131307
1
polya
range2131307
1
824
837
annotation2131425
1
conserved
range2131425
1
776
788
annotation2131301
1
conserved
range2131301
1
48
51
annotation2131311
1
OR2
range2131311
1
900
916
annotation2131300
1
BBa_J23114
range2131300
1
1
35
annotation2131315
1
-10
range2131315
1
937
942
annotation2131306
1
T7 TE
range2131306
1
804
823
annotation2131313
1
-35
range2131313
1
914
919
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1610101_sequence
1
tttatggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctgactccagcggccgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaa
BBa_K608351_sequence
1
tttatggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctgactccagcggccgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z