This part consists of parts ordered from iGEM parts registry (Bba_K608351, Bba_B0034, Bba_B0015) and a human cDNA clone obtained from Sino Biological (Bba_K1610200).
This part consists of temperature sensitive promoter (Bba_K608351), a RBS (Bba_B0034), a coding sequence (Bba_K1610200), and a terminator (Bba_B0015). This part is used to produce human antichymotrypsin, a human extracellular protein. This circuit is temperature regulated, where environments at or above 37C will activate the promoter.
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Double terminator consisting of BBa_B0010 and BBa_B0012
This part was obtained as a cDNA from Sino Biological. It is naturally found in humans.
Human Antichymotrypsin is a naturally occurring human extracellular protein.
(correct) temperature sensitive promoter
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1