LOCUS       BBa_K1610104            2383 bp    DNA     linear       22-Nov-2024
DEFINITION  pTS + RBS + ACT3m + Term
ACCESSION   BBa_K1610104
VERSION     BBa_K1610104.1  
FEATURES             Location/Qualifiers
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     stem_loop       804..823
                     /label=T7 TE
     misc_feature    830..830
                     /label=stop
     polyA_site      824..837
                     /label=polya
     misc_feature    797..837
                     /label=BBa_B0012
     terminator      797..837
                     /label=BBa_B0012
     misc_feature    1..35
                     /label=BBa_J23114
     misc_feature    48..51
                     /label=conserved
     misc_feature    44..55
                     /label=BBa_B0034
     CDS             62..775
                     /label=cIts
     misc_feature    62..775
                     /label=BBa_K098997
     misc_feature    797..837
                     /label=BBa_B0012
     stem_loop       804..823
                     /label=T7 TE
     polyA_site      824..837
                     /label=polya
     misc_feature    830..830
                     /label=stop
     misc_feature    846..891
                     /label=BBa_B0011
     stem_loop       858..880
                     /label=stem_loop
     misc_feature    900..916
                     /label=OR2
     misc_feature    900..948
                     /label=BBa_R0051
     promoter        914..919
                     /label=-35
     misc_feature    924..940
                     /label=OR1
     promoter        937..942
                     /label=-10
     misc_feature    776..788
                     /label=conserved
     misc_feature    1..948
                     /label=BBa_K608351
     misc_feature    961..964
                     /label=conserved
     RBS             957..968
                     /label=BBa_B0034
     CDS             975..2246
                     /label=BBa_K1610201
     stem_loop       2266..2309
                     /label=stem_loop
     misc_feature    2255..2334
                     /label=BBa_B0010
     terminator      2255..2334
                     /label=BBa_B0010
     stem_loop       2350..2369
                     /label=T7 TE
     misc_feature    2376..2376
                     /label=stop
     polyA_site      2370..2383
                     /label=polya
     misc_feature    2343..2383
                     /label=BBa_B0012
     terminator      2343..2383
                     /label=BBa_B0012
     terminator      2255..2383
                     /label=BBa_B0015
ORIGIN
        1 tttatggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaatacta
       61 gatgagcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac gtcgccttaa
      121 agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg tcgcagacaa
      181 gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg cattaaatgc
      241 ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat ttagcccttc
      301 aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt cacttagaag
      361 tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac ctgagcttag
      421 aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag ccagtgattc
      481 tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct ccaagccaag
      541 ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc caggtgattt
      601 ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca gggatagcgg
      661 tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca atgagagttg
      721 ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg gctgactcca
      781 gcggccgcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac
      841 tagagagaga atataaaaag ccagattatt aatccggctt ttttattatt ttactagagt
      901 aacaccgtgc gtgttgacta ttttacctct ggcggtgata atggttgcta ctagagaaag
      961 aggagaaata ctagatggag agaatgttac ctctcctggc tctggggctc ttggcggctg
     1021 ggttctgccc tgctgtcctc tgccacccta acagcccact tgacgaggag aatctgaccc
     1081 aggagaacca agaccgaggg acacacgtgg acctcggatt agcctccgcc aacgtggact
     1141 tcgctttcag cctgtacaag cagttagtcc tgaaggcccc tgataagaat gtcatcttct
     1201 ccccactgag catctccacc gccttggcct tcctgtctct gggggcccat aataccaccc
     1261 tgacagagat tctcaaaggc ctcaagttca acctcacgga gacttctgag gcagaaattc
     1321 accagagctt ccagcacctc ctgcgcaccc tcaatcagtc cagcgatgag ctacagctga
     1381 gtatgggaaa tgccatgttt gtcaaagagc aactcagtct gctggacagg ttcacggagg
     1441 atgccaagag gctgtatggc tccgaggcct ttgccactga ctttcaggac tcagctgtag
     1501 ctaagaagct catcaacgac tacgtgaaga atggaactag ggggaaaatc acagatctga
     1561 tcaaggacct tgactcgcag acaatgatgg tcctggtgaa ttacatcttc tttaaagcca
     1621 aatgggagat gccctttgac ccccaagata ctcatcagtc aaggttctac ttgagcaaga
     1681 aaaagtgggt aatggtgccc atgatgagtt tgcatcacct gactatacct tacttccggg
     1741 acgaggagct gtcctgcacc gtggtggagc tgaagtacac aggcaatgcc agcgcactct
     1801 tcatcctccc tgatcaagac aagatggagg aagtggaagc catgctgctc ccagagaccc
     1861 tgaagcggtg gagagactct ctggagttca gagagatagg tgagctctac ctgccaaagt
     1921 tttccatctc gagggactat aacctgaacg acatacttct ccagctgggc attgaggaag
     1981 ccttcaccag caaggctgac ctgtcaggga tcacaggggc caggaaccta gcagtctccc
     2041 aggtggtcca taaggctgtg cttgatgtat ttgaggaggg cacagaagcg tctgctgcca
     2101 cagcagtcaa aatcgtcctc atgtctgcat tactggagac aaggaccatt gtgcgtttca
     2161 acaggccctt cctgatgatc attgtcccta cagacaccca gaacatcttc ttcatgagca
     2221 aagtcaccaa tcccaagcaa gcctagtact agagccaggc atcaaataaa acgaaaggct
     2281 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     2341 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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