This part consists of parts ordered from iGEM parts registry (Bba_K608351, Bba_B0034, Bba_B0015) and a human cDNA clone obtained from Sino Biological then modified by Mission Biotech (Bba_K1610201).
This part consists of temperature sensitive promoter (Bba_K608351), a RBS (Bba_B0034), a coding sequence (Bba_K1610201), and a terminator (Bba_B0015). This part is used to produce a modified human antichymotrypsin, a human extracellular protein that has been given the ability to bind and inhibit Granzyme B. This circuit is temperature regulated, where environments at or above 37C will activate the promoter.
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Double terminator consisting of BBa_B0010 and BBa_B0012
human Antichymotrypsin with mutations for 3 amino acid changes (ACT3m)
This part was derived off of Bba_K1610200, a cDNA clone provided by Sino Biological. The mutations on the sequence are later made by Mission Biotech.
This part is similar to human Antichymotrypsin (ACT) (Bba_K1610200) except it contains selected mutations. The mutations were made in order to allow ACT3m to bind and inhibit Granzyme B, a human enzyme that is found in the extracellular matrix, extracellularly.
There were originally two PstI cutting sites that have been mutated off (silent mutations).
(correct) temperature sensitive promoter
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1